اثر تنش حرارتی مصنوعی بر فراسنجه های کیفی اسپرم گاو پس از یخ گشایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 گروه مامایی، تولیدمثل و سلامت گله، دانشکده دامپزشکی، دانشگاه گنت، گنت، بلژیک

چکیده

در این آزمایش به ­منظور مطالعه اثر تنش حرارتی بر کیفیت اسپرم گاوهای نژاد آبی بلژیکی از سه رأس گاو نر آبی بلژیکی که در شرایط یکسان مدیریتی و تغذیه قرار داشتند برای اسپرم ­گیری استفاده شد. برای شبیه ­سازی اثرات تنش حرارتی بیضه دو راس گاو نر با استفاده از کیسه نایلونی عایق ­سازی شد. پس از دو هفته در مجموع تعداد پنج انزال از گاوهای نر جمع ­آوری شده و پس از ارزیابی ­های اولیه، منجمد شدند. پس از یخ­ گشایی، فراسنجه ­های جنبایی، ناهنجاری ­های اسپرم، یکپارچگی غشای آکروزومی، وضعیت کروماتین اسپرم ­ها، زنده ­مانی، تولید رادیکال ­های آزاد هیدروژن پراکسید و هیدروکسیل ارزیابی شدند. نتایج نشان داد که درصد جنبایی کل، جنبایی پیش­رونده و حرکت درمسیر مستقیم در گروه تحت تنش حرارتی به طور معنی­ داری پایین ­تر از گروه شاهد بود. هم­ چنین، درصد ناهنجاری ­های کل، اسپرم ­های با دم ­های گره خورده و اسپرم ­های با قطره پروتوپلاسمی سری در گروه تحت تنش حرارتی بیش ­تر از گروه شاهد بود (P≤0/05). براساس نتایج به ­دست آمده، تنش حرارتی باعث افزایش ناهنجاری ­های کروماتین اسپرم گاو شده (P≤0/05) اما اثر معنی ­داری بر افزایش آسیب ­های آکروزومی اسپرم ­ها نداشت. هم ­چنین، تنش حرارتی باعث کاهش زنده ­مانی اسپرم­ ها پس از یخ­ گشایی شده (P≤0/01) و میزان تولید پروکسید هیدروژن (H2O2) و رادیکال هیدروکسیل را افزایش می­ دهد (P≤0/05). براساس نتایج این آزمایش می‌ توان نتیجه گرفت که تنش حرارتی می ­تواند اثرات نامطلوبی بر ویژگی ­های کیفی اسپرم پس از انزال داشته و احتمالا باروری گاوها را نیز تحت تاثیر قرار دهد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of artificial heat stress on Belgian blue bull’s semen quality after thawing

نویسندگان [English]

  • Afshin Seifi Jamadi 1
  • Hamid Kohram 1
  • Mahdi Zhandi 1
  • Ann Van Soom 2
1 Department of Animal Science, Faculty of Agriculture and National Resources, University of Tehran, Karaj, Iran
2 Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Salisburylaan 133, 9820 Merelbeke, Belgium
چکیده [English]

 This study was aimed to evaluate whether high ambient temperature (heat stress, HS) can affect sperm quality parameters in Belgian blue bulls. For this purpose, semen samples were collected from three healthy mature Belgian blue bulls which were located in a breeding company under uniform feeding and management conditions. After semen collection, and the primary evaluations, the ejaculates with more than 60 percent total motility were subjected to the freezing process. After thawing the samples were used to assess the motion parameters, morphological abnormalities, acrosome integrity, chromatin condensation, viability, hydrogen peroxide (H2O2) and hydroxyl ion production. The results indicated that total and progressive motility and straight-line velocity were lower in the HS group compared to the control (P≤0.05), moreover, the generation of H2O2, hydroxyl ions, percentage of aberrant chromatin condensation, total morphological abnormal sperm cells, spermatozoa with bent tails and proximal protoplasmic droplets were higher in the HS compared to the control (P≤0.05) while the percentage pf damaged acrosome was not differ between HS and control (P≥0.05). In conclusion, these findings show that elevated ambient temperature can decrease the quality of frozen-thawed bull spermatozoa may leading to a lower fertility rate.

