تاثیر عصاره الکلی گیاه زنیان (Trachyspermum ammi L) بر عملکرد رشد، فراسنجه های خونی و ایمنی ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss)

نوع مقاله: تغذیه

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

2 گروه بهداشت آبزیان، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

10.22034/aej.2020.118783

چکیده

در این تحقیق تاثیر عصاره گیاه زنیان (Trachyspermum ammi L) بر شاخص‌ های رشد و ایمنی ماهی قزل ­آلای رنگین ­کمان (Oncorhynchus mykiss) مورد بررسی قرار گرفت. در این بررسی 600 قطعه ماهی قزل ­آلا با میانگین وزنی اولیه 7±58 با جیره‌ های غذایی به ­ترتیب حاوی 0/5، 1/5 و 2/5 درصد عصاره زنیان و گروه شاهد با جیره فاقد عصاره تغذیه گردیدند. در پایان دوره، شاخص ‌های رشد، خون­ شناسی و ایمنی براساس فرمول‌ های استاندارد محاسبه و با استفاده از آزمون توکی آنالیز انجام گردید. در گروه ماهیانی که 2/5 درصد عصاره زنیان دریافت کرده بودند شاخص ‌های رشد مانند افزایش وزن، نرخ رشد ویژه، وزن نهایی و شاخص وضعیت نسبت به گروه شاهد به­ طور معنی­ داری بالاتر به ­دست آمد (0/05>p ). براساس نتایج به ­دست آمده از پارامترهای خون ­شناسی، تیمار تغذیه شده با 2/5 درصد عصاره زنیان دارای بیش ­ترین میزان هماتوکریت و هموگلوبین بوده و اختلاف معنی ‌داری با تیمار شاهد نشان داد (0/05>p ). در سایر پارامترهای خونی (گلبول‌ های سفید، گلبول ‌های قرمز، MCV، MCHC و MCH) اختلاف معنی‌ داری بین تیمارهای آزمایشی و گروه شاهد مشاهده نشد (0/05<p ). تغذیه ماهیان با 2/5 درصد عصاره زنیان فعالیت لیزوزیم و کمپلمان را به ­طور معنی ­داری بهبود بخشید (0/05>p ). در سایر پارامترهای بیوشیمیایی خون (ایمونوگلوبولین، آلبومین و پروتئین کل) اختلاف معنی­ داری بین سطوح مختلف عصاره زنیان و گروه شاهد دیده نشد (0/05<p ). بنابراین می‌ توان نتایج گرفت استفاده از عصاره گیاه زنیان در جیره غذایی ماهی قزل ­آلای رنگین­ کمان می‌ تواند منجر به بهبود فاکتورهای رشد و برخی از پارامترهای ایمنی گردد.

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