Evaluation of Bacillus and Lactobacillus strains isolated from Epinephelus coioides regarding enzyme activities

Document Type : Disease

Authors

1 Department of Fisheries, Natural Resources Faculty, Behbahan Khatam alanbia University of Technology, Behbahan, Khozestan, 63616-47189, Iran.

2 Department of Microbiology, Faculty of biology, Islamic Azad University Falavarjan Branch, 63618-16957, Iran.

3 Department of Microbiology, Faculty of biology, Islamic Azad University Falavarjan Branch, 8179943373, Iran

Abstract

This study be done with purpose of comparison Bacillus and Lactobacillus strains isolated from the digestive tract of Epinephelus coioides regarding amount of enzyme activities. Thus aseptically, from different parts of the digestive system of E.coioides were sampled. After culturing in specific media and preforming biochemical tests and purification of different bacterial strains, specific activity of amylase, protease and lipase enzymes were measured by use of specific substrates in six strains of Bacillus genus and two strains of  Lactobacillus genus. The findings indicate that specific activity of each of three enzymes in the strains of  Bacillus genus is more than the strains of  Lactobacillus genus. Regarding activity of protease, B9 strain had maximum activity with average 6.50 IU/mg protein which had significant difference (in level 0.05) with other strains. Respectively, maximum and minimum amylase activity observed inB9 strain of Bacillus (3/30 IU/mg protein) and L4 strain of Lactobacillus (0.43 IU/mg protein) which they had significant difference (in level 0.05), together. Also, maximum lipase activity observed in B9 (1.80 IU/mg protein) and B3 (1.66 IU/mg protein) strains of  Bacillus which had significant difference (in level 0.05) with other strains. 

Keywords

Journal of Animal Environment
Volume 7, Issue 4 - Serial Number 4
April 2016
Pages 179-186

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