نوع مقاله : ژنتیک
نویسندگان
1 دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران, دانشکده علوم و فنون دریایی, گروه بیولوژی دریا, ایران
2 انستیتو پاستور ایران, مرکز تحقیقات بیوتکنولوژی, آزمایشگاه ونوم و توکسین, ایران
3 دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران, دانشکده علوم و فنون دریایی, گروه شیمی دریا, ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Identification of venomous species of Persian Gulf cone snails and characterization of venom composition and their features is so important from the point of danger for underwater divers as well as medical importance. In this research, the specimens of Conus textile were collected off Larak Island from depth of 7 m. The collected samples were transferred to laboratory alive and were stored at -700C. The venom´s ducts were separated and homogenized with deionized water. The mixture centrifuged at 13000 rpm for 15 minutes at 4 ̊C. Supernatant was considered as extracted venom and stored at -20 C after lyophylization. The entity of venom determined by SDS-PAGE and RP-HPLC methods used to investigate the extracted venom and system to determine protein pattern. The results obtained with SDS-PAGE showed that proteins and peptides of venom were ranged between 6 to 250KD. Chromatogram of the venom demonstrated more than 44 large and small fractions.
کلیدواژهها [English]