تأثیر سطوح مختلف آرد کرم خاکی (Eisenia fetida) بر برخی از پارامترهای رشد و رنگ‌پذیری بچه ماهی اسکار (Astronotus ocellatus)

نوع مقاله : مقاله پژوهشی


1 موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

2 گروه شیلات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران


هدف از این مطالعه ارزیابی تأثیر جایگزینی جیره تجاری با سطوح مختلف آرد کرم خاکی (0(شاهد)، 5، 10 و 15 درصد) در بچه ­ماهیان اسکار بر پارامترهای رشد و رنگ‌پذیری ماهی مذکور بود. برای این منظور آزمایشی در قالب یک طرح کاملاً تصادفی با 4 تیمار آزمایشی هر کدام با 3 تکرار طراحی شد. برای این منظور تعداد 120 قطعه بچه­ ماهی اسکار با میانگین وزنی 0/35±4/32 گرم در 12 آکواریوم ذخیره‌سازی شدند. تراکم بچه­ ماهیان در هر آکواریوم 10 عدد بود. بعد از 8 هفته از هر تیمار سه عدد بچه­ ماهی برای اندازه ­گیری خصوصیات مورد مطالعه به‌طور تصادفی انتخاب شد. نتایج نشان داد که افزودن 5 درصد آرد کرم خاکی باعث افزایش معنی‌دار وزن نهایی (0/49±18/31 گرم)، نرخ رشد ویژه (0/11±2/51 درصد در روز)، درصد بازماندگی (100/00±0 درصد) و بهترین ضریب تبدیل غذا (0/14±1/74) شد (0/05>p). میزان کاروتنوئید در تمامی تیمارهای آزمایشی نسبت به تیمار شاهد به شکل معنی‌داری بالاتر بود (0/05>p). مؤلفه‌های a* و b* در تیمارهای جایگزین شده با 5 و 10 درصد پودر کرم خاکی به شکل معنی‌داری بالاتر از گروه شاهد (0/05>p) و مؤلفه L* در سطوح مذکور (5 و 10 درصد) به شکل معنی‌داری پایین‌تر از گروه شاهد اندازه‌گیری شدند (0/05>p). مجموع نتایج این تحقیق نشان داد جایگزینی آرد کرم خاکی در سطح 5 درصد در جیره تجاری تأثیرات مثبتی بر پارامترهای رشد و فاکتورهای تعیین‌کننده کیفیت رنگ پوست بچه­ ماهیان اسکار داشت.



عنوان مقاله [English]

Effect of different levels of earthworm (Eisenia fetida) meal on some of growth parameters and skin pigmentation in Oscar (Astronotus ocellatus) fry

نویسندگان [English]

  • Kavos Nazari 1
  • Seyed Pezhman Hosseini-Shekarabi 2
  • Mehdi Khodme Sedigh Kazerani 2
1 Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
2 Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

This study aimed to investigate the effect of replacing a commercial feed with different ratios of earthworm meal (0 (Control), 5, 10, and 15%) on growth parameters and skin pigmentation in Oscar fry. For this purpose, the experiment was performed in a completely randomized design with four treatments and three replicates. One hundred and twenty of Oscar fry with an initial average weight of 4.32±0.35g were dispersed into 12 aquaria. The stocking density was 10 fingerlings per aquarium. After 8 weeks, three fish from each treatment were randomly selected to measure the studied characteristics. The highest final weight (18.31±0.49 g), specific growth rate (2.51±0.11 %/day), survival rate (100 %), and the best food conversion ratio FCR (1.74±0.14) were observed in the fish fed with 5% earthworm meal compared to others (p<0.05). Total carotenoid at all treatments was significantly higher than the control group (p<0.05). The mean level of a* and b* factors in treatments replacing with 5 and 10% of earthworm meal was significantly higher than the control group (p<0.05); While, L* factors in mentioned levels (5 and 10%) was significantly lower than the control group (p<0.05). In conclusion, the results of this study showed that replacing the commercial feed with earthworm meal at 5% had positive effects on growth parameters and skin pigmentation factors in Oscar fry.

