اثرات نانوذرات نقره بر خصوصیات کمی و کیفی اسپرم بلدرچین ژاپنی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، واحد کاشمر، دانشگاه آزاد اسلامی، کاشمر، ایران

10.22034/aej.2020.126250

چکیده

در تحقیق حاضر اثرات نانوذرات نقره بر خصوصیات کمی و کیفی اسپرم در بلدرچین ژاپنی بررسی گردید. اسپرم در سوسپانسیون حاوی غلظت‌های 0، 0/75، 0/125 و 0/250 قسمت در میلیون نانوذرات نقره رقیق شد. زنده‌ مانی با استفاده از رنگ‌ آمیزی ائوزین-نیگروزین تعیین شد. سلامت آکروزوم اسپرم بعد از 40 دقیقه تیمار با نانوذرات نقره در دمای 39 درجه سانتی ­گراد با محلول فرمالین- سیترات ارزیابی شدند. غظت اسپرم، جنبایی و جنبایی پیش ­رونده اسپرم اندازه­ گیری گردیدند. افزودن نانوذرات نقره به منی بلدرچین در سطوح 0/125 و 0/250 قسمت در میلیون درصد جنبایی و جنبایی پیش ­رونده اسپرم را به ­طور معنی­ داری کاهش داد. زنده­ مانی اسپرم در سطح  0/125 قسمت در میلیون کاهش یافت (0/01>p ). سلامت آکروزوم در تیمار 0/125 نانوذرات نقره کاهش یافت (0/05>p ). در تیمارهای حاوی 0/125 و 0/250 قسمت در میلیون، درصد اسپرم با آکروزوم سا لم (0/05>p ) در مقایسه با شاهد کاهش معنی‌ داری یافت (0/05>p ). ممکن است نانوذرات قادر به تخریب غشای آکروزومی باشند. نتایج این تحقیق نشان می دهد که نانوذرات نقره با تأثیر بر عملکرد اسپرم ممکن است اثرات سمی روی اسپرم داشته باشند و سبب مرگ اسپرم شوند.

کلیدواژه‌ها


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