تاثیر افزایش مواد معدنی در جیره غذایی بر شاخص های رشد، فلور باکتریایی و مورفولوژی روده ماهی تیلاپیای نیل (Oreochromis niloticus)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 گروه شیلات، دانشکده مهندسی منابع طبیعی، دانشگاه صنعتی اصفهان، اصفهان، ایران

چکیده

هدف از این مطالعه، بررسی اثر افزایش مواد معدنی جیره بر شاخص های رشد و تغییر فلور باکتریایی روده و سلامتی ماهی تیلاپیای (Oreochromis sp) در یک سازگان مدار بسته بود. به این منظور 180 قطعه ماهی تیلاپیا  با متوسط وزن 3/8±30 گرم به 9 واحد آزمایشی معرفی گردید (3 واحد آزمایشی برای هر تیمار). تیمارهای آزمایش شامل سطوح مختلف 0، 25 و 75 درصد غلظت مواد مغذی در جیره غذایی با توجه به براورد نیاز گیاه در یک دوره پرورش در سیستم هیدروپونیک معمولی طراحی گردید. پس از طی 70 روز، از ساقه دمی 5 ماهی از هر تیمار خونگیری به عمل آمد و بافت روده جهت بررسی فلور باکتریایی و بافت شناسی نیز نمونه ­برداری گردید. نتایج به دست آمده حاکی از تغییر معنی­ دار (0/05>P) تعداد کل باکتری­ های روده تنها در تیمار حاوی 75 درصد مواد معدنی در مقایسه با سایر تیمارها بود (0/05>P). نتایج نشان داد که جیره­ های حاوی مواد معدنی  تاثیر معنی ­داری بر شاخص ­های بافت ­شناسی روده نظیر طول و قطر پرز، تعداد سلول­ های لوکوسیت و تعداد سلول­ های جامی دارد. به طور کلی، رشد و توسعه اپیتلیوم روده، احتمالاً با فزایش مواد معدنی در جیره تیلاپیا، شرایط زیستی بهتری را برای این ماهی ایجاد می کند.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of different levels of inorganic supplement in hydroponic system on hemato-immunological responses, intestine bacterial flora and morphology of Nile tilapia (Oreochromis niloticus)

نویسندگان [English]

  • Ebrahim Motaghi 1
  • Gholamreza Rafiee 1
  • Alireza Mirvaghefi 1
  • Eisa Ebrahimi 2
  • Bagher Majazi Amiri 1
1 Department of Fisheries, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Department of Fisheries, Faculty of Natural Resources Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده [English]

The aim of the present study is to investigate the effect of different levels of inorganic supplements in hydroponic system with the re-use of water on some parameters related to Nile tilapia health status. To address that, 180 fish weighing 30±3.8 g was divided into 9 experimental units (3 for each treatment). Three different treatments were applied, including 0, 25 and 75% of nutrient requirement for hydroponic system and these levels were added to the diet of fish. Following 70 days of dietary administration, blood samples were withdrawn for caudal vein of 5 fish. Intestine sample was also taken in order to determine the bacterial microflora and histological changes. Obtained results showed significant changed in hematological and immunological parameters in dietary treatment as compared with control (P<0.05). Total number of bacteria in intestine was significantly (P<0.05) elevated in treatment 3 as compared with other treatments. The applied diets had significant impacts on histological indices, including villi height and diameter, total number of leucocyte and mucus-secreting cells (P<0.05). Generally, epithelium development of intestine besides the improvement of hematological parameters and stimulation of immunity system could perhaps indicate the better health status of Nile tilapia following dietary manipulation by inorganic supplementation.

کلیدواژه‌ها [English]

  • Intestinal Microflora
  • Lysozyme activity
  • Hemoglobin
  • Mucus-Secreting cells
  • Aquaponic

