بررسی سرواپیدمیولوژیک لپتوسپیروز در شترهای کشتار شده استان گلستان

نوع مقاله : بیماری ها

نویسنده

موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

لپتوسپیروز یک بیماری عفونی مشترک در دام  و انسان است که به وسیله ارگانیسم مارپیچی شکل (اسپروکت) از جنس لپتوسپیرا ایجاد می شود. سرووار های مهم شامل لپتوسپیرا  پومونا، کانیکولا، ایکتروهموراژیه، اتومالیس، گریپوتیفوزا و هاردجو هستند. این ارگانیسم ها میزبان های متعددی، از جمله انسان دارند. هدف از این مطالعه بررسی شیوع سرووارهای لپتوسپیروز در بین شتر های کشتار شده  استان گلستان بود. در این بررسی 235 نمونه سرم خون از ورید وداج شترها از کشتارگاه استان گلستان جمع آوری شد و با روش میکروآگلوتیناسیون مورد سنجش قرار گرفت: میزان آلودگی شترها به باکتری لپتوسپیرا در سنین، جنس و فصول مختلف با روش مربع  کای و با استفاده از نرم ­افزار SPSS بررسی شد. کل نمونه ­ها، 44 نمونه (18/72درصد) دارای پاسخ سرولوژی مثبت بود که به ترتیب  (81/82 درصد)، (11/37 درصد) و (6/81 درصد) با یک، دو و سه سرووار واکنش مثبت نشان دادند. در نمونه­ های مثبت، 42/6 درصد سرووار اتومنالیس، 31/49 در صد ایکتروهموراژِیه، 11/11 درصد سرووار کانیکولا، 3/7 درصد سرووار پومونا ، 5/55 درصد سرووار گریپوتیفوزا و سرجروهاردجو واکنش مثبت نشان دادند. بیش ترین میزان عیار آنتی بادی (38/88 درصد) درتیتراسیون 1:800 مشاهده گردید. بیش ترین فراوانی در سن 2-0 سالگی (18/8 درصد)، جنس نر (19/46 درصد) که از نظر آماری اختلاف معنی داری مشاهده نشد (0/05<P) و بیش ترین فراوانی در فصل تابستان (21/42 درصد) مشاهده گردید که از نظر آماری اختلاف معنی­ داری مشاهده شد (0/05<P). عفونت لپتوسپیرایی در سطح استان به طور فعال وجود دارد و می تواند موجب انتقال بیماری به سایر دام های سالم و انسان شود. لذا با توجه به عدم واکسیناسیون دام ها در منطقه باید واکسیناسیون و توصیه های بهداشتی و ضروری، غربالگری سالانه در دام ها و تعیین سرووار غالب در جهت پیشگیری از رخداد بیماری در این منطقه به طور کامل مورد توجه قرار گیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Seroepidemiological survey of leptospirosis in slaughtered camels in Golestan province

نویسنده [English]

  • Parastoo Poorghafoor Langroodi
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Leptospirosis is one of the most common diseases between animal and human around the world. Regarding to the death report of a farmer in Gorgan because of leptospirosis and abnormal mortalities in forest mice in Aliabad-e-Katul,it had seemed to be necessary to investigate Leptospirosis Golestan province. Therefore, this study was considered the prevalence of Leptospira interrogans in serum samples of Golestan province camels. Blood samples were collected from 235 camels of different ages in Golestan slaughterhouse and were examined by microagglutination method. The incidence of leptospirosis was investigated at different ages, sex, and seasons using chi-square method and SPSS. The results showed that from the total samples, 44 (% 18.72) samples had positive serologic response, of which 36 (% 81.82), 5 (% 11.37), and 3 (% 6.81) of samples had one, two and three positive reaction serotype, respectively. Among samples with positive serologic response, % 42.60, % 31.49, % 11.11, % 5.55 and % 3.70 showed a positive reaction on automnalis, icterohaemorrajiae, canicola serjerohardjo and gripotyphosa, and finally Pomona, respectively. The highest frequency (% 38.88) was observed in the 1: 800 Titration. The highest frequency was observed at age of 0 to 2 years old (%18.80), male (%19.46), which was not statistically significant (P>0.05). The highest frequency was observed in autumn (26.82%). Leptospirosis infection is actively present at the province. And it is widely circulated between humans and livestock. This issue is very important in the prevention of disease in livestock and ultimately the health of human society.

