نوع مقاله : بیماری ها
نویسندگان
1 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
3 جهاد دانشگاهی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
In this study efficacy and immunogenicity of Streptococosis/lactococosis vaccine administrated via oral, bath and i.p routs were investigated. 600 fish (14±2.1g) were randomly divided i 4 similar groups in triplicate as follows: group1 was orally vaccinated with 108 cfu/g food for two weeks. Group2: were injected with 0.1ml of 109 cfu/ml bacterins. Group 3 was vaccinated via immersion rout for 2 minutes in 109 cfu/ml. Group 4: none immunized group without vaccination. Fishes were reared for 2 month in similar situation and blood samples were taken from each group at days zero, 20, 40 and 60 of experiment. Immunological parameters including: antibody titer, serum lysozyme, complement, bactericidal activity and hematological parameters were compared among the groups. Fishes were challenged with S.iniae and L.garviae separately in the end of experiment and rate of mortality compared among the groups. Result showed that rate of mortality in challenged fish with S.iniae in injection, oral, immersion and unvaccinated routs were 20±10, 56.3±10, 50±5.67 and 90± 3.67 whereas in challenged fish with L.garviae were 26.67±5.77, 57.7±10, 43.33±5.67 and 90± 4.63 respectively. Immunological parameters were increased significantly just in injection vaccine treatment increased in sampling periods (P<0.05). it can be concluded that Although injection rout cause the best protection and immunogenicity, oral and immersion routs induce relative protection without notable immunogenicity in rainbow trout and each rout can be used based on farm situation.
کلیدواژهها [English]