کلیدواژه‌ها [English]

  • Ornamental fish
  • Earthworm
  • Carotenoid
  • Colorimetry
  • Growth
  • Feed efficiency
  1. Hosseini, S.P. and Shamsaei, M., 2018. Scientific and practical principles in freshwater aquariums. Islamic Azad University Publications. 214 p. (In Persian)
  2. Faria, P.M.C., Ribeiro, K., Almeida, C.F., Santos, F.W.M. and Santos, R.F.B., 2016. Aquicultura ornamental: um mercado promissor. Panorama aquiculture. 26: 24-37.
  3. Broad, S., Mulliken, T. and Roe, D., 2003. The nature and extent of legal and illegal trade in wildlife. In: The trade in wildlife, regulation for conservation. Oldfield, S., (ed), Earthscan, London, UK. 210 p.
  4. Yýlmaz, S. and Ergun, S., 2011. Effect of red pepper (Capsicum annuum) on pigmentation of blue streak hap. Aquaculture. Bamidgeh. 63: 633-639.
  5. Nelson J.S., 2006. Fishes of the World. Wiley.com
  6. Sugie, A., Terai, Y., Ota, R. and Okada, N., 2004. The evolution of genes for pigmentation in African cichlid fishes. Gene. 343: 337-346.
  7. Tavares-Dias, M. and Neves, l.R., 2017. Diversity of parasites in wild Astronotus ocellatus (Perciformes, Cichlidae), an ornamental and food fish in Brazil. Anais da Academia Brasileira de Ciências. Annals of the Brazilian Academy of Sciences. Online version ISSN 1678-2690.
  8. Froese, R. and Pauly, D., 2017. World Wide Web electronic publication. www.fishbase.org, version (02/2017).
  9. Chatzifotis, S., Pavlidis, M., Jimeno, C., Vardanis, P. and Divanach, P., 2004. The effect of carotenoid sources on skin coloration of red porgy Aquaculture Europe conference, Biotechnology for quality, Barcelona, Spain. 6: 32.
  10. Ghiasvand, Z. and Shapouri, M., 2006. The effect of synthetic and natural pigments on the colour of the Albino Oscar (Astronotus ocellatus, Agassiz, 1831). Journal of Marine Biology1(1): 78-85. (In Persian)
  11. Diler, I. and Dilek, K., 2002. Significance of pigmentation and use in aquaculture, Journal of Fisheries and Aquatic Science. 2: 97-99.
  12. Kop, A. and Durmaz, Y., 2008. The effect of synthetic and natural pigments on the colour of the cichlids (Cichlasoma severum). Aquaculture international. 16: 117-122.
  13. Sales, J. and Janssens, P.X., 2003. Nutrient requirements of ornamental fish. Aquatic Living Resources. 16(6): 533-540.
  14. Sogbesan, A.O., Ugwumba, A.A.A. and Madu, C.T., 2007. Productivity potentials and nutritional values of semi arid zone earthworm (Hyperiodrilus euryaulos; Clausen, 1967 cultured in organic wastes as fish meal supplement. Pakistan Journal of biological sciences. 10(17): 2992-2997.
  15. Barker, D., Fitzpatrick, M.P. and Dierenfeld, E.S., 1998. Nutrient composition of selected whole invertebrates. Zoo Biology. 17: 123-134.
  16. Yahyaabadi, M., 2018. Comparing the ability of two earthworms species Dendrobaena veneta and Eisenia fetida in the production of vermicompost. Journal of Animal Environmental. 9(4): 339-346. (In Persian)
  17. Velasquez, L., Ibañez, I., Herrera, C. and Oyarzun, M., 1991. A note on the nutritional evaluation of worm meal (Eisenia fetida) in diets for rainbow trout. Animal Science. 53(1): 119-122.
  18. Mahmoud Aghdam, A. and Yousefi Siahkalroodi, S., 2009. Study of consuming earthworm (Eisenia foetida) in rainbow trout (Oncorhynchus mykiss) nutrition (as base of protein source). Journal of Animal Environmental. 1(1):
    51-56. (In Persian)
  19. Mohammad Pour, Y., Shapoori, M., Sadeghi, M.S. and Gholami, Sh., 2019. A Comparison of nutritional value of Biomar diet and earthworm as feed on growth performance and survival of ornamental Oscar fish (Asteronotus ocellatus). Iranian Scientific Fisheries Journal. 28(2): 39-48. (In Persian)
  20. Razaghi, , Yeganeh, S.,  Keramat Amirkolaie, A. and Janikhalili, Kh., 2018. A Replacement of Fishmeal by Earthworm (Eisenia fetida) Meal Together with Garlic Powder on Growth Parameters, Carcass Composition and Digestibility of Rainbow Trout (Oncorhynchus mykiss). Journal of Fisheries. 70(4): 337-349. (In Persian)
