تاثیر عصاره هیدروالکلی شقایق کوهی (Glaucium flavum) بر غلظت سرمی TSH در موش های صحرایی دیابتی شده با آلوکسان

نوع مقاله : بیماری ها

نویسندگان

1 دانشکده دامپزشکی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران

2 گروه علوم بالینی، دانشکده دامپزشکی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران

3 باشگاه پژوهشگران جوان و نخبگان، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران

4 دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

اختلال در ترشح TSH از غده هیپوفیز همواره یکی از عوارض بحث برانگیز دیابت بوده است. این مطالعه به ­منظور بررسی تاثیر عصاره خوراکی گیاه شقایق کوهی بر هورمون TSH در موش­های نر مبتلا به دیابت در مقایسه با موش­های سالم می‌باشد. در این مطالعه تجربی، 56 سر موش صحرایی نر بالغ نژاد ویستار به ­صورت تصادفی در 7 گروه 8 تایی تقسیم شدند: شاهد، سالم تیمار با عصاره شقایق کوهی به دوزهای 250 و 500 میلی‌ گرم بر کیلوگرم، شاهد دیابتی، دیابتی تیمار با عصاره به دوزهای 250 و 500 میلی‌ گرم بر کیلوگرم و دیابتی تیمار با داروی گلیبن‌کلامید با دوز 5 میکروگرم برکیلوگرم. دیابت با تزریق تک دوز آلوکسان القاء گردید. پس از گذشت 30 روز نمونه‌های خون جمع‌ آوری و غلظت TSH سرمی اندازه‌ گیری شد و در نهایت، داده‌ها با استفاده از آزمون آنالیز واریانس یک‌ طرفه مورد تجزیه و تحلیل آماری قرار گرفت. نتایج نشان می‌داد که میانگین گروه دیابتی+250 به‌ ­طور معنی‌ داری از گروه شاهد دیابتی بیش ­تر است (0/001>P)، هم‌ چنین میانگین‌ها در دو گروه دیابتی+500 و دیابتی+دارو فاقد رابطه معنی‌ دار با گروه شاهد بودند (0/05<P). جمع‌ بندی نتایج حاکی از آن است که، عصاره شقایق کوهی احتمالاً قادر به افزایش سطح TSH خون در موش‌ های دیابتی بوده به ­نحوی ­که در دوز بیش ­تر خود می‌ تواند این عارضه را تا سطح گروه شاهد بهبود بخشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Hydroalcoholic Extract of Glaucium flavum on Serum Level of TSH in Alloxan Induced Diabetic Rats

نویسندگان [English]

  • Ali reza Baneshi 1
  • Ardavan Nowroozi asl 2
  • Gholam Hossein Darya 3
  • Seyyedeh Maryam Mosavi 4
1 Faculty of Veterinary Medicine, Kazerun branch, Islamic Azad University, Kazerun, Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Kazerun branch, Islamic Azad University, Kazerun, Iran
3 Young Researchers and Elites Club Kazerun branch, Islamic Azad University, Kazerun, Iran
4 Faculty of Veterinary Medicine, Ahvaz Shahid Chamran University, Ahvaz, Iran
چکیده [English]

Disordered TSH secretion from pituitary gland has always been one of the controversial complications of diabetes. This study was conducted to evaluate the effect of oral administration of G. flavum on serum level of TSH in healthy and diabetic male rats. In this experimental study, 56 adult male Wistar rats were randomly divided into 7 groups of 8 including: healthy control, healthy treated with 250 and 500 mg/kg of G. flavum extract, diabetic control, diabetic treated with 250 and 500 mg/kg of the extract, and 5 µg/kg doses of Glibenclamide. Diabetes was induced by single injection of Alloxan. After 30 days, blood samples were collected and TSH levels were measured. Finally, the data were analyzed using one-way ANOVA. Results declare that, the mean of diabetic+250 group was significantly higher than diabetic control group (P<0.001); Also, the mean in diabetic+500 and diabetic+drug groups did not have a significant different with control group (P>0.05). In conclusion, The G. flavum extract have been able to increase the level of TSH in diabetic rats, somehow that the higher dose can improve the complication, as equal as healthy control group.

