The study of different somatic cell count levels on lipolysis process of white brine cheese

Document Type : Nutrition

Authors

1 Department of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Food Science and Industry, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

10.22034/aej.2021.265440.2440

Abstract

Somatic cell contains plasmin enzyme which hydrolyses milk protein; Therefore, a large amount of milk is removed during cheese making and the cheese production efficiency is reduced. So, the aim of this study was to estimate the changes of ripening with the attention to lipolysis process by monitoring the releasing of free fatty acids during ripening period. Three levels of somatic cell count in milk were classified as follows: Treatment 1: low somatic cell count in milk (0.7×105) cell/ml as control sample. Treatment 2: medium somatic cell count in milk (4.5×105) cell/ml Treatment 3: high somatic cell count in milk (1.2×106) cell/ml. The profile of free fatty acids was obtained by GC method. All free fatty acid including Short chain free fatty acid (SCFFA) and Long chain free fatty acids (LCFFA) increased during 65 days of ripening period. Oleic and palmitic fatty acids were predominant on the fifth day of the ripening period. However, treatment 3 on days 35 and 65 of the ripening period of white brine cheeses had the highest amount of oleic and palmitic acids. According to the results, treatment 1 was introduced as the best treatment

Keywords


  1. Khaleghkhah, E., Ezzatpanah, H., Mashhadi Akbar Boujar, M., Guiviyanrad, M.H., Seif Hashemi, S. and Motamed, R., 2013. The effect of different somatic cell levels saturated free fatty acids of raw milk. J. Sci. Res. Animal Sci. 12: 63-79.
  2. Halasa, T., Nielen, M., Huirne, R.B.M. and Hogeveen, H., 2009. Stochastic bio-economic model of bovine intramammary infection. J. Livestock Sci. 124: 295-305.
  3. Viguier, C., Arora, S., Gilmartin, N., Welbeck, K. and O’Kennedy, R., 2009. Mastitis detection: current trends and future perspectives. J. Trends Biotechnol. 27: 486-493.
  4. Barlowska, J., Litwinczuk, Z., Wolanciuk, A. and Brodziak, A., 2009. Relationship of somatic cell count to daily yield and technological usefulness of milk from different breeds of cows. Polish J. Vet. Sci. 12: 75-79.
  5. Hagnestam, C., Emanuelson, U. and Berglund, B., 2007. Yield losses associated with clinical mastitis occurring in different weeks of lactation. J. Dairy Sci. 90: 2260-2270.
  6. Grohn, Y.T., Wilson, D.J., Gonzalez, R.N., Hertl, J.A., Schulte, H., Bennett, G. and Schukken, Y.H., 2004. Effect of pathogen-specific clinical mastitis on milk yield in dairy cows. J. Dairy Sci. 87: 3358-3374.
  7. Raynal-Ljutovac, K., Pirisi, A., De Cremoux, R. and Gonzalo, C., 2007. Somatic cells of goat and sheep milk: analytical, sanitary, productive and technological aspects. J. Small Ruminant Res. 68: 126-144.
  8. Farajzadeh, J., Shahab Lavasani, A. and Eshaghi, M., 2020. The production of low fat confectionary cream by using Milk Protein Concentration. Journal of Animal Environment. 12(1): 435-442. (In Persian)
  9. Azzara, C.D. and Dimick, P.S., 1985. Lipoprotein lipase activity of milk from cows with prolonged subclinical mastitis. J. Dairy Sci. 68: 3171-3175.
  10. Fitz-Gerald, C.H., Deeth, H.C. and Kitchen, B.J., 1981. The relationship between the levels of free fatty acids, lipoprotein lipase, carboxylesterase, N-acetyl-beta D-glucosaminidase, somatic cell counts and other mastitis indices in bovine milk. J. Dairy Res. 48: 253-265.
  11. Salih, A.M. and Anderson, M., 1979. Observations on the influence of high cell count on lipolysis in bovine milk. J. Dairy Res. 46: 453-462.
