اثر غلظت های تحت کشنده دیازینون بر بیان ژن آروماتاز (cyp19a) در جنس ماده ماهی گورخری (Danio rerio)

نوع مقاله : ژنتیک

نویسندگان

گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

سم دیازینون یک سم پرکاربرد در کشاورزی است و می­ تواند به ­عنوان یک خطر برای تولیدمثل آبزیان به شمار می آید. در این تحقیق تأثیر دوزهای تحت­ کشنده سم دیازینون بر بیان ژن‌ آروماتاز (Cyp19a) درجنس ماده ماهی گورخری (Danio rerio) بررسی شد. بدین منظور 600 قطعه بچه ‌­ماهی ‌گورخری با میانگین وزنی 0/01±0/15 ‌گرم  در قالب 4 گروه آزمایشی (یک گروه شاهد و 3 تیمار با 3 تکرار) به ­مدت یک­ ماه در مواجه با غلظت­ های 0/8، 1/6 و 3/2 میلی­ گرم بر لیتر سم دیازینون قرار گرفتند. در انتهای دوره از بافت گناد نمونه ‌برداری و استخراج RNA انجام شد. برای سنتز cDNA از کیت Superscript RTase استفاده ‌شد و cDNA حاصله با استفاده از پرایمرهای ژن‌ آروماتاز و ژن بتا اکتین به‌عنوان ژن رفرنس در Real Time PCR مورد بررسی قرار گرفت. ارزیابی بیان ژن آروماتاز در تیمارهای 0/8، 1/6 و 3/2 میلی­ گرم بر لیتر سم دیازینون الگوی کاهشی وابسته به دوز را نشان داد و میزان بیان ژن آروماتاز به­ ترتیب 0/98، 0/90 و 0/70 برابر گروه شاهد بود، اما این کاهش معنی­ دار نبود. نتایج مطالعه حاضر نشان می ­دهد که سم دیازینون می ­تواند اثر منفی بر تکامل رسیدگی جنسی در جنس ماده ماهی زبرا داشته باشد. 

کلیدواژه‌ها


عنوان مقاله [English]

Effects of sublethal concentration of diazinon on the expression of aromatase (CYP 19a) gene in females Zebrafish (Danio rerio)

نویسندگان [English]

  • Masomeh Darvishi
  • Roghieh Safari
  • Ali Shabani
  • Seyed Hossein Hoseinifar
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Diazinon is a commonly used poison in agriculture and can be considered a risk for the reproduction of aquatic animals. This study investigated the effect of sub lethal concentration of diazinon on the expression of aromatase (CYP 19a) gene in zebrafish females (Danio rerio). For this study, 600 fish with average weight of 0.15±0.1 g divided in 4 groups with 3 replicates were exposed to concentrations of 0.8, 1.6 and 3.2 mg/ liter of diazinon for 1 month and compared to control group. At the end of trial, samplings were done from gonad tissue and RNA extracted. For cDNA synthesis, the Superscript RTase kit was used and Real Time PCR was done aromatase and beta-actin genes. Evaluation of aromatase gene expression in the diazinon-treated groups, 0.8, 1.6 and 3.2 mg/l, showed a dose dependent decrease and aromatase genes expression were 0.98, 0.90 and 0.70 respectively, but was not significant. The results showed that diazinon may have a negative effect on sex development in zebrafish females.

کلیدواژه‌ها [English]

  • Diazinon
  • aromatase
  • Gene expression
  • Zebrafish
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