Evaluation of the effects of metformin and silybinin as polyphenols using the zebrafish (Danio rerio) diabetic model

Document Type : (original research)


1 Department of Aquaculture Science, Babol Branch, Islamic Azad University, Babol, Iran

2 Fatemeh Bootorabi, fatemeh.fi, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.

3 M. Reza Khorramizadeh, Professor and Director, Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, and Zebra fish core Facility (ZFIN ID : ZDB-LAB-190117-2), Endocrinology and Metabolism research Institute,



In this study, zebrafish was used as a biological model to induce type 2 diabetes mellitus through glucose.
Then, the effect of metformin and silibinin combination was examined on elevated blood glucose and intestinal tissues. Intestine tissue staining performed by hematoxylin and eosin protocol (H and E).
The combination of these two drugs had also a significant role in reducing glucose levels.
The body weight increased significantly in the control group which was affected by glucose concentration, with the lowest body weight gain observed in the metformin group.
Diabetic state causes weak absorption whereby the fish body demands to increase absorption level by enhancing the amount of acidic goblet cells thereby acidifying the environment in the gastric tracts.
Collectively, this study indicated that treatment with metformin and Silibinin could improve metabolic mediated performances by reducing the blood glucose, and ameliorating the intestinal inflammation in type 2 diabetic zebrafish model. .


Main Subjects