اثرات نانوذرات نقره بر خصوصیات کمی و کیفی اسپرم بلدرچین ژاپنی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، واحد کاشمر، دانشگاه آزاد اسلامی، کاشمر، ایران

چکیده

در تحقیق حاضر اثرات نانوذرات نقره بر خصوصیات کمی و کیفی اسپرم در بلدرچین ژاپنی بررسی گردید. اسپرم در سوسپانسیون حاوی غلظت‌های 0، 0/75، 0/125 و 0/250 قسمت در میلیون نانوذرات نقره رقیق شد. زنده‌ مانی با استفاده از رنگ‌ آمیزی ائوزین-نیگروزین تعیین شد. سلامت آکروزوم اسپرم بعد از 40 دقیقه تیمار با نانوذرات نقره در دمای 39 درجه سانتی ­گراد با محلول فرمالین- سیترات ارزیابی شدند. غظت اسپرم، جنبایی و جنبایی پیش ­رونده اسپرم اندازه­ گیری گردیدند. افزودن نانوذرات نقره به منی بلدرچین در سطوح 0/125 و 0/250 قسمت در میلیون درصد جنبایی و جنبایی پیش ­رونده اسپرم را به ­طور معنی­ داری کاهش داد. زنده­ مانی اسپرم در سطح  0/125 قسمت در میلیون کاهش یافت (0/01>p ). سلامت آکروزوم در تیمار 0/125 نانوذرات نقره کاهش یافت (0/05>p ). در تیمارهای حاوی 0/125 و 0/250 قسمت در میلیون، درصد اسپرم با آکروزوم سا لم (0/05>p ) در مقایسه با شاهد کاهش معنی‌ داری یافت (0/05>p ). ممکن است نانوذرات قادر به تخریب غشای آکروزومی باشند. نتایج این تحقیق نشان می دهد که نانوذرات نقره با تأثیر بر عملکرد اسپرم ممکن است اثرات سمی روی اسپرم داشته باشند و سبب مرگ اسپرم شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of silver nanoparticles on quantity and quality characteristics of Japanese quail sperm

نویسندگان [English]

  • Saeid Taghipour
  • Reza Vakili
Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran
چکیده [English]

The present research, the effects of silver nanoparticles on the quantitative and qualitative characteristics of sperm in Japanese quail were investigated. The sperm was diluted in medium containing 0, 0.75, 0.125 and 0.250 ppm of silver nanoparticle. Sperm viability, Membrane integrity were assessed using the eosin-nigrosine test. Sperm acrosome health was evaluated after 40 minutes of treatment with silver nanoparticles at 39 ℃ by Formalin-citrate solution. Sperm concentration, motility, and progressive sperm motility were measured. Addition of silver nanoparticles to the semen of quail significantly decreased sperm motility at 0.25 and 0.250 ppm. Addition of silver nanoparticles in 0.125 and 0.250 ppm levels to quail semen significantly decreased motility and progressive motility(p < 0.05). Viability was significantly reduced in 0.125 and 0.250 ppm AgNPs. The acrosome integrity was significantly decreased at 0.125 silver nanoparticles (p < 0.05). The percentage of spermatozoa with an intact membrane (p < 0.05) were significantly decteased in treatments containg of 0.125 and 0.250 ppm silver nanoparticles in comparison to control group. The nanoparticles may be able to damage the acrosomal membrane. The results of this study suggest that silver nanoparticles may have toxic effects on sperm and may cause sperm death.

کلیدواژه‌ها [English]

  • Nanotechnology
  • Silver nanoparticles
  • Sperm
  • Japanese quail

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فصلنامه محیط زیست جانوری
دوره 12، شماره 4
دی 1399
صفحه 245-250

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