کلیدواژه‌ها [English]

  • Cryopreservation
  • Fertility
  • Free radicals
  • Temperature Humidity Index

مراجع

  1. Al-Kanaan, A.; König, S. and Brügemann, K., 2015. Effects of heat stress on semen characteristics of Holstein bulls estimated on a continuous phenotypic and genetic scale. Livestok Science. Vol. 177, pp: 15-24.
  2. Ashrafi, I.; Daghigh Kia, H.; Moghaddam, G. and Saberivand, A., 2017. Comparison of extracted low-density lipoproteins from egg yolk with lecithin, and commercially extender “Andromed” in bull's semen freezability. Animal Environment. Vol. 9, pp: 49-56.
  3. Bianchi, M.; Alves, R.; Furugen, A.; Andrade, C.; De, Paes, R.; Arruda, D.; Batissaco, L.; Florez-rodriguez, S.A.; Marcele, B.; Oliveira, M. De, Andrade, M.; Lançoni, R.; Mouro, G.; Romano, R.; Silva, V.; Diego, J.; Losano, D.A.; Rodrigues, C.; Nichi, M.; Carla, E. and Celeghini, C., 2016. Recovery of normal testicular temperature after scrotal heat stress in rams assessed by infrared thermography and its effects on seminal characteristics and testosterone blood serum concentration. Theriogenology. Vol. 86, pp: 795-805.
  4. Boni, R., 2019. Heat stress, a serious threat to reproductive function in animals and humans. Molecular Reproduction and Development. Vol. 2019, pp: 1-17.
  5. Brito, L.F.C.; Silva, A.E.D.F.; Barbosa, R.T.; Unanian, M.M. and Kastelic, J.P., 2003. Effects of scrotal insulation on sperm production, semen quality, and testicular echotexture in Bos indicus and Bos indicus x Bos taurus bulls. Animal Reproduction Science. Vol. 79, pp: 1-15.
  6. Castro, L.S.; De, Assis, P.M.; De, Siqueira, A.F.P.; Hamilton, T.R.S.; Mendes, C.M.; Losano, J.D.A.; Nichi, M.; Visintin, J.A. and Assumpção, M.E.O.A., 2016. Sperm Oxidative Stress Is Detrimental to Embryo Development : A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation. Oxidative Medicine and Cellular Longevity. Vol. 2016, pp: 1-12.
  7. Castro, L.S.; Siqueira, A.F.P.; Hamilton, T.R.S.; Mendes, C.M.; Visintin, J.A. and Assumpção, M., 2018. Effect of bovine sperm chromatin integrity evaluated using three different methods on in vitro fertility. Theriogenology. Vol. 107, pp: 142-148.
  8. Cheng, Y.; Liu, S.; Zhang, Ying, Su, D.; Wang, G.; Lv, C.; Zhang, Y.; Yu, H.; Hao, L. and Zhang, J., 2016. The effect of heat stress on bull sperm quality and related HSPs expression. Animal Biology. Vol. 66, pp: 321-333.
  9. De Behr, V.; Hornick, J.L.; Cabaraux, J.F.; Alvarez, A. and Istasse, L., 2001. Growth patterns of Belgian Blue replacement heifers and growing males in commercial farms. Livestok Production Science. Vol. 71, pp: 121-130.
  10. De, K.; Kumar, D.; Balaganur, K.; Saxena, V.K.; Thirumurugan, P.; Mohammed, S. and Naqvi, K., 2017. Effect of thermal exposure on physiological adaptability and seminal attributes of rams under semi-arid environment. Journal of Thermal Biology. Vol. 65, pp: 113-118.
  11. Dikmen, S. and Hansen, P.J., 2009. Is the temperature-humidity index the best indicator of heat stress in lactating dairy cows in a subtropical environment ? Journal of Dairy Science. Vol. 92, pp: 109-116.
