نوع مقاله : ژنتیک
نویسندگان
1 گروه پاتوبیولوژی، دانشکدۀ دامپزشکی، دانشگاه تبریز، تبریز، ایران
2 گروه علوم پایه، دانشکدۀ دامپزشکی، دانشگاه تبریز، تبریز، ایران
3 گروه علوم درمانگاهی، دانشکدۀ دامپزشکی، دانشگاه تبریز، تبریز، ایران
4 مرکز تحقیقات بیماری های عفونی و گرمسیری، دانشکدۀ پزشکی، دانشگاه تبریز، تبریز، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Theileria species are obligatory intracellular hemoprotozoan parasites transmitted by various species of hard ticks and cause Theleriosis. Geographically, the disease has a global distribution and is often occurred in the tropical and subtropical areas of the world. In Iran, the small ruminant Theleriosis is caused by Theileria ovis and T. lestoquardi (Hirci). This study was aimed to identify Theileria ovis and T. lestoquardi among the small ruminants of East Azerbaijan province based on molecular method. For this purpose, a total of 166 whole blood samples (including 125 sheep and 41 goats) were collected from different regions of East Azerbaijan province, and then, all samples were examined under routine microscopic method and molecular assay. According to the molecular findings, 30(18%) of 166 specimens were only infected with T. ovis, whereas no infection was found on microscopic examination. The infection was only related to sheep samples. There was no significant difference between infection and age and sex, statistically. Sequencing showed that the amplified fragments in the present study was 98-100% identical to the corresponding sequences on GeneBank. Phylogenetic analysis indicated that the sequence obtained in this study with many sequences from different parts of the world in different years in a set and is closely related to these sequences. As a result, it can be noted that Theileriosis has a clinical and subclinical feature among the small ruminants of the East Azarbaijan province and T.ovis is the main cause of Theileriosis.
کلیدواژهها [English]