بررسی اثرات مواجهه کوتاه‌مدت شرایط تنش حرارتی-رطوبتی بر رشد و نمو جنین در موش های آزمایشگاهی باردار

نوع مقاله : زیست شناسی (جانوری)

نویسندگان

1 گروه اقلیم‌شناسی، دانشکده جغرافیا و علوم محیطی، دانشگاه حکیم سبزواری، سبزوار، ایران

2 گروه زیست‌شناسی، دانشکده علوم پایه،دانشگاه حکیم سبزواری، سبزوار، ایران

3 گروه علوم و مهندسی محیط‌زیست، دانشکده جغرافیا و علوم محیطی، دانشگاه حکیم سبزواری، سبزوار، ایران

چکیده

تنش ‌های حرارتی-رطوبتی از مهم ­ترین عوامل محیطی موثر بر رشد جنین و سلامت مادران باردار است و مطالعات در این زمینه محدود بوده‌ اند. هدف این پژوهش بررسی اثرات مواجهه کوتاه‌ مدت در برابر تنش‌ های حرارتی-رطوبتی در قالب شاخص THI بر رشد و نمو جنین در موش می ‌باشد و به این منظور از مدل ­های حیوانی (موش NMRI) استفاده شده است. در مطالعه حاضر موش ‌های NMRI با سن 7 تا 8 هفته باردار در طول دوره دوم بارداری استفاده شده است. روزانه به­ صورت کوتاه ‌مدت (3، 5، 8، 10 و 15 دقیقه) و در بازه زمانی 1 هفته در برابر تنش‌های حرارتی-رطوبتی در محفظه طراحی شده قرار گرفتند. دوره ­های قرارگیری مطابق با نقاط رشد و تکامل جنین انسان طراحی شده است. مواجهه با تنش‌ های حرارتی-رطوبتی در دوره دوم (روز بارداری: 14-6/5) بارداری در مدت زمان 7 روز، افزایش میزان مرگ و میر مادران، کاهش وزن قد جنین و وزن جفت را نسبت به گروه شاهد نشان می ‌دهد، این افزایش در سطح معنی‌داری (0/01 >p-value) مورد آزمون قرار گرفت. هم­ چنین با افزایش سطح تنش (حرارت-رطوبتی) در سطوح مختلف شاخص، ناهنجاری­ های جنینی (اگزوهپاتیک، بدشکلی صورت، بدن C شکل، نقایص ساختاری گوش، همانژیوم و چسبندگی اندام حرکتی) افزایش نشان داده است. آزمون t دو نمونه مستقل و ANOVA معنی ­داری موارد فوق را در سطح (0/01 >p-value) تایید نموده‌ اند. نتایج این پژوهش می ‌تواند در تدوین برنامه ‌های مراقبتی، درمانی و آموزش مادران باردار موثر بوده و جهت پایش تنش‌ های حرارتی-رطوبتی و طراحی سیستم‌ های هشدار دهنده مورد استفاده قرار گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Identify the short-term effects of exposure thermos-hygrometric stress on embryonic development

نویسندگان [English]

  • Fatemeh Mayvaneh 1
  • Alireza Entezari 1
  • Fatemeh Sadeghifar 2
  • Mohammad Baaghideh 1
  • Azadeh Atabati 3
1 Department of Climatology, Faculty of Geography and Environmental Sciences, Hakim Sabzevari University, Sabzevar, Iran
2 Department of Biology, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar, Iran
3 Department of Environmental Science and Engineering, Faculty of Geography and Environmental Sciences, Hakim Sabzevari University, Sabzevar, Iran
چکیده [English]

Heat-humidity stress is one of the most important environmental factors affecting the development of the fetus and the health of pregnant maternal., Studies in this area have been limited. The aim of this study was to investigate the effects of short-term exposure to heat-humidity stress in the form of THI on fetal growth and development. For this purpose, animal models were used...  pregnant NMRI mice that were exposed to heat-humidity stress in the chamber during the second period of pregnancy (3, 5, 8, 10 and 15 minutes) daily for short periods Placement courses (GD 14.5-17) is designed in accordance with the growth and development of the human embryo. Exposure to thermal-humidity stresses in the second period (GD6.5-14) during of pregnancy shows an increase in maternal mortality and fetal weight loss, crown-to-rump length (CLR) and placental weight in comparison with the control group. Also, with increasing thresholds of stress at different thresholds of the index, embryonic abnormality (Exohatics, Facial Deformity, C-Shaped Body, Ear structure defects, Hemangioma and Limbic Adhesion) have increased. T-test of two independent samples and ANOVA confirmed the above-mentioned results at the level (p-value<0.01). The results of this study can be effective in the development of care programs, treatment and education of pregnant mothers, and to monitor the thermal stresses and design of warning systems.

کلیدواژه‌ها [English]

  • Heat-Humidity stress
  • Fetal Abnormalities
  • THI
  • Exposure

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فصلنامه محیط زیست جانوری
دوره 12، شماره 3
مهر 1399
صفحه 69-76

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