تعیین حداکثر و حداقل غلظت مجاز سولفات روی در ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss) با تاکید بر آسیب شناسی بافتی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه دانشگاه گنبدکاووس، گنبدکاووس، ایران

چکیده

 هدف از مطالعه حاضر تعیین حداکثر و حداقل غلظت مجاز سولفات­ روی در ماهی قزل ­آلای رنگین ­کمان با تاکید بر آسیب ­شناسی بافتی در مدت 96 ساعت (زمستان 1399) در آزمایشگاه آبزی­ پروری دانشگاه گنبدکاووس اجرا شد. از نمک­ سولفات ­روی جهت سمیت روی در آزمایشات استفاده شد. ماهی قزل­ آلای رنگین­ کمان با سایز 1/31±6/18 گرم انتخاب شدند. غلظت­ کشنده سولفات ­روی برای ماهی قزل­ آلای رنگین­ کمان در مدت 96 ساعت برابر 21 میلی­ گرم در لیتر به دست آمد. بر اساس شیب خط رگرسیون و منحنی درصد تلفات ماهی در غلظت­ های مختلف مورد استفاده برای ماهی قزل­ آلای رنگین­ کمان غلظت نیمه کشنده برابر 11/54 میلی­ گرم در لیتر به دست آمد. به هرحال نتایج حاصل از سمیت حاد سولفات­ روی نشان داد، نرخ مرگ و میر به­ طور مستقیم وابسته به غلظت­ های استفاده شده از سولفات روی، در حالی که در گروه شاهد مرگ و میری مشاهده نشد. در گروه شاهد تغییرات رفتاری خاصی در طول آزمایشات دیده نشد. نتایج حاضر نشان داد، عمومی ­ترین ضایعات رخ داده شده در آبشش ماهیانی که در معرض غلظت کشنده روی قرار داشتند شامل هایپرپلازی، خون ریزی، پرخونی، ادم و اتساع لاملاهای ثانویه بود. به طورکلی نتایج چنین آزمایشاتی جهت ارزیابی ریسک اکولوژیک بسیار مفید است.

کلیدواژه‌ها

عنوان مقاله [English]

Determination of MATC and LOEC of ZnSO4 in rainbow trout (Oncorhynchus mykiss) Emphasis by tissue lesion

نویسندگان [English]

  • Mohamad Farhangi
  • Hojatillah Jafaryan
  • Ziya Kordjazi
Department of Fisheries, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran
چکیده [English]

The aim of  this study conducted in Gonbad Kavous aquaculture laboratory was to  determine MATC and LOEC of ZnSO4 in fish using tissue lesion examinations. To do this, rainbow trout (Oncorhynchus mykiss) juveniles (6.18±1.33 g) were exposed zinc sulphate over 96 hours (in December 2021). Zinc sulphate salt was used to examine toxicity of zinc. The lethal dose of zinc for Oncorhynchus mykiss was 21 mgL-1 over 96 hours. According to the linear regression and relationship between the percentage of mortality and different concentrations of zinc, the LC50 (median lethal dose) was obtained 11.54 mgL-1 for Oncorhynchus mykiss. However, acute toxicity of zinc sulphate showed that mortality was  directly proportional to the concentration of the zinc sulphate, whilst there was no mortality in the control treatment. No behavioral changes were observed in control treatment over the experiment. Microscopic studies showed that the common lesions of gill in fish exposed to zinc lethal concentration were hyperplasia, edema, hyperemia, hemorrhage and expansion of secondary lamellae. Therefore, the results and information obtained in this study can be applicable in ecological risk assessment studies.

کلیدواژه‌ها [English]

  • Zinc sulphate
  • Acute toxicity
  • Mortality percentage
  • Oncorhynchus mykiss

مراجع

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فصلنامه محیط زیست جانوری
دوره 14، شماره 2
تیر 1401
صفحه 177-184

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