نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه میکروبیولوژی، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
2 گروه ژنتیک و بیوتکنولوژی، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
3 گروه زیست شناسی، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Various virulence factors are involved in the pathogenesis of Staphylococcus aureus. Proteins that mediate adhesion to the host cell surface are important factors in the binding and invasion of Staphylococcus aureus. The aim of this study was to evaluate the frequency of genes encoding adhesion proteins FnbA, FnbB, Cna, Efb, Ica, Bbp < /em> and SdrE in Staphylococcus aureus strains in Ahvaz. In this study, 231 Staphylococcus aureus specimens were isolated from 4 hospitals in Ahvaz after biochemical tests. DNA was extracted by phenol-chloroform method and the frequency of cna, fnbA, fnbB, efb, ica, bbp < /em> and sdrE genes was determined using specific primers for each gene by Multiplex PCR. In this study 16srRNA was used as internal control. Finally, the PCR products were applied on acrylamide gel. The frequency of genes studied in this study were as follows: fnbB gene (67.53%), fnbA gene (53.24%), ica gene (51.94%), cna gene (40.25%), efb (37.66%), sdrE (26.40%) and bbp < /em> gene (11.68%). Based on this study, the variable frequency of pathogenic genes in clinical isolates of Staphylococcus aureus confirms that bacterial virulence factors play an important role in bacterial invasion and pathogenicity. Given the importance of Staphylococcus aureus in creating a wide range of diseases and the role of various adhesins produced by this mass, complete information on the distribution of the aforementioned adhesins among isolated strains in Iran is essential.
کلیدواژهها [English]