کلیدواژه‌ها [English]

  • Leptospirosis
  • MAT
  • Camel
  • Golestan
  1. Agésilas, F., Gey, F., Monbrunt, A., Combes, J.C., Llanas, B. and Schlossmacher, P., 2005. Acute leptospirosis in children in Reunion Island: a retrospective review of 16 cases. Arch Pediatr. 12(9): 1344-1348.
  2. Brenner, J., Kaufmann, A.F., Sulzerk, R., Steigerwalt, A.G., Rogers, F.C. and Weyant, R.S., 1999. Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi nov. and four new Leptospira genomospecies. Int. J. Syst. Bacteriol. 49: 839-858.
  3. Tabatabai, M. and Firozi, R., 2001. Bacterial diseases of livestock. Tehran University Publications. 431-435. (In Persian)
  4. Jori, F., Galvez, H., Mendoza, P., Cespedes, M. and Mayor, P., 2009. Monitoring of leptospirosis seroprevalence in a colony of captive collared peccaries (Tayassu tajacu) from the Peruvian Amazon. Research in Veterinary Science. 86: 383-387.
  5. Herrmann-Stock, C. and Brioudes, A., 2005. Retrospective review of leptospirosis in Goadeloupe, French West Indies 1994-2001.West Indian Med J. 54(1): 42-46.
  6. Tangkanakul, W. and Smits, H.L., 2005. Leptospirosis an emerging health problem in Thailand. J Trop Med Public Health. Mar. 36(2): 281-288.
  7. Tangkanakul, W., Tharmaphornpil, P., Plikaytis, BD., Bragg, S., Poonsuksombat, D. and Choomkasien, P., 2000. Risk factors associated with leptospirosis in northeastern Thailand, 1998. Am J Trop Med Hyg. 63(3-4): 204-208.
  8. Abdolahpour, G. and Shfighi, T., 2009. Serodiagnosis of leptospirosis in cattle in north of Iran, Gilan J Vet Res. 3(1): 7-10.
  9. Lau, C.L., Smythe, L.D., Craig, S.B. and Weinstein, P., 2010. Climate change, flooding, urbanisation and leptospirosis: fuelling the fire? Royal Society of Tropical Medicine and Hygiene. 104: 631-638.
  10. Radostits, O.M., Gay, C.C., HinchcliffK, W. and Constable, P.D., 2007. Veterinary Medicine. 10th Elsevier. 1094-1110.
  11. Barkin, R.M.; Gackian, I.C. and Glosser, I.W., 1974. Infection by Leptospira ballum: a laboratory associated case. South. Med. J. 67: 155-156.
  12. Vandyousefi, J., Moradibidhendi, S. and Ahoorai, P., 1995. New findings about leptospirosis in the Razi Institute. Pajouhesh & Sazandegi. 25: 72-75. (In Persian)
  13. Maghamgi, Gh., 1981. Investigation of leptospirosis in calving female cows around Tehran. Publications of the Veterinary Organization. 20: 50-60. (In Persian)
  14. Alanso-Andicoberry, C., Garcia-Pena, F., Pereira-Bueno, J., Costas, E. and Ortego-Mora, L.M., 2001. Herd-level risk factors associated with leptospira spp. Seroprevalence in dairy and beef catle in Spain. Preventive veterinary medicine. 52: 109-117.
  15. Haji Hajikolaei, M., Ghorbanpour, M. and Abdollahpour, G., 2005. Serological study of Leptospirosis in cattle in Ahvaz. J Faculty of Vet Med, Univ Tehran. 60: 7-15.
  16. Adler, B. and Pena Mocrezuma, A., Leptospira and leptospirosis. Vet microbial. 140: 287-296.
  17. Momtaz, H. and Moshkelani, S., Detection and characterization of Leptospira spp. Isolated from aborted bovine clinical samples. Acta Vet. Brno J. 81: 21-25.
