تأثیر دفعات غذادهی و شوری بر فاکتورهای خونی، ایمنی و شاخص های استرس بچه ماهی ازون برون (Acipenser stellatus)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران

2 موسسه تحقیقات بین المللی تاسماهیان دریای خزر، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران

3 پژوهشکده آبزی پروری آب های داخلی، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندر انزلی، ایران

چکیده

این مطالعه با هدف ارزیابی تأثیر دفعات غذادهی و شوری بر بر فاکتورهای خونی، ایمنی و شاخص ­های استرس بچه ماهی ازون برون پرورشی (Acipenser stellatus) در سال 1399 انجام شد. به همین منظور 810 بچه ماهی اوزون برون (میانگین وزن 12/24 گرم) در 9 تیمار و هریک با سه تکرار با سه سطح شوری و سه سطح دفعات غذادهی درنظر گرفته ­شدند. تیمارها شامل این موارد بودند: تیمار1: دو بار غذادهی در روز و شوری صفر، تیمار2: چهار بار غذادهی در روز و شوری صفر، تیمار3: شش بار غذادهی در روز و شوری صفر، تیمار4: دو بار غذادهی در روز و شوری 6 گرم در هزار، تیمار5: چهار بار غذادهی در روز و شوری 6 گرم در هزار، تیمار6: شش بار غذادهی در روز و شوری 6 گرم در هزار، تیمار7: دو بار غذادهی در روز و شوری 12 گرم در هزار، تیمار8: چهار بار غذادهی در روز و شوری 12 گرم در هزار و تیمار9:  شش بار غذادهی در روز و شوری 12 گرم در هزار. ماهیان به مدت هشت هفته پرورش داده شدند. سپس از ماهیان خونگیری به عمل آمد. نتایج نشان داد که در اکثر موارد تعداد گلبول­ های سفید، قرمز، اندیس های خونی در تیمارهای 4 و 6 بیش تر از سایر تیمار ها بودند (0/05>P). هم چنین میزان IgM، لیزوزیم و توتال ایمنوگلوبولین در تیمارهای 3 و 4 بیش تر از سایر تیمارها بودند (0/05>P). نتایج بررسی شاخص ­های استرس بیان کننده آن بود که میزان گلوکز و کورتیزول سرم خون ماهیان در تیمارها اختلاف معنی  دار آماری داشتند (0/05>P). با توجه به نتایج حاصله می توان گفت امکان پرورش ماهی ازون برون در شوری­ های مختلف را داشته و افزایش دفعات غذایی به این ماهی کمک می ­کند شرایط محیطی متفاوت را تحمل نماید. البته با توجه به مسایل اقتصادی و هزینه کارگر توصیه می ­شود ماهیان روزانه 4 بار در روز غذادهی شوند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of feeding frequency and salinity on blood factors, immunity and stress indicators of stellate sturgeon (Acipenser stellatus) juveniles

نویسندگان [English]

  • Seyedeh Maryam Ahamadi 1
  • Habib Vahabzadeh 1
  • Hossein Khara 1
  • Zabih Ollah Pajand 2
  • Mohammad Sayad Borani 3
1 Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran
2 International Sturgeon Research Institute of the Caspian Sea, Iranian Fisheries Sciences research, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
3 Inland Waters Aquaculture Research Center, Iranian Fisheries Sciences research, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran
چکیده [English]

The aim of this study was to evaluate the effect of feeding frequency and salinity on blood factors, immunity and stress indices of farmed stellate sturgeon (Acipenser stellatus) in 1399. For this purpose, 810 juvenile stellate sturgeon (average weight 12.24 g) in 9 treatments and each with three replications with three salinity levels and three feeding frequency levels were considered. Treatments included: Treatment 1: Twice daily feeding and zero salinity, Treatment 2: Four times daily feeding and zero salinity, Treatment 3: 6 times daily feeding and zero salinity, Treatment 4: Twice daily feeding and 6 g salinity Per thousand, treatment 5: four times a day feeding and salinity 6 grams per thousand, treatment 6: six times a day feeding and salinity 6 grams per thousand, treatment 7: twice daily feeding and salinity 12 grams per thousand, treatment 8: Four times feeding load 12 grams per day and salinity per thousand and treatment 9: 6 times per day and salinity 12 grams per thousand. The fish were reared for eight weeks. Blood samples were then taken from the fish. The results showed that in most cases, the number of white blood cells, red blood cells, blood indices in treatments 4 and 6 were higher than other treatments (P<0.05). Also, the amount of IgM, lysozyme and total immunoglobulin in treatments 3 and 4 were higher than other treatments (P<0.05). The results of stress indicators showed that the serum glucose and cortisol levels of fish had a statistically significant difference (P<0.05). According to the results, it can be said that it is possible to breed stellate sturgeon in different salinities and increasing the number of feedings helps this fish to withstand different environmental conditions. However, due to economic issues and labor costs, it is recommended to feed the fish 4 times a day.

کلیدواژه‌ها [English]

  • Stellate
  • Feeding frequency
  • Salinity
  • Blood
  • Immunity
  • Stress parameters

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فصلنامه محیط زیست جانوری
دوره 14، شماره 1
شماره بهار
اردیبهشت 1401
صفحه 179-190

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