تاثیر محرک ایمنی ماکروگارد و جلبک اسپیرولینا (Arthrospira platensis) بر برخی فراسنجه‌های خونی ماهی ازون برون (Acipenser stellatus)‎

نوع مقاله : تغذیه

نویسندگان

1 گروه شیلات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه بهداشت و بیماری‌های آبزیان، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

چکیده

اثرات محرک ایمنی ماکروگارد و اسپیرولینا بر برخی پارامترهای خونی ماهی ازون ­برون جوان در این مطالعه مورد سنجش قرار گرفت. این تحقیق با استفاده از یک طرح کاملاً تصادفی با 5 تیمار و 3 تکرار، شامل تیمار شاهد (M0S0) که واجد جیره غذایی پایه بدون افزودنی، جیره پایه مکمل شده با 0/1 درصد ماکروگارد (M1S0)، جیره پایه مکمل شده با 0/1 درصد پودر اسپیرولینا (M0S1)، جیره پایه مکمل شده با 0/1 درصد ماکروگارد به ­همراه 0/1 درصد پودر اسپیرولینا (M1S1) و جیره پایه مکمل شده با 0/5 درصد ماکروگارد به­ همراه 0/5 درصد پودر اسپیرولینا (M5S5) طراحی شد. تعداد 700 عدد ماهیان ازون ­برون (وزن متوسط ​​1/06±87/56 گرم) به ­مدت 12 هفته با جیره‌های آزمایشی تغذیه شد. در انتهای آزمایش، تعداد گلبول‌های سفید (WBC)، تعداد گلبول‌های قرمز (RBC هماتوکریت (HCT)، میزان رسوب گلبول‌های قرمز (ESR) و شمارش افتراقی گلبول‌های سفید مورد سنجش قرارگرفت. حداکثر مقادیر WBC و HCT در تیمار M5S5 بود، درحالی‌که حداقل مقدار ESR در این تیمار مشاهده شد (0/05>p). میزان شاخص RBC در تیمار M5S5 نسبت به تیمار شاهد افزایش یافت. حداکثر مقدار جمعیت لنفوسیتی (2/10±75/08 درصد) در تیمار M5S5 مشاهده شد و حداکثر مقدار نوتروفیل‌ها در تیمارهای M5S5 و M1S1 مشاهده شد (0/05<p). بنابراین، استفاده توأم از ماکروگارد و اسپیرولینا هرکدام در سطح 0/5 درصد در جیره غذایی ماهی ازون برون به‌طور معنی‌داری سبب بهبود شاخص‌های خونی، جمعیت لنفوسیت‌ها و نوتروفیل‌ها می‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of immune motivator Macrogard and Spirulina (Arthrospira platensis) on some hematological parameters of stellate sturgeon (Acipenser stellatus)

نویسندگان [English]

  • Ammar Salehi Farsani 1
  • Mehdi Soltani 2
  • Abolghasem Kamali 1
  • Mehdi Shamsaie Mehrgan 1
1 Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Aquatic Animal Health, Veterinary Faculty, University of Tehran, Tehran, Iran
چکیده [English]

This study was carried out to evaluate the effects of Macrogard and Spirulina on some hematological parameters of juvenile farm-raised stellate sturgeon. This study was designed using a completely random plan containing 0% (control), 0.1% Spirulina (M0S1), 0.1% Macrogard (M1S0), 0.1% Macrogard and 0.1% Spirulina (M1S1) and 0.5% Macrogard and 0.5% Spirulina (M5S5) in 5 treatments with 3 repetitions. A total number of 700 stellate sturgeon fish (average weight of 87.56±1.06 g) were fed during 12 weeks. At the end of trial, the blood samples were collected and haematological parameters including leucocyte count (WBC), erythrocyte count (RBC), haematocrit (HCT), erythrocyte sedimentation rate (ESR) and the white blood cell differential were measured. The maximum level of WBC and HCT were observed in M5S5, while the lowest level of ESR was seen in M5S5 (p<0.05). The population size of RBC was significantly higher than control group. The highest population size of lymphocyte (75.08+2.10%) was measured in M5S5 and the also highest population size of neutrophil was observed in M1S1 and M5S5 (p<0.05). Therefore, using Macrogard and spirulina, each at 0.5% in stellate sturgeon diet, significantly hematological indices, population size lymphocytes and neutrophils.

کلیدواژه‌ها [English]

  • Stellate sturgeon
  • Spirulina
  • Macrogard
  • Hematological indices
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