تاثیر غلظت های مختلف دی فرمات سدیم بر شاخص های بیوشیمیایی و آنتی اکسیدانی سرم در ماهی آزاد دریای خزر (Salmo trutta caspius)

نوع مقاله: بیماری ها

نویسندگان

1 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

10.22034/aej.2020.117621

چکیده

ماهی آزاد دریای خزر  (Salmo trutta caspiusیکی از 9 زیرگونه قزل­ آلای قهوه ­ای در جهان و از گونه­ های بومی ایران می­ باشد که در معرض خطر انقراض می­ باشد. هدف از این تحقیق بررسیاثر غلظت ­های مختلف اسیدی­ فایر دی فرمات سدیم بر روی شاخص ­های بیوشیمیایی و آنتی ­اکسیدانی سرم در ماهی آزاد دریای خزر بود. بدین ­منظور، تعداد 360 قطعه بچه ­ماهی آزاد دریای خزر با وزن حدود 0/05±13 گرم به ­مدت 60 روز  با به ­کارگیری چهار جیره شامل سطوح صفر (شاهد)، 0/5، 1 و 1/5 درصد، از دی فرمات سدیم در 4 تیمار و 3 تکرار مورد بررسی قرار گرفتند. خونگیری از ماهیان در شروع آزمایش، روز 30 و پس از پایان دوره از قسمت ساقه دمی، انجام شد. در هر بار نمونه ­برداری بعد از خونگیری فعالیت آنزیم ­های کاتالاز، سوپراکسید دیسموتاز (SOD) و مالون دی آلدهید (MDA) و هم ­چنین میزان تری­ گلیسرید، کلسترول، لاکتات دی هیدروژناز LDH و گلوکز توسط روش ­های بیوشیمیایی تعیین شد. نتایج نشان داد که مقدار گلوکز در 30 روز ابتدایی آزمایش در همه تیمار­ها اختلاف معنی­ داری نسبت به گروه شاهد داشته است. مقدارکلسترول سرم خون در روز 30 در تیمار 0/5 درصد و در روز 60 در تیمار 1 درصد نسبت به گروه شاهد کاهش معنی ­داری داشته است. مقدار تری­ گلیسرید در تمام گروه ­ها به ­جز تیمار 1 درصد در انتهای دوره افزایش معنی داری نسبت به شروع آزمایش داشته است. هم ­چنین در تیمار 0/5 و 1/5 درصد در روز صفر و 30 با روز 60 اختلاف معنی ­داری مشاهده شد. میزان آنزیم LDH  در روز 30 نمونه ­برداری، در تیمار 1 درصد کاهش معنی­ داری نسبت به گروه شاهد داشته است. در مورد میزان کاتالاز سرم در روز 60آزمایش اختلاف معنی ­داری میان تیمارهای اسیدی ­فایر با گروه شاهد مشاهده نشد. میزان SOD پس از 60 روز نسبت به گروه شاهد معنی ­دار نبوده است. میزان فعالیت مالون دی آلدهید در تیمار 1 درصد در روز 30 و 60 نمونه­ برداری و تیمار 1/5 درصد در روز 60، اختلاف معنی­ داری با گروه شاهد داشتند. نتایج این تحقیق نشان می ­دهد که تیمارهای اسیدی­ فایر به خصوص تیمار 1 و 1/5 درصد تاثیر معنی­ دار در فاکتور بیوشیمایی و دفاع آنتی ­اکسیدانی ماهی آزاد تا 30 روز اول از خود نشان داده ­اند اما در 30 روز دوم ازمایش نتایج برعکس بود.

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