  12. Shahab Lavasani, A.R. and Sherbaf, F., 2013. The Effect of Lipase Enzyme Addition on the Lipolysis of Iranian White Brine Cheese. J. School Res. Liber. 4: 105-108.
  13. ISIRI, Standard 326. 2008. Milk and its products. Sampling guide. (In Persian)
  14. ISIRI, Standard 639. 1970. Determining the amount of total nitrogen in milk. Kajdal method. (In Persian)
  15. ISIRI, Standard 2852. 2007. Milk and its products. Determination of acidity and pH test method. (In Persian)
  16. ISIRI, Standard 637. 2007. Determining milk solids. (In Persian)
  17. ISIRI, Standard 384. 2010. Milk fat measurement. (In Persian)
  18.  AOAC. 2002. Official methods of analysis of the AOAC, 15th ed. (Ed. S. Williams). Arlington, USA: Association of Official Analytical Chemists.
  19. De Jong, C. and Badings. H.T., 1990. Determination of free fatty acids in milk and cheese. Procedures for extraction, clean up and capillary gas chromatographic analysis. J. High-Resol. Chr. 13: 94-98.
  20. Shakeel, U.R., Banks, J.M., Brechany, E.Y., Muir, D.D., McSweeney, P.L.H. and Fox, P.F., 2000. Influence of ripening temperature on the volatiles profile and flavour of Cheddar cheese made from raw or pasteurized milk. Dairy J. 10: 55-65.
  21. Saeedi, , Shahab Lavasani, A. and Movahed, S., 2019. Effect of different levels of somatic cell count of cow ,s milk and lipase enzyme addition on free fatty acid composition and sensory properties of White brined cheese. Journal of Animal Environment. 11(1): 331-342. (In Persian)
  22. Molimard, P. and Spinnler, H.E., 1996. Review: Compounds Involved in the Flavor of Surface Mold Ripened Cheeses: Origins and Properties. Dairy Sci. 2: 169-184.
  23. Chen,X., Wang, J.Z., Van Kessel, J.S., Ren, F.Z. and Zeng, S.S., 2010. Effect of somatic cell count in goat milk on yield, sensory quality, and fatty acid profile of semisoft cheese. J. Dairy Sci. 93: 1345-1354.
  24. Mitchell, G.E., Fedrick, J.A. and Rogers, S.A., 1986. The relationship between somatic cell counts composition and manufacturing properties of bulk milk. 2. Cheddar cheese from farm bulk milk. J. Dairy Technol. 41: 12-14.
  25. Beresford, T.P., Fitzsimons, N.A., Brennan, N.L. and Cogan, T.M., 2001. Recent advances in cheese microbiology. Int. Dairy J. 11: 259-274.
  26. Coppola, R., Nanni, M., Iorizzo, M., Sorrentino, A., Sorrentino, E. and Chiavari, C., 2000. Microbiological characteristics of Parmigiano-Reggiano cheese during the cheesemaking and the first months of the ripening. Lait. 80: 479-490.
  27. Alizadeh,, Hamedi, M. and Khosroshahi, A., 2006. Modeling of proteolysis and lipolysis in Iranian white brine cheese. J.  Food Chem. 97: 294-301.
  28. Chávarri, F., Bustamante, M., Santisteban, ,Virto, M., Barrón, L.J.R. and  de Renobales, M., 1999. Changes in Free Fatty Acids During Ripening of Idiazabal Cheese Manufactured at Different Times of the Year. J. Dairy Sci. 82: 885-890.
  29. Virto,, Chávarri, F., Bustamante,M.A., Barron, L.J.R., Aramburu, M., Vicente, M.S., Pérez Elortondo, F.J., Albisu, M. and de Renobales, M., 2003. Lamb rennet paste in ovine cheese manufacture. Lipolysis and flavor. Int. Dairy J. 13: 391-399.
  30. Bauman, D.E. and Lock, A.L., 2006. Conjugated Linoleic Acid: Biosynthesis and Nutritional Significance. In: Fox, P.F. and McSweeney, P.L.H., Advanced Dairy Chemistry. Lipids, 3rd edition. New York. Springer. 2: 93-124.
  31. Jaeggi, J.J., Govindasamy-lucey, S., Berger, Y.M. and Johnson, M.E., 2003. Hard ewes milk manufactured from milk of three different groups of somatic cell counts. Journal of Dairy Science. 86: 3082-3089.