  12. Donnell, L.O., 2014. Mechanisms of spermiogenesis and spermiation and how they are disturbed. Spermatogenesis. Vol. 4, No. 2, pp: 1-11.
  13. Durairajanayagam, D.; Agarwal, A. and Ong, C., 2015. Causes , effects and molecular mechanisms of testicular heat stress. Reproductive Biomedecine Online. Vol. 30, pp: 14-27.
  14. Fernandes, C.E.; Dode, M.A.N.; Pereira, D. and Silva, A.E.D.F., 2008. Effects of scrotal insulation in Nellore bulls (Bos taurus indicus) on seminal quality and its relationship with in vitro fertilizing ability. Theriogenology. Vol. 70, No. 9, pp: 1560-1568.
  15. Habeeb, A.A.; Gad, A.E. and Atta, M.A., 2018. Temperature-Humidity Indices as Indicators to Heat Stress of Climatic Conditions with Relation to Production and Reproduction of Farm Animals. International Journal of Biotechnology Recent Adv. Vol. 1, pp: 35-50.
  16. Hamerezaee, M.; Dehghan, S.F.; Golbabaei, F.; Fathi, A.; Barzegar, L. and Heidarnejad, N., 2018. Assessment of Semen Quality among Workers Exposed to Heat Stress : A Cross-Sectional Study in a Steel Industry. Safety at Health and Work. Vol. 9, pp: 232-235.
  17. Hamilton, T.R.D.S.; Mendes, C.M.; Castro, L.S. De, Assis, P.M. De, Siqueira, A.F.P.; Delgado, J.D.C.; Goissis, M.D.; Muiño-Blanco, T.; Cebrián-Pérez, J.Á.; Nichi, M.; Visintin, J.A. and Assumpção, M.E.O.D.Á., 2016. Evaluation of lasting effects of heat stress on sperm profile and oxidative status of ram semen and epididymal sperm. Oxidative Medicine and Cellular Longevity. Vol. 2016, pp: 1-12.
  18. Hansen, P.J., 2009. Effects of heat stress on mammalian reproduction. Philosophical Transactions of the Royal Society. Vol. 364, pp: 3341-3350.
  19. Hoflack, G.; Opsomer, G.; Rijsselaere, T.; Van Soom, A.; Maes, D.; De Kruif, A. and Duchateau, L., 2007. Comparison of Computer‐assisted Sperm Motility Analysis Parameters in Semen from Belgian Blue and Holstein Friesian Bulls. Reproduction in Domestic Animals. Vol. 42, pp: 153-161.
  20. Hoflack, G.; Opsomer, G.; Van Soom, A.; Maes, D.; de Kruif, A. and Duchateau, L., 2006. Comparison of sperm quality of Belgian Blue and Holstein Friesian bulls. Theriogenology. Vol. 66, pp: 1834-1846.
  21. Hussaini, S.; Zhandi, M.; Zare-Shahneh, A.; Sharafi, M.; Emamverdi, M. and Khodaeimotlagh, M., 2018. Folw cytometric evaluation of frozen-thawed bull semen in a semen extender containing ethanol. Animal Environment. Vol. 10, pp: 47-52.
  22. Ishii, T.; Matsuki, S.; Iuchi, Y.; Okada, F.; Toyosaki, S.; Tomita, Y.; Ikeda, Y. and Fujii, J., 2005. Accelerated impairment of spermatogenic cells in sod1-knockout mice under heat stress. Free Radical Research. Vol. 39, pp: 697-705.
  23. Lançoni, R.; Arruda, R.P.; De, Bianchi, M.; Alves, R. and Oliveira, L.Z., 2017. Validation of the CellRox Deep Red ® fluorescent probe to oxidative stress assessment in equine spermatozoa Validation of the CellRox Deep Red ® fluorescent probe to oxidative stress assessment in equine spermatozoa. Animal Reproduction. Vol. 14, No. 2, pp: 437-441.