  21. Seidgar, , mohebbi, F., Nekuiefard, A., Hafezieh, M., Dadgar, S., Abbaspour Anbi, A. and Shiri, S., 2019. The effect of Artemia urmiana, Earthworm, Cow heart and concentrate as supplementary diets on skin color and pigmentation of Oscar fish. International Journal of Aquatic Science. 10(2): 88-93.
  22. Kostecka, J. and Pączka, G., 2006. Possible use of earthworm Eisenia fetida (Sav.) biomass for breeding aquarium fish. European Journal of Soil 42: 231-233. https://doi.org/10.1016/j.ejsobi.2006.07.029
  23. Popek, W., Łuszczek, E. and Rościszewska, M., 1996. Influence of supplemental feeding of earthworm (Eisenia fetida) on growth, gonad maturation and spawning in gold fish. Agriculture Academy. 310: 45-53.
  24. Ahmadifard, N., Sotudeh, M. and Imani, A., 2016. Effect of commercial food replacement with earthworm (Eisenia foetida) on growth, survival, the number of larvae and their resistance to salinity stress in swordtail fish (Xiphophorus helleri). Iranian Scientific Fisheries Journal. 25(3): 231-241. (In Persian)
  25. Shahgholian, T., 2014. Color changes of bream (Paraneetroplus melanurus × Amphilophus citrinellus) fed with a diet containing earthworms raised on foods containing natural pigments. Master's thesis. Shahrekord University. 60 p.
  26. Stafford, E.A. and Tacon. A.G.J., 1988. The use of earthworm as a food for rainbow trout Salmo gairdneri. In: Earthworms in waste and environmental Edwarrds, C.A. and Neuhauser, E.F., (eds). Academic Publishing, The Hague, the Netherlands, 181-192.
  27. Leatherland, J.F. and Hilton, J.W., 1988. Thyroidal compensation in Salmo gairdneri fed canola meal. Fish physiology and biochemistry. 5(4): 199-207.
  28.  AOAC. 1999. Official methods of analysis of AOAC international (16th Ed.). Washington, D.C: AOAC, USA.
  29. Soltani, M., Omidbeigi, R., Rezvani, S., Mehrabi, M. and Chitsaz, H., 2001. Studies on the effect of the sensitizing effect of clove essential oil and extract on rainbow trout under some water quality conditions. Journal of Veterinary Research. 56(4): 85-89. (In Persian)
  30. Torrissen, O.J. and Naevdal, G., 1984. Pigmentation of salmonids genetical variation in carotenoid deposition in rainbow trout. Aquaculture. 38: 59-66.
  31. Wang, Y.J., Chien, Y.H. and Pan, C.H., 2006. Effects of dietary supplementation of carotenoids on survival, growth, pigmentation, & antioxidant capacity of characins, Hyphessobrycon callistus. Aquaculture. 260: 640-648.
  32. Afshari, H. and Farahnaki, A., 2009. The possibility of using Photoshop software to measure the color of food: color change Mazafati date in getting the synthetic pitch. Journal of Food Research. 5(1): 37-46.
  33. Xiao, H., Cui, Y., Hung, S.S.O., Zhu, X., Zou, Z. and Xie, S., 1999. Growth of juvenile Chinese sturgeon Acipenser sinensis fed live and formulated diets. North American Journal of Aquaculture. 61: 184-188.
  34. Mohsen, S.H., El-Hammady, A.K.I. and Taha, M.K.S., 2017. Evaluation of alternative animal protein sources to replace fishmeal in practical diets for African catfish (Clarias gariepinus). Egyptian Journal of Nutrition and Feeds. 20(1): 149-162.
  35. Wang, K., Qi, S., Mu, X., Chai, T., Yang, Y., Wang, D. and Wang, C., 2015. Evaluation of the toxicity, AChE activity and DNA damage caused by imidacloprid on earthworms, Eisenia fetida. Bulletin of Environmental Contamination and Toxicology. 95(4): 475-480.
  36. Kauschke, E., Mohrig, W. and Cooper, E.L., 2007. Coelomic fluid proteins as basic components of innate immunityin earthworms. European Journal of Soil Biology. 43: 110-115. DOI: 10.1016/j.ejsobi.2007.08.043.
  37. Kobayashi, H., Ohtomi, M., Sekizawa, Y. and Ohta, N., 2001. Toxicity of coelomic fluid of the earthworm Eisenia fetida to vertebrates but not invertebrates: probable role of sphingomyelin. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology. 128(3): 401-411. DOI: 10.1016/ S1532- 0456(00)00213-1.
  38. Khademi hamidi, M., Harsij, Jafaryan, H. and Farhangi, M., 2019. The effect of Earth worm (Eisenia fetida) difference levels on growth performance and body composition and Nutrition and Digestive Enzymes of Rainbow trout (Oncorhynchus mykiss). Journal of Applied Ichthyological Research. 7 (1) :117-130. (In Persian)