مراجع

  1. رفیعی، غ.ر.، 1386. راهنمای آبکشت. انتشارات دانشگاه تهران. 160 صفحه.
  2. Ali, A., 2000. Probiotics in the fish farming. Evaluation of a bacterial mixture. PhD thesis Swedish University of Agriculture Sciences. Umea, Sweden.
  3. Bouizgarne, B., 2013. Bacteria for plant growth promotion and disease management. In Bacteria in Agrobiology: Disease Management; Maheshwari, D.K., Ed.; Springer: Berlin/Heidelberg, Germany. pp: 15-47.
  4. Burtis, C.A.; Ashwood, E.R. and Bruns, D.E., 2012. Tietz textbook of clinical chemistry and molecular diagnostics. 6th Elsevier Health Sciences Press, United States of America. 952 p.
  5. Courtois, L.A. and Meyerhoff, R.D., 1975. Effects of copper exposure on water balance. Bulletin of Environmental Contamination and Toxicology. Vol. 14, No. 2, pp: 221-224.
  6. Delaide, B.; Goddek, S.; Mankasingh, U.; Ragnarsdottir, K.V.; Jijakli, H. and Thorarinsdottir, R., 2015, Challenges of sustainable and commercial aquaponics. Sustain. Vol. 7, pp: 4199-4224.
  7. Dick, P. and Dixon, D., 1985. Changes in circulating blood cell levels of rainbow trout, Salmo gairdneri Richardson, following acute and chronic exposure to copper. Journal of Fish Biology. Vol. 26, No. 4, pp: 475-481.
  8. Francis, C.; Lieblein, G.; Gliessman, S.; Breland, T.A.; Creamer, N.; Harwood, R.; Salomonsson, L.; Helenius, J.; Rickerl, D.; Salvador, R.; Wiedenhoeft, M.; Simmons, S.; Allen, P.; Altieri, M.; Flora, C. and Poincelot, R., Agroecology: The Ecology of Food Systems. J. Sustain. Agric. Vol. 22, pp: 99-118.
  9. Gildberg, A.; Mikkelsen, H.; Sandaker, E. and Ringo, E., 1997. Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of Atlatic cod (Gadus morhua). Hydrobiologia. Vol. 352, pp: 279-285.
  10. Heath, A.G., 1995. Water pollution and fish physiology. CRC press, Washington. 384 p.
  11. Hood, S.K. and Zottola, A., 1988. Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells. Journal of Food Science. Vol. 53, pp: 1514-1516.
  12. Hu, Z.; Lee, J.W.; Chandran, K.; Kim, S.; Brotto, A.C. and Khanal, S.K., 2015. Effect of plant species on nitrogen recovery in aquaponics. Bioresource Technol. Vol. 188, pp: 92-98.
  13. Karim, M.; Jeong, S.Y.; Yoon, S.J.; Cho, S.J.; Kim, Y.H.; Kim, M.J.; Ryu, E.Y. and Lee, S.J., 2008. Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios. Journal of Bioscience and Bioengineering. Vol. 106, pp: 498-502.
  14. Kori-Siakpere, O. and Ubogu, E.O., 2008. Sublethal haematological effects of zinc on the freshwater fish, Heteroclarias(Osteichthyes: Clariidae). African Journal of Biotechnology. Vol. 7, No. 12, pp: 2068-2073.
  15. Lennard, W.A. and Leonard, B.V., 2006. A comparison of three different hydroponic subsystems (gravel bed, floating and nutrient film technique) in an Aquaponic test Aquac. Int. Vol. 14, No. 6, pp: 539-550.
  16. Lie, O.; Evansen, O.; Sorensen, A. and Froysadal, E., 1989. Study of lysozyme in some fish species. Dis. Aquat.Org. Vol. 6, pp: 1-5.
  17. Love, D.C.; Fry, J.P.; Genello, L.; Hill, E.S.; Frederick, J.A.; Li, X. and Semmens, K., 2014. An international survey of aquaponics practitioners. PLoS Vol. 9, No. 7, e102662.
  18. McKim, J.; Christensen, G. and Hunt, E.P., 1970. Changes in the blood of brook trout (Salvelinus fontinalis) after short term and long-term exposure to copper. Journal of the Fisheries Board of Canada. Vol. 27, No. 10, pp: 1883-1889.
  19. Moriarty, D.J.W., 1998. Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture. Vol. 164, pp: 351-358.
  20. Moyner, K.; Roed, K.H.; Sevatdal, S. and Heum, M., 1993. Changes hn non-specific immune parameters in Atlantic Salmon, Salmo salar L, induced by Aeromonas salmonicida infaction. Fish. Sellfish Immunol. Vol. 3, pp: 253-265
  21. Noga, E., 1995. Fish disease: Diagnosis and treatment2nd ed,. Watsworth Publishing, Co., USA. 519 p.