  24. Leemans, B.; Gadella, B.M.; Sostaric, E.; Nelis, H.; Stout, T.A.E.; Hoogewijs, M. and Soom, A.V., 2014. Oviduct Binding and Elevated Environmental pH Induce Protein Tyrosine Phosphorylation in Stallion Spermatozoa. Biology of Reproduction. Vol. 91, No. 1, pp: 1-12.
  25. Llamas-Luceño, N.; Angrimani, D. de S.R.; de Cássia Bicudo, L.; Szymańska, K.J.; Van Poucke, M.; Demeyere, K.; Meyer, E.; Peelman, L.; Mullaart, E. and Broekhujise, M.L.W.J., 2020. Exposing dairy bulls to high temperature humidity index during spermatogenesis compromises subsequent embryo development in vitro. Theriogenology. Vol. 141, pp: 16-25.
  26. Lucio, A.C.; Alves, B.G.; Alves, K.A.; Martins, M.C.; Braga, L.S.; Miglio, L.; Alves, Bruna G, Silva, T.H.; Jacomini, J.O. and Beletti, M.E., 2016. Selected sperm traits are simultaneously altered after scrotal heat stress and play speci fi c roles in in vitro fertilization and embryonic development. Theriogenology. Vol. 86, No. 4, pp: 924-933.
  27. Mahmoud, A.M.A.; Gordts, S.; Vereecken, A.; Serneels, A.; Campo, R.; Rombauts, L. and Comhaire, F.H., 1998. Performance of the sperm quality analyser in predicting the outcome of assisted reproduction. International Journal Andrology. Vol. 21, pp: 41-46.
  28. Maíra Bianchi Rodrigues, A.; Furugen, A.; Andrade, C.D.; Arruda, R.P.D.; Batissaco, L. and Florez, S.A., 2015. An efficient technique to detect sperm reactive oxygen species. Biochemical Physiology. Vol. 4, No. 2, pp: 1-5.
  29. Malama, E.; Zeron, Y.; Janett, F.; Siuda, M.; Roth, Z. and Bollwein, H., 2017. Use of computer-assisted sperm analysis and fl ow cytometry to detect seasonal variations of bovine semen quality. Theriogenology. Vol. 87, pp: 79-90.
  30. Murphy, E.M.; Eivers, B.; O’Meara, C.M.; Lonergan, P. and Fair, S., 2018. Effect of increasing equilibration time of diluted bull semen up to 72 h prior to freezing on sperm quality parameters and calving rate following artificial insemination. Theriogenology. Vol. 108, pp: 217-222.
  31. Nasr‐Esfahani, M.H.; Razavi, S.; Mozdarani, H.; Mardani, M. and Azvagi, H., 2004. Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post‐ICSI. Andrologia Vol. 36, pp: 95-100.
  32. Nizański, W.; Partyka, A. and Rijsselaere, T., 2012. Use of Fluorescent Stainings and Flow Cytometry for Canine Semen Assessment. Reproduction in Domestic Animals. Vol. 47, pp: 215-221.
  33. Orgal, S.; Zeron, Y.; Elior, N.; Biran, D.; Friedman, E.; Druker, S. and Roth, Z., 2012. Season-Induced Changes in Bovine Sperm Motility Following a Freeze- Thaw Procedure. Journal of Reproduction and Development. Vol. 58, No. 2, pp: 212-218.
  34. Paul, C.; Murray, A.A.; Spears, N. and Saunders, P.T.K., 2008. A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice. Reproduction. Vol. 136, pp: 73-84.
  35. Paul, C.; Teng, S. and Saunders, P.T.K., 2009. A single, mild, transient scrotal heat stress causes hypoxia and oxidative stress in mouse testes, which induces germ cell death. Biology of Reproduction. Vol. 80, pp: 913-919.