  39. Monebi, C.O. and Ugwumba, A.A.A., 2013. Utilization of the earthworm, Eudrilus eugeniae in the diet of Heteroclarias fingerlings. International Journal of Fisheries and Aquaculture. 5(2): 19-25.
  40. Seidgar, M., Hafezieh, M. and Nekuie fard, A., 2015. The variation in the effect of fairy shrimp, artemia and concentrate diet on pigmentation and skin color quality of goldfish. Iranian Journal of fisheries Sciences. 24: 13-25.
  41. Sogbesan, A.O. and Madu, C.T., 2008. Evaluation of earthworm (Hyperiodrilus euryaulos, Clausen, 1914; Oligocheata: eudrilidae) meal as protein feedstuff in diets for Heterobranchus longifilis fingerlings under laboratory conditions. Research J of Environmental Sciences. 2: 23-31.
  42. Dedeke, G.A., 2013. Partial replacement of fish meal by earthworm meal (Libyodrilus violaceus) in diets for African catfish, (Clarias gariepinus) International J of Research in Fisheries and Aquaculture. 5(9): 229-233.
  43. Edwards, C.A. and Lofty, J.R., 1977. Biology of Earth worms. 3rd Edition, Chapman and Hall, London.
  44. Hansen, R.P. and Czochanska, Z., 1975. The fatty acid composition of the lipids of earthworms. Journal of the Science of Food and Agriculture. 26: 961-971.
  45. Óscar Pereira, J. and Gomes, E.F., 1995. Growth of rainbow trout fed a diet supplemented with earthworms, after chemical treatment. Aquaculture International. 3: 36-42.
  46. Tacon, A.G.J., Stafford, E.A. and Edwards, C.A., 1983. A preliminary investigation of the nutritive value of three terrestrial lumbricid worms for rainbow trout. Aquaculture. 35: 187-199.
  47. Omoyinmi, A., 2012. Growth Performance of Nile Tilapia-Oreochromis niloticus Fed Diets Containing Different Sources of Animal Protein. Libyan Agriculture Research Center Journal International. 3(1): 18-23.
  48. Farahi, M., Kasiri, A., Talebi, M. and Sudagar, M., 2010. Effect of different feed types on growth, spawning, hatching and larval survival in angelfish (Pterophyllum scalare). AACL Bioflux. 3(4): 299-303.
  49. Ho, A.L.F.C., Zong, S. and Lin J., 2014. Skin color retention after dietary carotenoid deprivation and dominance mediated skin coloration in clown anemonefish, Amphiprion ocellaris. AACL Bioflux. 7(2): 103-115.
  50. Chatzifotis, S., Pavlidis, M., Jimeno, C.D., Vardanis, G., Sterioti, A. and Divanach, P., 2005. The effect of different carotenoid sources on skin coloration of cultured red porgy. Aquaculture Research. 36: 1517-1525.
  51. Wathne, E., Bjerkeng, B., Storebakken, T., Vassvik, V. and Odland, A.B., 1998. Pigmentation of Atlantic salmon (salmon salar) fed astaxanthin in all meals or in alternating meals. Aquaculture. 159: 217-231.
  52. Bjerkeng, B., 2000. Carotenoid pigmentation of salmonid fishes-recent progress. Avances en nutricion acuicola V. Memorias del V simposium internacional de nutricion acuicola. 19: 22.
  53. La Mora, G.I., Arredondo-Figueroa, J., Ponce-Palafox, J., Barriga-Soca, I.A. and Vernon-Carter, J., 2006. Comparison of red chilli (Capsicum annuum) oleoresin and astaxanthin on Oncorhynchus mykiss fillet pigmentation. Aquaculture (Amsterdam, Netherlands). 258: 487-495.
  54. Mandal, B., Mukherjee, A. and Banerjee, S., 2010. Growth and pigmentation development efficiencies in fantail guppy, Poecilla reticulate fed with commercially available feeds. Agriculture and Biology Journal of North America. 1: 1264-1267.
  55. Vahdat, S., Fereidouni, A.E. and Khalesi, M.K., 2018. Long-term effects of vermicompost manure leachate (powder) inclusions on growth and survival, biochemical composition, total carotenoids, and broodstock reproductive performance of Artemia franciscana (Kellogg, 1906). Aquaculture International. 26(2): 569-588.
  56. Shapooeri, M., Ghiasvand, Z. and Jamii, Sh., 2012. The study of synthetic and natural pigments on the color of the Albino Oscar. International Journal of Marine Science and Engineering. 2: 203-206.