Effect of microalga Chlorella vulgaris extract compared to vitamin C on collagen І and MMP-1 genes expression in human skin fibroblast cells

Document Type : (original research)

Authors

1 Department of Marine Biology, Faculty of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Iranian Fisheries Research Organization, Agricultural Research, Education and Extension Organization, Tehran, Iran

3 Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, Iran

Abstract

 Skin as one of the organ, plays an important role in many physical functions. In skin aging process, should be prevented collagen degradation and multiple enzymes increasing, including matrix metalloproteinase (MMp < sub>S). Chlorella vulgaris is a marine alga and its beneficial effects on skin make it a proper ingredient to be used in anti-aging products. In this study, the effect of C. vulgaris extract and vitamin C on type І collagen and MMP-1 production in the human cell line Hu02 was investigated. Chlorella was extracted using ultrasonication plus enzymatic hydrolysis, and Chlorella extract at different concentration was investigated for its effect on gene expression of collagen І and MMP-1 in Hu02 cells. First, it was investigated whether alga extract induced cytotoxicity in Hu02 cells. MTT assay showed that extract was non-toxic to Hu02 cells. Using quantitative PCR, it was confirmed that extract increased the gene expression of types І collagen and decreased the MMP-1 expression, comparing to the control group. Chlorella extract and vitamin C with a similar viability (97%) was used to investigate the effect of extract on mentioned genes expression, compared to vitamin C. Type І collagen increased in both alga and vitamin C groups. The gene expression of MMP-1 in both alga and vitamin C groups was significantly lower than the control group (p < 0.05). These findings indicate that C. vulgaris extract induces collagen synthesis and MMP-1 enzyme inhibition in Hu02 cells.

Keywords

References

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Journal of Animal Environment
Volume 12, Issue 4
December 2020
Pages 551-556

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