  18. Hassanpour, A., Fartashvand, M., Abdollahpour, Gh., Mogaddam, Gh.A., Nadalian, M.G. and Sattari, S., 2007. Seroprevalence of leptospiral infection in dairy herds in Tabriz- Iran. Pajouhesh & Sazandegi. 74: 67-77. (In Persian)
  19. Lilenbaum, W., Morais, Z.M., Gonçales, A.P., Souza, G.O., Richtzenhain, L. and Vasconcellos, S.A., 2007. First isolation of leptospires from dairy goats in Brazil. Brazilian Journal of Microbiology. 38: 507-510.
  20. Zahtab, H., 2004. Report of the Golestan General Veterinary Department. (In Persian)
  21. Golsha, R., Khodabakhshi, B. and Rahnama, A., 2007. Leptospirosis in Golestan province in Iran (Reports of twelve cases). J Gorgan Univ Med Sci. 9(2): 76-80. (In Persian)
  22. Poorghafoor langroodi, P., 2018. Leptospirosis epidemic in cows of Golestan province during 2006-2007.Journal of Animal Environmental. 10(4): 113-118. (In Persian)
  23. Poorghafoor langroodi, P., 2020. Sero-epidemiological survey of leptospirosis in cattle Golestan Province.Journal of Animal Environmental. 11(4): 47-52. (In Persian)
  24. Benkirane, A., Noury, S., Hartskeerl, R.A., Goris, M.G., Ahmed, A. and Nally, J.E., 2016. Preliminary Investigations on the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco. Transbound Emerg Dis. 63(2):e178-84. doi: 10.1111/tbed.12252. Epub 2014 Jul 26. PMID: 25065690.
  25. Amjad Islam, A. and Ijaz, M., Leptospirosis: Rising Nuisance for cattle and threat to public health. Bacterial Cattle Disease.
  26.  OIE. 2008. 2nd Global Conference on Animal Welfare Putting the OIE Standards to Work Cairo (Egypt), 20-22 October.
  27. Bahari, A. and Abdollahpour, G., 2011. A serological survey on leptospirosis in aborted dairy cattle in industrial farms of Hamedan suburb, Iran. Iranian Journal of Veterinary Research, Shiraz university. 12(4): 37.
  28. Pinto Pda, S. and Lidonati, H., 2016. A systemic review on the microscopic agglutination test seroepidemiology of bovine leptospirosis in Latin America. Top Animal Health Pod. 48(2): 239-248.
  29. Honarmand, H.R. and Khayat, I., 2010. Isolation of phatogenic leptospires from blood samples of patients by PCR-RFLP method. J Gorgan Uni Med Sci. 12(1): 70-79.
  30. Khaki, P., Roohi, Z. and Moradi Bidhendi, S., 2014. Application of micro agglutination test in detecting serovars of Leptospira. Journal of Gorgan University of Medical Sciences. 16(3): 99-105. (In Persian)
  31. Vahedi Nouri, N., 2000. Identification of the dominant strains of Leptospira in suspected cows in Mazandaran province. Final report of the research project of Razi Institute. (In Persian)
  32. Asadpour, Y., Moradibidhendi, S., Rahimabadi, E. and Pourseyyed, S.M., 2008. A survey on prevalence of Leptospirosis and it´s serotype in livestock in Guilan province. Journal of comprative pathobiology.4(4): 299-304. (In Persian)
  33. Bueno, J., Costas, E. and Ortego-Mora, L.M., 2001. Herd level risk factors associated with leptospira spp. Seroprevalence in dairy and beef cattle in Spain. Preventive veterinary medicine. Vol. 52, pp: 109-117.
  34. Khalili, and Sakhaee, E., 2020. Serological evidence of leptospirosis in Iran; A systematic review and meta-analysis Microbial Pathogenesis. 138: 103833.