Effect of different wheat varieties on α-amylase enzyme activity in midgut of Mediterranean flour moth Anagasta kuehniella (Lep. Pyralidae) and its fecundity

Document Type : Biology(Animal)

Authors

1 Faculty of Agriculture, Vali-asr University of Rafsanjan, Rafsanjan, Iran

2 Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Abstract

Mediterranean flour moth Anagasta kuehniella (Lep. Pyralidae) is a major pest of stored products which causes a lot of damage on stored products, especially flour and grains. Cereals which are rich in carbohydrates especially starch are the most important food source for flour moth. Thus the survival of the insect is dependent on alphaamylase enzyme activity that converts starch to simply-absorbed sugars. In this study alpha-amylase enzyme activity and pest fecundity were examined on ten wheat varieties. Fecundity rates were significant in different varieties. There was positive correlation between Fecundity rates and protein rates in different wheat varieties (r= 0.461). The activity of alpha-amylase enzyme in midgut of fifth instars larvae on different wheat varieties and inhibitory rate of different wheat extracts on the alpha-amylase enzyme activity in both sexes was significantly different. Most of wheat varieties had high inhibitory activity in both sexes. A negative correlation observed between alpha-amylase enzyme activity and Protein amount of different wheat varieties (r=-0.582 male, r=-0.453 female). The results showed that there was no correlation between inhibition of alpha-amylase activity and the fecundity average of females (r= 0.045) in different varieties of wheat. 

Keywords