اثر غلظت های تحت کشنده نانوذرات نقره و نیترات نقره بر ماهی کپورمعمولی (Cyprinus carpio. L): تغییرات خون شناسی و آنتی اکسیدانی

نوع مقاله : فیزیولوژی (جانوری)

نویسندگان

گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

با توجه به کاربرد بالای نقره و هم­ چنین احتمال در معرض گرفتن آبزیان، امکان در معرض قرارگیری این موجودات با این فلز زیاد است. لذا این مطالعه با هدف بررسی و مقایسه اثرات تحت حاد نانوذرات نقره و نیترات نقره بر بقاء، شاخص ­های مختلف خونی و هم­ چنین آنزیم ­های شاخص آنتی­ اکسیدانی در ماهی کپورمعمولی انجام شد. ابتدا غلظت کشندگی 50% و 96 ساعته برای دو شکل نقره به ­دست آمد و سپس ماهیان به ­مدت 21 روز در معرض %15 و 30% از غلظت حاد محاسبه شده (0/4 و 0/8 میلی­ گرم در لیتر نانوذرات و هم­ چنین 0/025 و 0/045 میلی­ گرم در لیتر نیترات نقره) قرار گرفتند. متوسط غلظت حاد 96 ساعته برای نانوذرات نقره و نیترات نقره به ­ترتیب 0/02±0/29 و 0/05±0/15 میلی­ گرم در لیتر بود. نتایج نشان ­دهنده اختلالات مختلفی در شاخص ­های خون­ شناسی ازجمله کاهش تعداد گلبول­ های قرمز و افزایش تعداد گلبول­ های سفید بود. هم ­چنین آنزیم SOD در اکثر تیمارهای درمعرض قرار گرفته در مقایسه با گروه شاهد القاء شد. این مطالعه نشان داد نیترات نقره در غلظت های پایین ­تری اثرات کشندگی خود را اعمال می­ کند و هر دو شکل نقره تقریباً به شکل یکسان باعث تغیییرات در فاکتورهای ایمنی ذاتی و شاخص ­های استرس اکسیداتیو در ماهیان درمعرض قرار گرفته شدند اما در آنزیم SOD این تغییرات در گروه­ های مواجه شده با نیترات نقره کمی بیش ­تر بود. 

کلیدواژه‌ها

عنوان مقاله [English]

The effects of sub-lethal concentrations of silver nanoparticles and silver nitrate on common carp (Cyprinus carpio L.): changes in hematology and antioxidants

نویسندگان [English]

  • Kheyrollah Khosravi Katuli
  • Ali Shabani
  • Hamed Paknejad
  • Mohammad Reza Imanpour
Department of Fishery, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Considering the high application of silver as well as the risk of exposure of aquatic organisms, the possibility of exposing these animals to this metal is high. Therefore, the aim of this study was to investigate and compare the sub-acute effects of silver nanoparticle and silver nitrate on survival, various indices of blood and also antioxidant biomarker in common carp. For this purpose, at first, Lethal Concentration (LC50-96 h) for two silver forms was obtained and then fish were exposed to 15 and 30% of the calculated concentration (0.4 and 0.8 mg / L nanoparticles as well as 0.025 and 0.045 mg / L silver nitrate) for 21 days. LC50-96 h for silver nanoparticles and silver nitrate were 0.29 ± 0.02 and 0.15 ± 0.05 mg/L respectively. The results showed that after exposure to silver nanoparticles and silver nitrate, different disorders were observed in hematologic indices including reducing the RBC and WBC count. The results showed that the SOD enzyme activity in most exposed groups were induced in compare to the control group. This study showed that silver nitrate at lower concentrations show its destructive effects and both forms of silver almost identically changed immunity factors and also oxidative stress in exposed fish, but in the SOD enzyme, these changes were slightly higher in groups exposed to silver nitrate.

کلیدواژه‌ها [English]

  • Fish
  • Ionic and Silver Nanoparticles
  • Toxicology
  • Stress

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فصلنامه محیط زیست جانوری
دوره 11، شماره 1
اردیبهشت 1398
صفحه 239-246

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