  36. Pérez‐Crespo, M.; Pintado, B. and Gutiérrez‐Adán, A., 2008. Scrotal heat stress effects on sperm viability, sperm DNA integrity, and the offspring sex ratio in mice. Molecular Reproduction and Development. Vol. 75, pp: 40-47.
  37. Rahman, M.B.; Kamal, M.M.; Rijsselaere, T.; Vandaele, L.; Shamsuddin, M. and Van Soom, A., 2014a. Altered chromatin condensation of heat-stressed spermatozoa perturbs the dynamics of DNA methylation reprogramming in the paternal genome after in vitro fertilisation in cattle. Reproduction Fertility and Development. Vol. 26, pp: 1107-1116.
  38. Rahman, M.B.; Schellander, K.; Luceño, N.L. and Van Soom, A., 2018. Heat stress responses in spermatozoa: Mechanisms and consequences for cattle fertility. Theriogenology. Vol. 113, pp: 102-112.
  39. Rahman, M.B.; Vandaele, L.; Rijsselaere, T.; El-Deen, M.S.; Maes, D.; Shamsuddin, M. and Van Soom, A., 2014. Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway. Reproduction Fertility and Development. Vol. 26, pp: 245-257.
  40. Rahman, M.B.; Vandaele, L.; Rijsselaere, T.; Maes, D.; Hoogewijs, M.; Frijters, A.; Noordman, J.; Granados, A.; Dernelle, E.; Shamsuddin, M.; Parrish, J.J. and Van Soom, A., 2011. Scrotal insulation and its relationship to abnormal morphology, chromatin protamination and nuclear shape of spermatozoa in Holstein-Friesian and Belgian Blue bulls. Theriogenology. Vol. 76, pp: 1246-1257.
  41. Sabés-Alsina, M.; Johannisson, A.; Lundeheim, N.; Lopez-Bejar, M. and Morrell, J.M., 2017. Effects of season on bull sperm quality in thawed samples in northern Spain. Veterinary Records. Vol. 2017, pp:1-7.
  42. Sabés-Alsina, M.; Lundeheim, N.; Johannisson, A. and Morrell, J.M., 2019. Relationships between climate and sperm quality in dairy bull semen : A retrospective analysis. Journal of Dairy Science. Vol. 102, No. 6, pp: 5623-5633.
  43. Sabés-Alsina, M.; Tallo-Parra, O.; Mogas, M.T.; Morrell, J.M. and Lopez-Bejar, M., 2016. Heat stress has an effect on motility and metabolic activity of rabbit spermatozoa. Animal Reproduction Science. Vol. 173, pp: 18-23.
  44. Safaa, H.M.; Emarah, M.E. and Saleh, N.F.A., 2008. Seasonal effects on semen quality in black baladi and white New Zealand rabbit bucks. World Rabbit Science. Vol. 16, pp: 13-20.
  45. Shiraishi, K.; Matsuyama, H. and Takihara, H., 2012. Pathophysiology of varicocele in male infertility in the era of assisted reproductive technology. Urology. Vol. 19, pp: 538-550.
  46. Silanikove, N. and Koluman, N., 2015. Impact of climate change on the dairy industry in temperate zones : Predications on the overall negative impact and on the positive role of dairy goats in adaptation to earth warming. Small Ruminant Research. Vol. 123, pp: 27-34.
  47. Staub, C. and Johnson, L., 2018. Spermatogenesis in the bull. Animal. Vol. 12, pp: 27-35.
  48. Yaeram, J.A.; Setchell, B.P.A. and Maddocks, S.A., 2006. Effect of heat stress on the fertility of male mice in vivo and in vitro. Reproduction Fertility and Development. Vol. 18, pp: 647-653.
فصلنامه محیط زیست جانوری
دوره 12، شماره 4
دی 1399
صفحه 85-94

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