شناسایی مورفولوژیک و مولکولی گونه غالب خیار دریایی در منطقه اولی (خلیج فارس-استان بوشهر) و آنالیز برخی ترکیبات دیواره بدن آن

نوع مقاله: علوم جانوری

نویسندگان

1 گروه زیست شناسی دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر، ایران

2 پژوهشکده زیست فناوری دریا، مرکز تحقیقات زیست پزشکی شهدای خلیج فارس، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران

3 مرکز تحقیقات آبزیان خلیج فارس، دانشگاه خلیج فارس بوشهر، بوشهر، ایران

چکیده

هدف از این پژوهش، شناسایی یکی از گونه­ های غالب خیار دریایی در منطقه اولی- بوشهر ، با استفاده از روش مورفولوژیک و مولکولی و بررسی برخی ترکیبات تشکیل ­دهنده دیواره بدن ازجمله پروتئین کلاژن بوده است. تا علاوه بر شناسایی دقیق گونه، با مطالعه ترکیبات دیواره بدن آن بتوان، از یافته­ های این تحقیق در جهت ارتقاء صنایع غذایی و دارویی در کشور استفاده نمود. در این راستا، ابتدا با شناسایی مورفولوژیک از طریق بررسی اوسیکل­ های دیواره بدن و سپس با شناسایی مولکولی از طریق تعیین توالی بخشی از ژنوم میتوکندریایی 16SrRNA، گونه مورد بررسی به ­عنوان گونه Holothuria leucospilota شناسایی گردید. در بررسی ترکیبات دیواره بدن، پروتئین و رطوبت با مقادیر بالای 70% بیش ­ترین ترکیبات تشکیل ­دهنده بدن گونه مورد نظر بود و چربی کم ­ترین مقدار را در بین سایر ترکیبات داشته است. از بین ترکیبات پروتئینی، استخراج پروتئین کلاژن به ­روش هضم آنزیمی با پپسین صورت گرفت و با استفاده از آزمون SDS-PAGE، نوع آن و زیر واحدهای تشکیل ­دهنده آن با توجه به پروفایل وزن مولکولی، در مقایسه با سایر تحقیقات، صورت گرفت. روش استخراج موثر تشخیص داده شد و کلاژن استخراجی از نوع I بوده که دارای سه زنجیره 1α با وزن مولکولی حدود 140 کیلو دالتون می ­باشد.

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