نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه محیط زیست، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس، نور، ایران
2 مرکز حفاظت فوک خزری ایران، جزیره آشوراده، بندرترکمن، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The study of museum specimens' DNA, is so important today because of the useful information it provides researchers. In addition, because of the genetic content for these samples is very fragile over time and sometimes low in their concentration, they need to more efficient methods with higher yields for optimal quantitative and qualitative extraction needed from the past. Therefore, depending on the target species or tissues, researchers often search to find a suitable way to achieve their goals by modifying and optimizing extraction protocols. In this study, by changing some parameters in the phenol chloroform method, the genetic content of 22 Caspian Seal samples containing fresh tissues and museum specimens was extracted. Also, the average of DNA concentration of fresh tissues and museum specimens according to the mean and standard error (M±SE) was 632.90±75.62 and 222.87±182.91 respectively and the mean of 260/280 wavelength ratio was closer to 1.8 nm in fresh tissue samples, then the DNA product of fresh muscle tissue samples had significantly higher concentration (P-value<0.05). The extracted DNA was amplified using two specific non-universal primers (Hg6.3 and Hg8.9) by polymerase chain reaction upon achievement to suitable temperature gradient. The results of molecular band documentation on 8% acrylamide gel indicated that all specimens, both fresh tissue and museum specimens with at least two decades, were successfully amplified. Given the ability of a successful genotype for population genetic studies, preservation of museum specimens under any circumstances is recommended.
کلیدواژهها [English]