کلیدواژه‌ها [English]

  • Diabetes Mellitus
  • Glaucium flavum
  • TSH
  • Alloxan
  • Rat
  1. Ala, S.; Akha, O.; Kashi, Z.; Bahar, A.; Askari Rad, H.; Sasanpour, N. and Shiva, A., 2015. Changes in Serum TSH and T4 Levels after Switching the Levothyroxine Administration Time from before Breakfast to before Dinner. International Journal of Endocrinology. Vol. 15, pp: 1-5.
  2. Bercu, R.; Fãgãraş, M.; Jianu, L. and Dana, M., 2006. Anatomy of the endangered plant Glaucium flavum Cr., occurring on the Romanian Black Sea littoral Nature Conservation. pp: 273-280
  3. Boelen, A.; Wiersinga, W. and Fliers, E., 2008. Fasting-Induced Changes in the Hypothalamus–Pituitary–Thyroid Axis. Thyroid. Vol. 18, No. 2, pp: 123-129.
  4. Cabo, J.; Cabo, P.; Jimenez, J. and Zarzuelo, A., 1988. Glaucium flavum Crantz. Part v: Hypoglycemic activity of the aqueous extract. Phytotherapy Research. Vol. 2, No. 4, pp: 198-200.
  5. Chia, C.; Chena, W.; Chic, T.; Kuod, T.; Leeb, S. and Chenge, J., 2007. phosphatidylinositol -3- kinase is involved in the antihyperglycemic effect induced by resveratril in streptozotocin induced rats. Life Science. Vol. 80, pp: 1713-1720.
  6. Cortijo, J.; Villagrasa, V.; Pons, R.; Berto, L.; Martí Cabrera, M.; Martinez‐Losa, M. and Domenech, T., 1999. Bronchodilator and anti‐inflammatory activities of glaucine: In vitro studies in human airway smooth muscle and polymorphonuclear leukocytes. British journal of pharmacology. Vol. 127, No. 7, pp: 1641-1651.
  7. Dargan, P.; Button, J.; Hawkins, L.; Archer, J. R.; Ovaska, H.; Lidder, S. and Ramsey, J., 2008. Detection of the pharmaceutical agent glaucine as a recreational drug. European Journal of Clinical Pharmacology. Vol. 64, No. 5, pp: 553-554.
  8. Everson, A.; Nowak, J. and Thaddeus, S., 2002. Hypothalamic thyrotropin-releasing hormone mRNA responses to hypothyroxinemia induced by sleep deprivation. American Journal of Physiology-Endocrinology and Metabolism. Vol. 283, No. 1, pp: 85-93.
  9. Gardner, D.G. and Shoback, D.M., 2017. Greenspan's Basic and Clinical Endocrinology, 10th Ed. McGraw-Hill Education. pp: 681-683.
  10. GonzÁLez, C.; Montoya, E.; Jolin, T. and Gonzalez, 1980. Effect of Streptozotocin Diabetes on the Hypothalamic Pituitary-Thyroid Axis in the Rat. Endocrinology. Vol. 107, No. 6, pp: 2099-20103.
  11. Gurzov, E.; Stanley, j.; Pappas, G.; Thomas, E. and Gough, D. J., 2016. The JAK/STAT pathway in obesity and diabetes. The FEBS journal. Vol. 283, No. 16, pp: 3002-3015.
  12. Guyton M. and Hall, J.E., 2015. Guyton and Hall Textbook of Medical Physiology: Elsevier Health Sciences. pp: 912-915.
  13. Gyurkovska, V.; Philipov, S.; Kostova, N. and Ivanovska, N., 2015. Acetylated derivative of glaucine inhibits joint inflammation in collagenase-induced arthritis. Immunopharmacology and immunotoxicology. Vol. 37, No. 1, pp: 56-62.
  14. Hasani-Ranjbar, S.; Larijani, B. and Abdollahi, M., 2008. A systematic review of Iranian medicinal plants useful in diabetes mellitus. Archives of Medical Science. Vol. 4, No. 3, pp: 285.
