مطالعه انگل های خونی در قورباغه مردابی Pelophylax ridibundus در شمال ایران

نوع مقاله : زیست شناسی (جانوری)

نویسندگان

گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

چکیده

 بی ­دمان گونه ­هایی از دوزیستان نیمه ­آبزی هستند که انگل­ های خونی مختلفی را متحمل می ­شوند. در این مطالعه در مجموع 66 قورباغه مردابی بالغ از شمال ایران بین اپریل و جولای سال 2017 به ­منظور بررسی فون انگل­ های خونی در آن­ ها گرفته شد. نمونه­ های خون با توجه به اندازه قورباغه از ورید صورت یا قلب گرفته شد. اسمیرهای خونی خشک شدند، با متانول فیکس شده و توسط گیمسا رنگ­ آمیزی شدند. نمونه ­ها توسط میکروسکوپ نوری با استفاده از بزرگ ­نمایی 100 و روغن ایمرسیون به­ منظور جستجوی انگل­ های خونی مورد آزمایش قرار گرفتند. فون انگل­ های خونی شامل ریکتزیاها و هموگری گارینی ­های داخل گلبول قرمز و میکروفیلاریا و اشکال مختلف تریپانوزوم در خارج گلبول قرمز خون مشاهده شد. این اولین مطالعه جامع مورفولوژیکی روی فون انگل­ های خونی قورباغه مردابی در ایران است، مطالعه­ ای که انتظار می ­رود استفاده از آنالیزهای مولکولی به ­منظور شناخت بهتر این انگل­ های خونی، ناقل­ های احتمالی و دینامیک سیکل زندگی آن­ ها را توسعه دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Study of blood parasites in marsh frog (Pelophylax ridibundus) in north of Iran

نویسندگان [English]

  • Fariba Rajabi
  • Hossein Javanbakht
Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran
چکیده [English]

Anuranare semi- aquatic species of amphibian, harbored different speciesof blood parasites. In this study a total of 66 adult marsh frogs were captured from north of Iran between April- July 2017 in order to investigate for blood parasites fauna. Samples of blood were collected from the facial vein or by cardiac puncture depending on the size of the frogs. The blood smears were dried, fixed with methanol and stained with Gimsa solution. Samples were examined by light microscopy using a 100 × magnification lens and immersion oil in search for hemoparasites. Their hematozoan fauna included intraerythrocytic Rickettsials and Haemogregarines as well as interearythrocytic microfilariae and different morphologically distincttypes of trypanosomes. This is the first comprehensive morphological study of blood parasites within the marsh frog from Iran, a study hoped to encourage the use of phylogenetic analysis that will give a better indication of these parasites possible vectors and life cycle dynamics.

کلیدواژه‌ها [English]

  • Marsh frog
  • blood parasite
  • Trypanosome
  • Haemogregarine
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