Nucleotide specific variants of the Amelogenin gene allow sex verification in livestock

Document Type : (original research)

Authors

1 Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Department of Animal Sciences, Faculty of Agriculture, University of Tehran, Karaj, Iran

3 Department of Environmental Protection of East Azerbaijan Province, Tabriz, Iran

4 Iran Institute of Animal Science Research, Agricultural Research, Education and Extension Organization, Karaj, Iran

Abstract

 in the breeding system, often one livestock is more valuable than the other the purpose of the present study was to use specific nucleotide variants between the amelogenin genes for sex determination in wild and domestic herbivores (sheep, goat, cattle and deer). For this purpose, whole blood and dung were about 59 studied animals were prepared and genomic DNA was extracted by commercial kit according to its supplementary instructions. The primers studied were selected and synthesized based on conserved regions in the phylogenetic gene sequence in the Gene Bank. The polymerase chain reaction was optimized according to existing standard protocols. The results clearly showed the existence of a bond pattern difference between males and females. The amplified band size of these identical primers varied depending on the species and the breed within each species. In some species, the electrophoretic patterns were inevitably coupled to a false band, which fortunately did not interfere with the sex determination process (Usually depending on race and age of deer is 4 (male) to 12 (female)). According to the observations obtained in this study, differences in specific nucleotide variants between Amelogenin genes can be considered as a suitable tool for pre-selection and sex identification in animal species.

Keywords


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