  15. Kesavadev, J., 2017. Efficacy  and  safety  concerns regarding  complementary and  alternative     medicine  use  among  diabetes  patients. JPMA.  Vol. 67, pp: 316-319.
  16. Lenzen, S., 2008. The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia. Vol. 51, No. 2, pp: 216-26.
  17. Maratou, E.; Hadjidakis, D.; Peppa, M.; Alevizaki, M.; Tsegka, K.; Lambadiari, V. and Mitrou, P., 2010. Studies of insulin resistance in patients with clinical and subclinical hyperthyroidism. European journal of endocrinology. Vol. 163, No. 4, pp: 625-630.
  18. Nascimento-Saba, A.; Breitenbach, M.M.D. and Rosenthal, D., 1997. Pituitary-thyroid axis in short-and long-term experimental diabetes mellitus. Brazilian journal of medical and biological research. Vol. 30, pp: 269-274.
  19. Neag, M. A.; Mocan, A.; Echeverría, J.; Pop, R. M.; Bocsan, C. I.; Crişan, G. and Buzoianu, A. D., 2018. Berberine: Botanical Occurrence, Traditional Uses, Extraction Methods, and Relevance in Cardiovascular, Metabolic, Hepatic, and Renal Disorders. Frontiers in pharmacology. Vol. 9, pp: 557.
  20. Nikolova, T.; Berkov, S. H.; Doycheva, I. V.; Stoyanov, S. and Stanilova, I., 2018. GC/MS based metabolite profiling of five populations of Glaucium flavum (Ranunculales: Papaveraceae) from the Black Sea coast of Bulgaria. acta zoologica bulgarica. Vol. 11, pp: 91-94.
  21. Panda, S., 2008. The effect of Anethum graveolens L.(dill) on corticosteroid induced diabetes mellitus: involvement of thyroid hormones. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. Vol. 22, No. 12, pp: 1695-1697.
  22. Panveloski-Costa, A.C.; Silva Teixeira, S.; Ribeiro, I.M.R.; Serrano-Nascimento, C.; Das Neves, R.X.; Favaro, R.R.; Seelaender M.; Antune, V. and Nunes, M., 2016. Thyroid hormone reduces inflammatory cytokines improving glycaemia control in alloxan-induced diabetic wistar rats. Acta Physiologica. Vol. 217, No. 2, pp: 130-140.
  23. Rondeel, J.M.; de Greef, W.J.; Heide, R. and Visser, T.J., 1992. Hypothalamo-hypophysial-thyroid axis in streptozotocin-induced diabetes. Endocrinology.  Vol. 130, No. 1, pp: 216-220.
  24. Štrbák, V., 2018. Pancreatic Thyrotropin Releasing Hormone and Mechanism of Insulin Secretion. Cellular Physiology and Biochemistry. Vol. 50, No.1, pp: 378-384.
  25. Subramoniam, A., 2016. Plants with Anti-Diabetes Mellitus Properties: CRC Press.
  26. Yoshida, S.; Kobayashi, Y.; Nakama, T.; Zhou, Y.; Ishikawa, K.; Arita, R.; Miazaki, M.; Sassa, Y. and Oshima, Y., 2015. Increased expression of M-CSF and IL-13 in vitreous of patients with proliferative diabetic retinopathy: implications for M2 macrophage-involving fibrovascular membrane formation. British Journal of Ophthalmology. Vol. 99, No. 5, pp: 629-634.
  27. Zoeller, R.T.; Tan, S. and Tyl, R.W., 2007. General background on the hypothalamic-pituitary-thyroid (HPT) axis. Critical reviews in toxicology. Vol. 37, No. 12, pp: 11-53.