Evaluation of the effects of adding conventional urea and slow release urea to diets containing molasses on microbial fermentation by gas production method

Document Type : (original research)

Authors

1 Department of Animal Science Research, Safiabad Agricultural Research and Training Center and Natural Resources, Agricultural Research, Education and Extension Organization, Dezful, Iran

2 Department of Animal Sciences, Faculty of Animal Sciences and Food Industry, Khuzestan University of Agricultural Sciences and Natural Resources, Ahvaz, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

This experiment was conducted to investigate the effects of source and level of non-protein nitrogen, as well as the level of molasses added to high concentrate diets on gas production parameters. A completely randomized design with factorial (2×2×3) arrangement of 12 treatments with 5 replications was used. Factors were two types of non-protein nitrogen sources, common urea and slow-release urea (Nitroza) each in two levels (0.8 and 1.6%) and (0.9 and 1.8%) on dry matter basis of ration respectively, and molasses at three levels (0, 10 and 20%). With increasing of molasses, the amounts of cumulative gas production up to 24 hours of incubation, metabolisable energy, truly organic matter digestibility, organic matter digestibility and short chain fatty acids, increased (p < 0.05). Cumulative gas production was not affected by non-protein nitrogen source and its levels. Truly organic matter digestibility was higher in diets containing slow-release urea than conventional urea, but in other parameters there was no difference between the two non-protein nitrogen sources. In general, the results showed that the rate of ammonia release from conventional and slow release urea in the culture medium does not have a significant effect on in vitro fermentation, but adding 20% molasses on dry matter basis of ration, improved the gas production parameters.