  22. Ololade, I. and Ogini, O., 2009. Behavioural and hematological effects of zinc on African catfish, Clarias gariepinus. International Journal of Fisheries and Aquaculture. Vol. 1, No. 2, pp: 22-27.
  23. Pulvenis, J.F., 2016. Fisheries and Aquaculture topics. The State of World Fisheries and Aquaculture (SOFIA). Topics Fact Sheets. Fisheries and Aquaculture Department.; Rome,
  24. Rafiee, Gh.R. and Saad, Ch.R., 2006. The effect of natural zeolite (Clinoptilolite) on Aquaponics production of red tilapia (Oreochromis ) and Lettuce (Lactuca sativa var. longifolia), and improvement of water quality. Iranian Journal of Agricultural Sciences and Technology. Vol. 8, No. 4, pp: 313-322.
  25. Rafiee, Gh.R. and Saad, Ch.R., 2005. Nutrient cycle and sludge production during different stages of red tilapia (Oreochromis ) growth in a recirculating aquaculture system. Aquaculture. Vol. 244, No. 1-4, pp: 109-118.
  26. Rakocy, J.E.; Losordo, T.M. and Masser, M.P., 1992. Recirculating Aquaculture Tank Production Systems: Integrating Fish and Plant Culture. SRAC Publication, Southern region aquaculture center, Pub. No: 454, USA. 12.
  27. Rakocy, J.E., 2012. Aquaponics-Integrating Fish and Plant Culture. In; Wiley-Blackwell. pp: 344-386.
  28. Resh, H.M., Hydroponic food production: a definitive guidebook for the advanced home gardener and the commercial hydroponic grower; Boca Raton, FL: CRC Press: Boca Raton, FL,
  29. Ruiz, G., Jeison, D. and Chamy, R., 2003. Nitrification with high nitrite accumulation forthe treatment of wastewater with high ammonia concentration. Water Research, 37: 1371–1377.
  30. Sampath, K.; James, R. and Ali, KA. (1998). , Effects of copper and zinc on blood parameters and prediction of their recovery in Oreochromis mossambicus (Pisces: Cichlidae). Indian Journal of Fisheries, 45 (2): 129-139.
  31. Singh, D.; Nath, K.; Trivedi, S. and Sharma, Y., 2008. Impact of copper on haematological profile of freshwater fish, Channa punctatus. Journal of Environmental Biology. Vol. 29, No. 2, pp: 253.
  32. Soivio, A. and Nikinmaa, M., 1981. Swelling of erythrocytes in relation to the oxygen affinity of the blood of the rainbow trout, Salmo gairdneri In: Pickering, AD. (ed). Stress and fish. Academic Press. pp: 103-119.
  33. Sonneveld, C. and Voogt, W., Plant Nutrition of Greenhouse Crops; Springer Netherlands.
  34. Svobodova, Z.; Vykusova, B.; Machova, J.; Müller, R. and Lloyd, R., 1994. Sublethal chronic effects of pollutants on freshwater fish. In: Muller R. and Lloyd R. Lugano. pp: 39-52.
  35. Steffen, W.; Richardson, K.; Rockström, J.; Cornell, S.E.; Fetzer, I.; Bennett, E.M.; Biggs, R.; Carpenter, S.R.; De Vries, W.; De Wit, C.A.; Folke, C.; Gerten, D.; Heinke, J.; Mace, G.M.; Persson, L.M.; Ramanathan, V.; Reyers, B. and Sörlin, S., 2015, Planetary boundaries: Guiding human development on a changing planet. Science. Vol. 80, pp: 347.
  36. Timmons, M.B. and Ebeling, J.M., Recirculating Aquaculture; 3rd ed.; Ithaca Publishing Company LLC: Ithaca, NY.
  37. Tukmechi, A.; Morshedi, A. and Delirezh, N., 2007. Changes in Intestinal Microflora and Humoral Immune Response Following Probiotic Administration in Rainbow Trout (Oncorhynchus mykiss). Journal of Animal and Veterinary Advancess. Vol. 6, No. 10, pp: 1183-1189.
  38. Wongkiew, S.; Hu, Z.; Chandran, K.; Lee, J.W. and Khana, S.K., 2017. Nitrogen transformations in aquaponic systems: A review. Aquacultural Engineering. Vol. 76, pp: 9-19.
  39. Yildiz, H.Y.; Robaina, L.; Pirhonen, J.; Mente, E.; Domnguez, D. and Parisi, G., 2017. Fish welfare in aquaponic systems: Its relation to water quality with an emphasis on feed and faeces-A review. Water (Switzerland). Vol. 9, pp: 1-17.

 

فصلنامه محیط زیست جانوری
دوره 13، شماره 2
مرداد 1400
صفحه 267-276

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