مقایسه استفاده از لاکتوفرین به صورت مخلوط با غذا و اسپری بر پاسخ های فیزیولوژیک ماهی شانک زرد باله (Acanthopagrus latus)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات و بیولوژی دریا، پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران

2 گروه شیلات، دانشکده علوم و فناوری نانو و زیستی، دانشگاه خلیج فارس، بوشهر، ایران

3 گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

هدف از این مطالعه، مقایسه­ استفاده از لاکتوفرین به صورت مخلوط با غذا و اسپری بر روی فاکتورهای خونی و پاسخ ایمنی غیر اختصاصی شانک زرد باله (Acanthopagrus latus) با میانگین وزنی 0/3±10 گرم بود. این مطالعه در قالب یک طرح کاملاً تصادفی با 3 تیمار و 3 تکرار در داخل مخازن فایبرگلاس 300 لیتری انجام شد. ماهیان به ­مدت 35 روز با جیره ­های حاوی 0 و 800 میلی­ گرم لاکتوفرین در هر کیلوگرم غذا به صورت اسپری و مخلوط تغذیه شدند. در پایان آزمایش نمونه­ های خون و پلاسما جمع ­آوری شد. نتایج حاصل نشان داد که لاکتوفرین جیره پاسخ ایمنی غیراختصاصی شانک زرد باله را تغییر نداد به جز فعالیت لیزوزیم (0/05<P). ظرفیت باند شدن آهن و میزان آهن پلاسما به ­صورت معنی­ داری در ماهیان تغذیه شده با 800 میلی­ گرم لاکتوفرین به ­صورت مخلوط معنی­ داری تفاوت داشت (0/05>P). میزان هموگلوبین و تعداد گلبول­ های قرمز و سفید در گروه شاهد با تیمار 800 میلی­ گرم لاکتوفرین به ­صورت مخلوط اختلاف معنی ­دار نشان دادند (0/05>P). با این حال میزان هماتوکریت به ­وسیله لاکتوفرین جیره به صورت اسپری تحت تاثیر قرار گرفت (0/05>P). به ­طور کلی، این مطالعه نشان داد که فاکتورهای خونی و بیوشیمیایی به ­وسیله لاکتوفرین جیره تحت تاثیر قرار گرفت. با این حال، می توان نتیجه­ گیری کرد که تغذیه ماهی شانک زردباله با جیره حاوی 800 میلی­ گرم لاکتوفرین به صورت اسپری برای یک دوره 5 هفته ­ای پاسخ ایمنی غیراختصاصی را افزایش می ­دهد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Comparison of the use of lactoferrin in combination with feed and sprays in physiological responses of yellowfin seabream (Acanthopagrus latus)

نویسندگان [English]

  • Vahid Morshedi 1
  • Afsaneh Eesmaili 2
  • Shirin Hamedi 1
  • Ebrahim Sotoudeh 2
  • Gholamreza Badzohreh 3
  • Asma Ahmadi 2
  • Rezvan Tamadoni 2
1 Department of Fisheries, Persian Gulf Research Institute, Persian Gulf University, Bushehr, Iran
2 Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Iran
3 Department of Fisheries, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran
چکیده [English]

The aim of this study was to compare the effects of dietary lactoferrin (LF) in combination with feed and sprays on hematological factors and non-specific immune response of yellowfin seabream (Acanthopagrus latus) with an average weight of 10±0.3 g. This study was carried out in a completely randomized design with three treatments and replications in fiberglass tanks with 300 liters volume. Fish were fed with feed containing 0 and 800 mg lactoferrin spray and mix per kg feed for a period of 35 days. At the end of the experiment, blood and plasma samples were collected. The obtained results indicated that dietary LF did not change yellowfin seabream non-specific immune response except for lysozyme activity (P>0.05). TIBC and iron plasma significantly vary in fish fed on 800 mg spray and mixed lactoferrin respectively (P<0.05). Hemoglobin value and red and white blood cells count showed significant differences between the control groups with 800 mg mixed lactoferrin (P<0.05). Although hematocrit value was influenced by spray lactoferrin (P<0.05). Overall, this study showed that hematological and biochemical factors were affected by dietary lactoferrin. Nonetheless, it can be concluded that feeding of yellowfin seabream on the diet supplemented with 800 mg spray lactoferrin for a period of 5 weeks enhance the non-specific immune response.
 

کلیدواژه‌ها [English]

  • Lactoferrin
  • Innate immunity
  • Blood biochemical factors
  • Hematological factors
  • Yellowfin seabream (Acanthopagrus latus)

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فصلنامه محیط زیست جانوری
دوره 13، شماره 3
مهر 1400
صفحه 239-246

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