Keywords

References

  1. Anele, U.Y.; Sudekum, K.H.; Hummel, J.; Arigbede, O.M.; Oni, A.O.; Olanite, J.A. and Jolaosho, A.O., 2011. Chemical characterization, in vitro dry matter and ruminal crude protein degradability and microbial protein synthesis of some cowpea (Vigna unguiculata) haulm varieties. Animal feed science and technology. Vol. 163, pp: 161-169.
  2. Blummel, M. and Ørskov, E.R., 1993. Comparison of gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Animal Feed Science and Technology. Vol. 40, pp: 109-119.
  3. Blummel, M.; Makkar, H.P.S. and Becker, K., 1997. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition. Vol. 77, pp: 24-34.
  4. Blummel, M. and Becker, K., 1997. The degradability characteristics of fifty-four roughages and roughage neutral detergent fibres as described by in vitro gas production and their relationship to voluntary feed intake. British Journal of Nutrition. Vol. 77, pp: 757-768.
  5. Ceconi, I.; Ruiz-Moreno, M.J.; DiLorenzo, N.; DiCostanzo, A. and Crawford, G.I., 2015. Effect of slow-release urea inclusion in diets containing modified corn distillers grains on total tract digestibility and ruminal fermentation in feedlot cattle. Journal of Animal Science. Vol. 93, pp: 4058-4069.
  6. Cherdthong, A. and Wanapat, M., 2010. Development of urea products as rumen slow-release feed for ruminant production: A review. Australian Journal of Basic and Applied Sciences. Vol. 4, pp: 2232-2241.
  7. Cone, J.W. and van Gelder, A.H., 1999. Influence of protein fermentation on gas production profiles. Animal Feed Science and Technology. Vol. 76, pp: 251-264.
  8. Cone, J.W.; Jongbloed, A.W.; Van Gelder, A.H. and De Lange, L., 2005. Estimation of protein fermentation in the large intestine of pigs using a gas production technique. Animal Feed Science and Technology. Vol. 123, pp: 463-472.
  9. DeFrain, J.M.; Hippen, A.R.; Kalscheur, K.F. and Schingoethe, D.J., 2006. Feeding Lactose to Increase Ruminal Butyrate and the Metabolic Status of Transition Dairy Cows1. Journal of Dairy Science. Vol. 89, pp: 267-276.
  10. Dryhurst, N. and Wood, C.D., 1998. The effect of nitrogen source and concentration on in vitro gas production using rumen micro organisms. Animal Feed Science & Technology. Vol. 71, pp: 131-143.
  11. Firkins, J.L.; Oldick, B.S.; Pantoja, J.; Reveneau, C.; Gilligan, L.E. and Carver, L., 2008. Efficacy of Liquid Feeds Varying in Concentration and Composition of Fat, Nonprotein Nitrogen, and Nonfiber Carbohydrates for Lactating Dairy Cows. Journal of Dairy Science. Vol. 91, pp: 1969-1984.
  12. Galina, M.A.; Guerrero, M.; Serrano, G.; Morales, R. and Haenlein, G.F.W., 2000. Effect of complex catalytic supplementation with non-protein nitrogen on the ruminal ecosystem of growing goats pasturing on shrub land in Mexico. Small Ruminant Research. Vol. 36, pp: 33-42.
  13. Galo, E.; Emanuele, S.M.; Sniffen, C.J.; White, J.H. and Knapp, J.R., 2003. Effects of a polymer-coated urea product on nitrogen metabolism in lactating Holstein dairy cattle. Journal of Dairy Science. Vol. 86, pp: 2154-2162.
  14. Getachew, G.; Makkar, H.P.S. and Becker, K., 2002. Tropical browses: contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. The Journal of Agricultural Science. Vol. 139, pp: 341-352.
  15. Highstreet, A.; Robinson, P.H.; Robison, J. and Garrett, J.G., 2010. Response of Holstein cows to replacing urea with with a slowly rumen released urea in a diet high in soluble crude protein. Livestock.
  16. Hristov, A.N. and Ropp, J.K., 2003. Effect of dietary carbohydrate composition and availability on utilization of ruminal ammonia nitrogen for milk protein synthesis in dairy cows. Journal of Dairy Science. Vol. 86, pp: 2416-2427.
  17. Menke, K.H. and Steingass, H., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal. Research. Development. Vol. 28, pp: 7-55.
  18. Menke, K.H.; Raab, L.; Salewski, A.; Steingass, H.; Fritz, D. and Schneider, W., 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. The Journal of Agricultural Science. Vol. 93, pp: 217-222.
  19. NRC. 2007. Nutrient requirements of small ruminants: sheep, goats, cervide, and new world camelids. National Academy Press, Washington, DC.
  20. Ørskov, E.R. and McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science. Vol. 92, pp: 499-503.
  21. Rooney, L.W. and Pflugfelder, R.L., 1986. Factors affecting starch digestibility with special emphasis on sorghum and corn. Journal of Animal Science. Vol. 63, pp: 1607-1623.
  22. Sannes, R.A.; Messman, M.A. and Vagnoni, D.B., 2002. Form of rumen-degradable carbohydrate and nitrogen on microbial protein synthesis and protein efficiency of dairy cows. Journal of Dairy Science. Vol. 85, pp: 900-908.
  23. Santos, A.S.; Ferreira, L.M.M.; Martin-Rosset, W.; Cone, J.W.; Bessa, R.J.B. and Rodrigues, M.A.M., 2013. Effect of nitrogen sources on in vitro fermentation profiles and microbial yield using equine caecal contents. Animal Feed Science and Technology. Vol. 182, pp: 93-99.
  24. SAS. 2003. SAS User’s Guide: Statistics, Version 9.1 Edition. SAS Institute, Cary, NC, USA.
  25. Sommart, K.; Parker, D.S.; Rowlinson, P. and Wanapat, M., 2000. Fermentation characteristics and microbial protein synthesis in an in vitro system using cassava, rice straw and dried ruzi grass as substrates. Asian Australian Journal of Animal Science. Vol. 13, pp: 1084-1093.
  26. Spanghero, M.; Nikulina, A. and Mason, F., 2018. Use of an in vitro gas production procedure to evaluate rumen slow release urea products. Animal Feed Science and Technology. Vol. 237, pp: 19-26.
  27. Taylor-Edwards, C.C.; Elam, N.A.; Kitts, S.E.; McLeod, K.R.; Axe, D.E.; Vanzant, E.S.; Kristensen, N.B. and Harmon, D.L., 2009. Influence of slow-release urea on nitrogen balance and portal-drained visceral nutrient flux in beef steers. Journal of Animal Science. Vol. 87, pp: 209-221.
  28. Theodorou, M.K.; Williams, B.A.; Dhanoa, M.S.; McAllan, A.B. and France, J., 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal feed science and technology. Vol. 48, pp: 185-197.
Journal of Animal Environment
Volume 12, Issue 4
December 2020
Pages 103-110

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