Study on molecular genetic of Crocodylus palustris population in Sistan & Baluchistan province

Document Type : Genetic

Authors

Department of Animal Sciences, Faculty of Agriculture, Islamic Azad University, Varamin Pishva Branch, Iran

Abstract

For the first time, study on Crocodylus palustris population was conducted in Sistan & Baluchistan province of Iran for the first time. They distribution is being limited to part of Makran in Baluchistan and also part of Yahoukalat region. The Crocodylus palustris has a medium or big body with wide snout. In Iran, the population of this animal belongs to small number of them in whole world. Sample was made from 12 crocodiles captive in Dargas region of Chabahar province and after biometric measurement, the samples was provided from scale tissue and then DNA was extracted by kit. RAPD seven primers with 10 Nucleotides sequence was used for RAPD-PCR. All seven primers except primers number 3 caused polymorphism among samples. Averagely, in per use of these primers, seven locus are present polymorphism. The result was analyzed by PopGene32 software which showed approximately 56% of polymorphism was among samples and genetic species in this population.

Keywords