ارزیابی تغییرات بیان ژن کاتاپسین L و فاکتورهای رشد در میگوی سفید غربی (Litopenaeus vannamei) تحت تاثیر بایوفلاک های متفاوت

نوع مقاله : ژنتیک

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر، ایران

2 گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه خلیج فارس، بوشهر، ایران

3 پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

کاتاپسین­ ها جزء اصلی سیستم پروتئولیتیک لیزوزومی و مسئول تجزیه پروتئین­ های داخل سلولی هستند. هدف این تحقیق ارزیابی تغییرات میزان بیان ژن کاتاپسین ال (CTSL) و فاکتورهای رشد در میگوی سفید غربی (Litopenaeus vannamei) تحت تاثیر بایوفلاک ­های متفاوت با استفاده از سطوح مختلف پروتئینی بود. چهار تیمار بایوفلاک مشتمل بر بایوفلاک خرما +جیره با پروتئین 25% (P25)، بایوفلاک ملاس+جیره با پروتئین 25% (M25)، بایوفلاک خرما+جیره با پروتئین 15% (P15) و بایوفلاک ملاس+جیره با پروتئین 15% (M15) و یک تیمار شاهد بدون بایوفلاک (38% پروتئین) با 3 تکرار بود. در یک دوره 35 روزه 35 قطعه میگوی جوان (0/33±5/37 گرم) به ­طور تصادفی در 15 مخزن 300 لیتری (تراکم 175 قطعه در متر مکعب) ذخیره ­سازی گردید. به ­منظور برآورد تاثیر بایوفلاک بر میزان بیان ژن کاتاپسین (L (CTSL  در هپاتوپانکراس میگو از تکنیک Real time PCR و از ژن Beta actin به ­عنوان ژن کنترل داخلی استفاده شد. در تمام تیمارهای بایوفلاک نسبت به شاهد افزایش معنی ­داری در میزان بیان ژن CTSL مشاهده گردید (0/05>P)، هرچندکه تیمار 25P بالاترین میزان بیان را نشان داد (0/05>P). هم­ چنین بالاترین میزان پارامترهای رشد (وزن به­ دست آمده، ضریب تبدیل غذایی مطلوب، نرخ رشد و بازماندگی) در میگوهای تیمار P25 و کم­ترین آن ها در تیمار شاهد دیده شد (0/05>P). نتایج نشان داد میزان بیان ژن CTSL با تغییر منابع کربنی یا میزان پروتئین جیره در بین تیمارهای بایوفلاک، اختلاف معنی ­داری با یکدیگر نداشتند (0/05<P). به ­نظر می ­رسد فناوری بایوفلاک با اثرات مثبت خود بر گوارش و ایمنی، سبب افزایش بیان ژن CTSL در این گونه شده است.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of the Cathepsin-L gene expression changes and growth factors in Litopenaeus vannamei under different biofloc systems

نویسندگان [English]

  • Akbar Abbaszadeh 1
  • Vahid Yavari 1
  • Seyed Javad hoseini 2
  • Mahmoud Nafisi bahabadi 3
1 Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2 Department of Fisheries, Faculty of Agriculture and Natural Resources, University of the Persian Gulf, Bushehr, Iran
3 Persian Gulf Research Institute, Persian Gulf University, Bushehr, Iran
چکیده [English]

Cathepsins represent a major component of the lysosomal proteolytic system and, as such, are responsible for intracellular protein degradation. The aim of this study was to evaluate the cathepsin-L (CTSL) gene expression changes and growth factors in Litopenaeus vannamei  under various biofloc systems (BFT) and different protein levels. Four bioflocs were used: BFT fed diets of 25% CP and spoilage palm date extract (P25), diets of 25% CP and molasses (M25), diets of 15% CP and spoilage palm date extract (P15), diets of 15% CP and molasses (M15) and clear water without biofloc fed with 38% CP (Control) that were performed in triplicate. A 35-day study was conducted with 35 juvenile (average 5.37±0.33 g) shrimp randomly stocked in fifteen 300 L tanks at a stocking density of 175 shrimp m−3.  Real time PCR technique was used to estimate the effect of biofloc on the CTSL gene expression in shrimp hepatopancreas and Beta actin gene was used as an internal control gene. In all the biofloc treatments, a significant increase in the expression of CTSL gene was observed (P <0.05), although P25 had the highest expression (P> 0.05). Moreover the highest growth parameters (weight gain, better feed conversion ratio (FCR), growth rate and survival) was showed in P25 and the lowest in the control (P <0.05). The results showed that there were no significant differences in the expression of CTSL gene in change of carbon sources or dietary protein in the biofloc treatments (P> 0.05). It seems the biofloc technology with its positive effects on digestion and immunity provides the basis of increasing the CTSL gene expression in this species.

کلیدواژه‌ها [English]

  • Litopenaeus vannamei
  • Genetic
  • Biofloc
  • Date palm extract
  • Molasses
  • Growth

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فصلنامه محیط زیست جانوری
دوره 10، شماره 4
دی 1397
صفحه 467-476

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