اثرات به‌کارگیری سوربات پتاسیم در جیره بیان ژن های مرتبط با رشد در ماهی کپورمعمولی (Cyprius carpio)

نوع مقاله : علوم جانوری

نویسندگان

گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

این تحقیق با هدف بررسی تأثیر سطوح مختلف نمک سوربات پتاسیم در جیره بر بیان ژن­ های مرتبط با رشد بچه ­­ماهیان کپور معمولی صورت پذیرفت. بدین ­منظور تعداد 168 قطعه ماهی کپور با میانگین وزنی 2/6±15/2 گرم به ­مدت 8 هفته، در چهار تیمار و سه تکرار با جیره ­های آزمایشی حاوی سطوح مختلف صفر (شاهد)، 0/5، 1 و 2 درصد سوربات پتاسیم تغذیه شدند. در پایان دوره از کبد و مغز نمونه ­برداری، استخراج RNA انجام و برای سنتز cDNA از کیت SuPrime Script RTase استفاده ‌شد.cDNA  حاصله با استفاده از پرایمرهای ژن­ های مرتبط با رشد (IGF1 و GH) و ژن بتا­اکتین به ­عنوان ژن رفرنس در Real Time PCR استفاده شد. بیان هر دوژن در تیمارهای تغذیه شده نسبت به تیمار شاهد افزایش یافت. اختلاف معنی‌ داری در میزان بیان ژن GH در تیمارهای تغذیه‌ شده با 1 و 2 درصد نسبت به تیمار 0/5 درصد مشاهده شد (0/05>P). بیان ژن IGF-1 فقط در تیمار 1 درصد اختلاف معنی­ دار با تیمار 0/5 درصد نشان داد (0/05>P). نتایج نشان داد که بهترین عملکرد بیان ژن رشد مربوط به تیمارهای تغذیه ­شده با جیره حاوی 1 درصد سوربات پتاسیم می­باشد و می­ تواند به­ عنوان محرک رشد در جیره غذایی آبزیان استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effects of dietary administration of Potassium Sorbate on growth related genes expression in common carp (Cyprinus carpio)

نویسندگان [English]

  • Niloofar Molaee ghasemi
  • Ali Shabany
  • Roghieh Safari
Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

The present study was performed to investigate the effect of different levels of Potassium Sorbateon expression of genes related to growth (GH and IGF1) in Cyprinus carpio. For this purpose, 168 fish with average weight of 15.2 ± 2.6 g were fed on diets supplemented with 0%, 0.5%, 1% and 2% Potassium Sorbate(PS) for 8 weeks. At the end of feeding trial, RNA extracted from liver and brain, cDNA syntesised with SuPrime Script RTase and Real-time PCR analysis performed by using primers relate to GH and IGF1, and Beta-actin as housekeeping genes. Expression of both growth genes increased in fish fed supplemented diet compare to control. Significant differences (P<0.05) were observed in expression of GH in fish fed 1% and 2% PS when compared to 0.5% and control. The expression of IGF1 gene showed significant difference in 1% PS treatment compared 0.5% (P<0.05). These results revealed beneficial effects of 1% dietary PS on growth related genes expression in Common carp and can be used as a growth stimulant in aquatic diets.

کلیدواژه‌ها [English]

  • Growth
  • common carp
  • Potassium Sorbate
  • Gene expression

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فصلنامه محیط زیست جانوری
دوره 11، شماره 4
دی 1398
صفحه 223-228

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