اثر حفاظتی عصاره هیدروالکلی میوه تمشک (Rubus fruticosus L) بر سطح سرمی لیپیدهای خون در موش های صحرایی نر دیابتیک القا شده با STZ

نوع مقاله : فیزیولوژی (جانوری)

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

2 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ایران

3 گروه بیوشیمی، دانشکده پزشکی، دانشگاه علوم پزشکی و خدمات درمانی همدان، همدان، ایران

چکیده

 کاهش میزان گلوکز و لیپیدهای نامطلوب سرم در بیماران دیابتی دارای اهمیت است. دراین مطالعه تاثیر عصاره هیدروالکلی میوه تمشک (Rubus fruticosus L) و میزان لیپیدهای خون در رت­ های دیابتی شده با استرپتوزوتوسین مورد بررسی قرار گرفت. 50 سر موش نر نژاد ویستار با وزن 20±200 گرم به 5  گروه مساوی تقسیم شدند. گروه شاهد ، گروه شاهد دیابتی شده و 3 گروه تجربی دیابتی شده که عصاره هیدروالکلی میوه تمشک را با دوزهای 50 میلی­ گرم/کیلوگرم، 100 میلی ­گرم/کیلوگرم و 200 میلی­ گرم/کیلوگرم دریافت کردند. برای القا دیابت استرپتوزوتوسین (55 میلی­ گرم/کیلوگرم) به ­صورت ip تزریق و بعد از 4 هفته نمونه خونی تهیه شد و داده­ ها با نرم ­افزار spss مورد تحلیل آماری قرار گرفت. سطح سرمی کلسترول تام،LDL  و تری ­گلیسرید سرم در گروه دیابتی نسبت به گروه شاهد افزایش معنی­ داری یافت (p<0.05),(P<0/01) و (P<0/001). اما سطح سرمی HDL در گروه  دیابتی نسبت به گروه شاهد کاهش داشت (P<0/05) و سطح سرمی کلسترول تام،  LDL و تری­ گلیسیرید  در گروه  دیابتی دریافت­ کننده عصاره هیدروالکلی تمشک کاهش معنی ­داری نسبت به گروه دیابتی داشته است  (p<0.05),(P<0/01) و (P<0/001). ­سطح سرمی HDL در گروه دیابتی دریافت­ کننده عصاره هیدروالکلی تمشک افزایش معنی ­داری نسبـت به گروه دیابتی نشان داد (0/05>P) و (0/01>P). دیابت موجب افزایش چربی­ های خطر­ساز در سرم خون رت ­ها می­ گردد. عصاره هیدروالکلی تمشک به ­دلیل دارا بودن ترکیبات آنتی ­اکسیدانی اثرات سودمندی بر لیپیدهای نامطلوب سرم دارد. 

کلیدواژه‌ها

عنوان مقاله [English]

Protective effect of hydroalcoholic extract of Raspberry fruit (Rubus fruticosus L.) on serum lipid profile in STZ-induced diabetic male rats

نویسندگان [English]

  • Mahya Soheilifar 1
  • Abdolhossein Shiravi 1
  • Naser Mirazi 2
  • Vida Hojati 1
  • Roghayeh Abbasaliepourkabireh 3
1 Department of Biology, Faculty of Basic Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran
2 Department of Biology, Faculty of Basic Sciences, Bu Ali Sina University, Hamadan, Iran
3 Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences and Health Services, Hamadan, Iran
چکیده [English]

The effect of hydroalcoholic extract of Raspberry fruit (Rubus fruticosus L.) on serum lipids levels in streptozotocin-induced diabetic rats was investigated in this study. 50 male Wistar rats weighing 20 ± 200g were divided into 5 groups. The non-diabetic control group, the diabetic control group and the three diabetic experimental groups received the hydroalcoholic extract of Raspberry fruit at doses of 50, 100 and 200 mg / kg. Diabetes was induced by an intraperitoneal injection of STZ (55 mg / kg). After 4 weeks serum samples were collected from all groups and serum lipids were measured. Data were analyzed using SPSS. Results: The results showed that serum levels of total cholesterol, LDL and triglyceride significantly increased in diabetic group compared to control group (P <0.001), (P <0.05), (P <0.01). While serum HDL level in the diabetic group was lower than the control group (P <0.05). Moreover, serum levels of total cholesterol, LDL and triglyceride significantly decreased in the diabetic group receiving the hydroalcoholic extract of Raspberry (p <0.05), (P <0.01) and (P <0/001) respectively. The serum HDL level in the diabetic group receiving the raspberry hydroalcoholic extract showed a significant increase in comparison with the diabetic group (P <0.05), (P <0.01).
Results of this research showed that diabetes increases the risk of undesirable lipids in the blood serum of rats. Hydroalcoholic extract of raspberries has beneficial effects on reducing lipids due to antioxidant activity.

کلیدواژه‌ها [English]

  • Raspberry
  • Diabetes
  • Rat
  • Serum lipids

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فصلنامه محیط زیست جانوری
دوره 12، شماره 2
تیر 1399
صفحه 61-66

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