بررسی تنوع متاژنوم قارچی سیستم مداربسته و بیوفلاک پرورش ماهی کپورمعمولی (Cyprinus carpio) با استفاده از ITS2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

2 گروه زیست شناسی دریا، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

3 گروه میکروبی، پژوهشگاه بیوتکنولوژی کشاورزی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

4 گروه محیط‌ زیست، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

چکیده

استفاده از روش ­های فیلوژنتیک مولکولی جهت پیشرفت علم سیستماتیک و شناسایی تنوع قارچی بسیار پرکاربرد است. در میان مارکرهای فیلوژنتیک، ژن ITS به دلیل مناسب بودن برای بارکد کردن و داشتن اطلاعات زیاد به­ عنوان یکی از برجسته ­ترین روش­ های مطالعه جوامع قارچی معرفی شده است. هدف از این تحقیق ایجاد چارچوبی فیلوژنتیک به عنوان آغازی برای طبقه ­بندی قارچ ­ها و تجزیه و تحلیل جوامع آن ­ها در سیستم ­های آبزی پروری است. در این مطالعه بعد از یک دوره دو ماهه پرورش ماهی کپور معمولی (0/3 ± 21/5 گرم)، کیفیت آب و جامعه قارچی سیستم ­های پرورشی شامل بیوفلاک و مداربسته مورد ارزیابی قرار گرفتند. نتایج حاصل از پارامترهای کیفی آب اختلاف معنی دار نشان داد (0/05>p) هم چنین از لحاظ کیفیت آب هر دو سیستم شرایط مطلوبی را برای زیست ماهی فراهم نمودند. از لحاظ شناسایی جمعیت قارچی قسمت بزرگی از این جمعیت ­ها ناشناخته است و تنها گونه شناخته شده و غالب در هر دو سیستم مورد بررسی Homophron spadiceum است. بنابراین از لحاظ جوامع و گونه­ های شناخته شده تفاوتی مشاهده نگردید.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Fungal metagenome diversity of recirculating aquaculture and biofloc rearing systems of common carp (Cyprinus carpio) by using ITS 2

نویسندگان [English]

  • Mona Tabarrok 1
  • Jafar Seyfabadi 2
  • Gholamreza Salehi Jouzani 3
  • Habibollah Younesi 4
1 Department of Aquaculture, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
2 Department of Marine Biology, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
3 Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4 Department of Environmental Science, Faculty of Natural Resources, Tarbiat Modares University, Noor, Iran
چکیده [English]

Molecular methods have been used for systematic science and identification of fungal diversity. Among phylogenetic markers, the ITS gene has been identified as one of the most prominent methods of studying fungal communities. The purpose of this research is to classify fungi and analyze their communities in aquaculture systems. In this study, after two-month rearing period of common carp, water quality and fungal community of farming systems including biofloc and recirculating aquaculture system were evaluated. In this study, after two-month rearing period of common carp, water quality and fungal community of farming systems including biofloc and recirculating aquaculture system were evaluated. The results of this study indicated that the water quality parameters in both systems were well within the acceptable levels for common carp culture. The identification of fungal showed that a large part of this population is unknown. The only known and dominant species in both systems is Homophron spadiceum. Therefore, there was no difference in known species and communities.

کلیدواژه‌ها [English]

  • Biofloc
  • Recirculating aquaculture system
  • Fungi
  • ITS
  • Rearing system
  • Common carp
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