نوع مقاله : سایر
نویسندگان
1 گروه ژنتیک و بیوتکنولوژی، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
2 گروه بیوشیمی بیوفیزیک، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Biofilms are one of the mechanisms which cause antibiotic resistance in Pseudomonas Aeruginosa. The aim of present study was to investigate the inhibitory effect of Iron oxide coated Chitosan nanoparticlses on Pseudomonas Aeruginosa clinical isolates and its relevance on bacterial biofilm. Fe3O4 nanoparticle powder synthesized by Co-precipitation method and modified with chitosan. Their properties determined by XRD, FTIR, DLS, TGA and SEM. After preparing the bacterial suspension, the biofilm formation measured in each untreated isolates. Then the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC( of iron oxide nanoparticles modified with chitosan which determined by microtiter plate assey. Synthesized nanoparticles inhibited the growth of Pseudomonas aeruginosa in various concentrations (from 0.512 ml/ml to 80mg/ml) also Minimum Inhibitory Concentration of nanoparticles had a direct relevance with the amount of bacterial biofilms. Based on biofilm inhibitory effect of ChitosanCoated Iron Oxide nanoparticles against Pseudomonas Aeruginosastrains, it seems that synthesized nanoparticles have got potential uses for pharmaceutical industries, but more studies are needed.
کلیدواژهها [English]