The effect of bivalent vaccine, streptococcus / yersinios on some of the blood and Immunologic factors in rainbow trout, Oncorhynchus mykiss

Document Type : Disease

Authors

1 Shahid Motahary Cold Water Fishes Genetic and breeding Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Yasoj, Iran

2 Department of Clinical sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

10.22034/aej.2021.268851.2451

Abstract

Streptococcosis and Yersiniosis are important diseases for the rainbow trout (Oncorhynchus mykiss) in our country. The aim of this study was to compare the efficacy of divalent vaccine (Streptococcus iniae and Yersinia rockeri) in the immune responses contains, serum lysozyme rate, complement activation, bactericidal, respiratory burst (NBT), total protein, albumin, globulin and some hematological indices contains, RBC and WBC count, hematocrit and haemoglobin in rainbow trout. In this study three hundred rainbow trout fingerlings (25±2g) were randomly divided into four equal groups in triplicates. Treatments contains Streptococcosis vaccine, Yersiniosis vaccine, bivalent vaccine and control group. Fish in vaccinated treatment intraperitonealy injected with 100 microliter vaccine on the first day. The control group was injected with sterile PBS, identical to vaccinated group. Blood samples were collected from vaccinated and non-vaccinated groups at days 0, 30, and 60 of study. The results demonstrated that lysozyme rate in bivalent vaccine were significantly higher at day 60 post-immunization compared to the control group (p<0.05). A significant increase was observed in the complement activation in bivalent vaccine at day 30 and 60 post-vaccination compared to the other groups(p<0.05). Also this results demonstrated that number of WBC in vaccinated groups were higher than control treatment. Generally, it can be concluded that the administration of bivalent Streptococosis/Yersiniosis vaccine provided appropriate protection against disease and effectively induced the immune response against these two diseases which is comparable to either of the vaccines separately. Therefore, investigation of the efficiency of the bivalent vaccine at commercial level is highly warranted.

