Nucleotide variation study of wild sheep populations of the Tangsayad and Korayi guardianship regions using D-loop region and Cyt-b gene of mitochondrial genome

Document Type : Genetic

Authors

1 Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University, Varamin Branch - Pishva

2 Department of Genetic and Biotechnology, Faculty of Biological Sciences,Varamin-Pishva Branch, Islamic Azad University, Pishva

3 Environmental Protection Agency, Tehran, Iran

Abstract

In order to study of nucleotide variation of wild sheep populations of Tangsayad and Korayi guardianship regions, individual feces were taken from two populations. Then, in order to genetic comparison between two populations using D-loop region and Cyt-b gene of mitochondrial genome, DNA was extracted. After amplifying by PCR, the samples were sequenced. Then, sequences were analyzed using MEGA4 software. Results indicated that D-loop region has 552 base pair and Cyt-b gene has 589 base pair and no significant genetic variation was observed between the two populations.

Keywords

Journal of Animal Environment
Volume 2, Issue 4
February 2010
Pages 25-30

Files

Share

How to cite

Statistics