بررسی میزان رشد و پروفایل اسیدهای چرب ریزجلبک Chlorella vulgaris در محیط کشت حاوی فاضلاب شهری

نوع مقاله : بوم شناسی

نویسندگان

1 گروه شیلات، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

2 گروه زیست شناسی دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران

چکیده

 این تحقیق به ­منظور تعیین محتوای اسیدهای چرب در ریزجلبک Chlorella vulgaris کشت شده در تیمارهای مختلف فاضلاب شهری انجام گردید. چهار تیمار (هر تیمار با 3 تکرار) با درصدهای مختلف فاضلاب شهری و محیط کشت f/2 تهیه و در شرایط آزمایشگاهی نگه ­داری شدند. جداسازی ریزجلبک در مراحل مختلف آزمایش با استفاده از دستگاه سانتریفوژ انجام گردید. میزان رشد و اسیدهای چرب در همه تیمارها اندازه­ گیری شد. نتایج نشان داد که طی دوره کشت، تیمارهای 1 و 4 بیش ­ترین رشد را داشتند. بین همه تیمارها بیش ­ترین مقدار اسیدهای چرب اشباع مربوط به C17:0 (هپتادکانوئیک اسید) و C6:0 (هگزانوئیک اسید) بود و هم ­چنین بیش ­ترین مقدار اسیدهای چرب غیراشباع با یک پیوند دوگانه مربوط به C16:1 (پالمیتولئیک اسید) و C18:1Cis9 (اولئیک اسید) بود. از نظر مقادیر اسیدهای چرب غیراشباع تک پیوندی (MUFA) و چندپیوندی (PUFA) بین تیمارها اختلاف معنی ­دار مشاهده شد (0/05>p). نتایج آنالیز واریانس یک ­طرفه (One-Way ANOVA) در تاثیر محیط­ های کشت بر مقادیر اسید چرب امگا 3 و 6 تفاوت معنی ­داری را بین تیمارها نشان داد (0/05>p). بیش ­ترین مقدار مربوط به اسید چرب امگا 3 در تیمار 2 (18/28 درصد) بود، درصورتی ­که در سایر تیمارها مقدار این اسید کم ­تر از 2 درصد ثبت گردید. بیش ­ترین مقدار امگا 6 هم در تیمار 2 و 4 با مقدار تقریبی 12 درصد اندازه ­گیری شد. براساس نتایج تحقیق حاضر پیشنهاد می­ شود که از فاضلاب به ­عنوان محیط کشت جایگزین برای تکثیر و پرورش انبوه ریزجلبک Chlorella vulgaris به ­عنوان منبعی غنی از امگا 3 استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of the Growth rate and Fatty acids profile of microalgae Chlorella vulgaris reared in culture medium containing urban sewage

نویسندگان [English]

  • Javad Ghasemzadeh 1
  • Fatemeh Lavajoo Balgoori 2
  • Mansoor Barazpoor 1
1 Department of Fisheries, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
2 Department of Marine Biology, Faculty of Marine Sciences, Hormozgan University, Bandar Abbas, Iran
چکیده [English]

This research aimed to measure the fatty acids content of Cholorella vulgaris reared in different culture media containing urban sewage. Four treatments (each with three replicates) containing different ratios of urban sewage and f/2 culture medium were prepared,and kept in the laboratory condition. The microalgae cells were separated by centrifugation at different stages of the experiment. The growth rate and fatty acids profile of all treatments were measured. The results showed that treatments 1 and 4 had the highest growth rate. Among all the treatments the highest fatty acid content were those of 0:C17 (Heptadecanoic acid) and 0:C6 (Hexanoic acid), and the highest amount of unsaturated fatty acids with a single double bond belonged to 1:C16 (Palmitoleic acid) and C18:1Cis9 (Oleic acid). Significant differences (p<0/05) were observed between the monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) of all treatments. The results of ANOVA on the effects of the culture medium on the Omega-3 (ω−3) and Omega-6 (ω−3) content revealed significant differences (p<0/05) between all treatments. The highest ω−3 content was recorded in treatment 2 (18.28%), whereas in the other treatments it was less than 2%. The highest value of ω−6 was recorded in treatments 2 and 4. Based on the results of this research, we recommend that urban sewage can be utilized as a substitute culture medium for the growth and mass production of C. vulgaris microalgae as a rich sources of fatty acids especially ω−3.

کلیدواژه‌ها [English]

  • Chlorella vulgaris
  • urban sewage
  • growth
  • ω−3 and ω−6 fatty acids

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فصلنامه محیط زیست جانوری
دوره 12، شماره 1
فروردین 1399
صفحه 401-408

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