Keywords

Main Subjects

References

  1. Rastiannasab, A., Mousavi, S., Zolgharnein, H. and Hosseinzadeh Sahafi, H., 2017. Effect of the food supplemented probioenzyme on expression of immune related genes and redmouth disease (yersiniosis) control in rainbow trout (Oncorhynchus mykiss). Iranian Scientific Fisheries Journal. 26(1): 153-166. (In Persian)
  2. Annual Statistics of Iranian Fisheries. 2021. Planning and development office of Iranian Fisheries Organization. Irran. Tehran. (In Persian)
  3. The state of world fisheries and aquaculture. 2020. http://www.fao.org/publications/sofia/ 2020/en/.
  4. Faeed, M. and Ramezani, B., 2018. Bacterial Study of Streptococcosis in Infected fish and Antibiotic Resistance in Rainbow trout (Oncorhynchus mykiss) farms in Guilan Province. Journal of Aquaculture Development. 12(2):
    103-112. (In Persian)
  5. Soltani, M., Mousavi, Sh., Ebrahimzadeh Mousavi, H., Mirzargar, S., Taheri Mirghaed, A., Shafiei, Sh., Shohreh, P. and Mohammadian, S., Molecular study of Yersinia ruckeri distribution, the causative agent of yersiniosis in some farmed rainbow trout of Iran. Iranian Veterinary Journal. 10(1): 59-67. (In Persian)
  6. Pridgeon, J.W. and Klesius, P.H., 2011. Development and efficacy of a novobiocin resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus). Vaccine. 29(35): 5986-5993.
  7. Evensen, Ø., 2016. Development of fish vaccines: Focusing on methods. In Fish Vaccines; Adams, A., Ed.; Springer: Basel, Switzerland. 53-74.
  8. Ma, J., Bruce, T.J., Jones, E.M. and Cain, K.D., 2019. A review of fish vaccine development strategies: conventional methods and modern biotechnological approaches. Microorganisms. 7(11): 569-587.
  9. Adams, A., 2019. Progress, challenges and opportunities in fish vaccine development. Fish & shellfish immunology. 90: 210-214.
  10. Azhdahakoshpour, A., Payghan, R., Ahangarzadeh, M. and Mohseninejad, L., 2018. Incidence of Vibriosis disease of Cultured Asian seabass (Lates calcarifer) in cage and pond farms. Journal of Marine Fishes. 2(3): 27-33. (In Persian)
  11. Karami, E., Alishahi, M., Tabandeh, M., Ghorbanpour, M. and Mohammadian, T., 2017. Evaluation of immunogenicity of bivalent vaccine Streptococosis / Lactococcosis in rainbow trout (Oncorhynchus mykiss). Journal of Animal Environment. 9(4): 199-206. (In Persian)
  12. Shomaker, C.A. and Klesius, P., 2012. Bivalent vaccination of sex reversed hybrid tilapia against Streptococcus iniae and Vibrio vulnificus. 354-355: 45-49.
  13. Bastardo, A., Ravelo, C., Castro, N., Calheiros, J. and Romalde, J.L., 2012. Effectiveness of bivalent vaccines against Aeromonas hydrophila and Lactococcus garvieae infections in rainbow trout Oncorhynchus mykiss (Walbaum). Fish & Shellfish Immunology. 32(5): 756-761.
  14. Brata, O., 1993. Veterinary Clinical Immunology Laboratory 2. Bar-Lab Inc. 24-25.
  15. Nayak, S.K., Swain, P., Nanda, P.K., Dash, S., Shukla, S., Meher, P.K. and Maiti, N.K., 2010. Effect of endotoxin on the immunity of Indian major carp, Labeo rohita. Fish & shellfish immunology. 24(4): 394-399.
  16. Swain, P., Sahoo, P.K. and Ayyappan, S., (Eds) 2007. Fish & shellfish immunology, Navendra Publishing House, D., Woo, P.T.K. and Bruno, D.W., (eds.), 1999. Fish Diseases and Disorders, Volume 3: Viral, Bacterial and Fungal Infections, CABI, U.K., 874 p. Delhi. 239: 1-12.
  17. Kajita, Y., Sakai, M., Atsuta, S. and Kobayash, M., 1990. The immunonodulatory effects of levamisole on rainbow trout (Oncorhynchus mykiss). Fish Pathology. 25(2): 93-98.
  18. Feldman, B.F., Zinkl, J.G. and Jain, N.C., 2000. Schalm's Veterinary Hematology. 5th ed. Lippincott Williams & Wilkins. 1124: 67-73.
  19. Thrall, M.A., Baker, D.C. and Lassen, E.D., 2004. Veterinary Hematology and Clinical Chemistry: Text and Clinical Case Presentations Set. John Wiley & Sons. UK, London. 241-402.
  20. Faghani, T., Azari Takami, Gh., Kousha, A. and Faghani, S., 2008. Surveying on alginic acid and anti streptococcus vaccine effects on the growth performance, survival rate, hematological parameters in rainbow trout (Oncorhynchos mykiss). World Journal of Zoology. 3(2): 54-58.
  21. Alishahi, M., Ranjbar, M.M., Ghorbanpour, M., Peyghan, R., Mesbah, M. and jalali, M., 2010. Effects of dietary Aloe vera on some specific and nonspecific immunity in the common carp (Cyprinus carpio). International Journal of Veterinary Research. 4(3): 189-195.
  22. Alishahi, M., Esmaeili Rad, A., Zarei, M. and Ghorbanpour, M., 2014. Effect of dietary chitosan on immune response and disease resistance of Cyprinus carpio. Iranian Journal of Veterinary Medicine. 8(2): 125-133.
  23. Karami, E., Alishahi, M., Ghorbanpour, M., Tanande, M. and Mohammadiyan, T., 2019. Serum antibody response of rainbow trout, Oncorhynchus mykiss to two species of pathogenic bacteria; Streptococcus iniae and Lactococcus garviae. Iranian Scientific Fisheries Journal. 28(1):1-8. (In Persian)
  24. Alishahi, M., Saidimanesh, M., Mesbah, M. and Zarei, M., 2016. Effect of nanochitosan on immunogenicity of Aeromonas hydrophila oral vaccine in Cyprinus carpio. Iranian Veterinary Journal. 12(1): 53-65. (In Persian)
  25. Choi, S.H., Park, K.H., Yoon, T.J., Kim, J.B., Jang, Y.S. and Choe, C.H., 2008. Dietary Koren mistletoe enhances cellular non- specific immune responses and survival of Japanese eel (Anguilla japonica). Fish Shellfish Immunol. 24 (1): 67-73.
  26. Ellis, A.E., 1990. Immunity to bacteria in fish. Fish & Shellfish Immunology. 9: 291-308. For Responsible fisheries.NO.5/suppl.4.Rome. 53p.
  27. Wang, E., Wang, J., Long, B., Kaiyu, W., Yang, H., Qian Huang, H., Ouyang, P. and Lai, H., 2016. Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae. Sci. Rep. 4(6): 29310 p.
  28. Soltani, M., Alishahi, M., Mirzargar, S. and Nikbakht, G., 2007. Vaccination of rainbow trout against Streptococcus iniae infection: comparison of different routes of administration and different vaccines. Iranian Journal of Fisheries Sciences. 7(1): 129-140.
  29. Zhou, X., Xing, J., Tang, X. and Zhan, W., 2018. Evaluation of bivalent vaccines candidates among VAA, OmpK and OmpR from Vibrio anguillarum in flounder (Paralichthys olivaceus). Dev Comp Immunol. 85: 1-9.
  30. Xing, J., Wang, L., Zhen, M., Tang, X. and Zhan, W., 2018. Variations of T and B lymphocytes of flounder (Paralichthys olivaceus) after Hirame novirhabdovirus infection and immunization. Mol Immunol. 96: 19-27.
Journal of Animal Environment
Volume 15, Issue 1
June 2023
Pages 213-220

Files

Share

How to cite

Statistics