مطالعه آسیب شناسی انگل Diclobothrium armatum در آبشش فیل ماهیان پرورشی در گیلان

نوع مقاله : بیماری ها

نویسندگان

1 گروه شیلات و آبزیان، واحد بهدان، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی گیلان، سازمان تحقیقات، آموزش و ترویج کشاورزی ایران، رشت، ایران

2 گروه ژنتیک، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران

3 انستیتو بین المللی ماهیان خاویاری، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران

چکیده

دیکلوبوتریوم آرماتوم نسبت به میزبان خود اختصاصی بوده و باعث آسیب­های مختلف شدید در آبشش، اختلال در سیستم تنفسی،کاهش اشتها، لاغری و تلفات مزمن در بچه ­ماهیان خاویاری می ­شوند. هدف از این مطالعه شناسایی آسیب­ های مختلف ناشی از تهاجم انگل در آبشش ماهیان خاویاری بوده است. در این تحقیق 40 قطعه از گونه فیل ­ماهی با وزن­ های 50 تا 150 گرم از اوائل خرداد ماه تا پایان شهریور 1397 به­ صورت تصادفی از مزارع پرورشی خاویاری صید گردیدند. ماهیان با همان شرایط زیستی به ­صورت زنده به آزمایشگاه بهداشت آبزیان انتقال داده شدند. معاینه بالینی آزمایشگاهی روی پوست، باله ­ها و تیغه ­های آبشش انجام گرفت. در آبشش ­ها پس از مشاهده انگل­ ها، آن­ ها را جداسازی کرده و جهت تثبیت در محلول فرمالین 4 درصد قرار داده شدند و سپس کار آبگیری، شفاف­ سازی و رنگ ­آمیزی آن­ ها با روش آلوم کارمین انجام گرفت.  هم ­زمان پس از جداسازی انگل­ ها، از بافت­ های آبشش نیز نمونه ­گیری کرده و جهت تثبیت در محلول بوئن انتقال داده شدند. آن­گاه پس از آبگیری، شفاف­ سازی، پارافینه کردن، برش دادن، چسباندن روی لام با روش هماتوکسیلین ائوزین رنگ­ آمیزی گردیدند. نتایج مشاهدات بالینی آبشش در ماهیان زنده: ترشحات موکوسی، چسبندگی در تیغه­ های آبشش و پرخونی را نشان می­ داد. نتایج مطالعات میکروسکوپی  در نمونه­ های بافت آبشش آلوده به انگل: چسبندگی در تیغه ­های آبشش ثانویه، دژنرسانس، ادم، نکروز، پرخونی، خونریزی، هیپرپلازی وتخریب بافت­ های آبشش را نشان داد.

کلیدواژه‌ها


عنوان مقاله [English]

Study of pathology of Diclobothrium armatum parasite in the gills of farmed Huso huso in Guilan

نویسندگان [English]

  • Mohammad Rahanandeh 1
  • Mahsa Rahanandeh 2
  • Ali Hallajian 3
  • Mehran Avakh keysami 1
1 Department of Fisheries and Aquaculture, Behdan Branch, Guilan Agricultural and Natural Resources Research and Training Center, Iran Agricultural Research, Education and Extension Organization, Rasht, Iran
2 Department of Genetics, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
3 International Sturgeon Institute, Agricultural Research, Education and Promotion Organization, Rasht, Iran
چکیده [English]

Diclobothrium armatum is  a host specific parasite , causing various severe damages to the gills , respiratory distress, loss of appetite, weight loss and chronic mortality in sturgeon. The purpose of this study was to identify the various damages caused by parasite invasion in gills of sturgeon. In this research 40 pieces of elephant species weighing 50 to 150 grams were harvested from sturgeon farms from early June until the end of September 2018. Fish were transferred to the aquatic animal health laboratory under the same biological conditions. Clinical laboratory examinations were performed on the skin, fins and gill tissue. They were also scientifically assisted to find out the causes of chronic deaths and the examination of the internal organs of young fish. After observing the parasites, they were isolated in the gills and placed in a 4% formalin solution, and the dehydration, clarification and coloring of them were carried out with Alum Carmine. At the same time, in order to gill pathology caused by parasites, after parasite separation, samples of gill tissue were also sampled and transferred to stabilize the Bowen solution. Then, after dehydration, clarification, paraffinization, cutting, and lamination, they were stained with Haematoxylin Eosin (H & E) method. The results of the clinical observations of the gill showed mucous secretions, gill tissue adhesions, and hyperemia.The results of microscopic studies in parasite-infected gill tissue samples: Adhesion in secondary gill lamela, degeneration, edema, necrosis, hyperemia, hemorrhage, hyperplasia, and destruction of gill tissue.
 

کلیدواژه‌ها [English]

  • Diclobothrium armatum
  • Gill
  • Huso huso
  • Pathology
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