تأثیر سطوح مختلف افزودنی گیاهی XTRACT جیره بر شاخص های هماتولوژیک، بیوشیمیایی خون و ایمنی بچه ماهی طلایی (Carassius auratus)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه سرا، ایران

2 گروه علوم دریایی، پژوهشکده حوضه آبی خزر، دانشگاه گیلان، رشت، ایران

چکیده

این پژوهش به منظور بررسی تاثیر سطوح مختلف ماده تجاری XTRACT جیره بر شاخص ­های هماتولوژیک، شاخص های بیوشیمیایی خون و فاکتورهای ایمنی بچه ماهی طلایی (Carassius auratus) انجام پذیرفت. بدین منظور بچه ماهیان طلایی با میانگین وزنی 0/17±7/53 گرم در 6 تیمار و سه تکرار به صورت کاملأ تصادفی در 18 آکواریوم به حجم آبگیری 80 لیتر توزیع شدند. ماده تجاری XTRACT جیره در سطوح صفر (X0)، 100 (X100)، 200 (X200)، 300(X300)، 400 (X400) و 500 (X500) میلی گرم در هر کیلوگرم جیره غذایی اضافه شد. طول دوره پرورش 12 هفته در نظر گرفته شد. برای بررسی شاخص ­های هماتولوژیک، شاخص های بیوشیمیایی خون و فاکتورهای ایمنی و هم چنین مقایسه بین تیمارها در پایان دوره پرورش عملیات خون­گیری صورت گرفت. نتایج نشان داد در بین فاکتورهای بیوشیمیایی پلاسما از قبیل گلوکز، تری گلیسرید و کلسترول در تیمار حاوی 200 میلی گرم ماده تجاری XTRACT در هر کیلوگرم اختلاف معنی دار مشاهده شد (0/05>P). بیش ترین میزان پروتئین کل، آلبومین و گلوبولین در تیمار حاوی 200 میلی گرم ماده تجاری XTRACT در هر کیلوگرم مشاهده شد که با تیمار شاهد دارای تفاوت معنی دار بود (0/05>P). بیش ترین میزان C4 در تیمار حاوی 300 میلی گرم در هرکیلوگرم ماده تجاری XTRACT مشاهده شد (0/05>P). از لحاظ شاخص های خونی از قبیل: گلبول قرمز، هموگلوبین، هماتوکریت MCV، MCH و MCHC بین تیمارهای حاوی ماده تجاری XTRACT و تیمار شاهد اختلاف معنی داری مشاهده نشد (0/05<P). بیش ترین تعداد گلبول سفید و درصد مونوسیت، لنفوسیت و نوتروفیل در تیمار حاوی 500 میلی گرم ماده تجاری XTRACT در هر کیلوگرم مشاهده شد (0/05>P). به عنوان یک نتیجه گیری کلی می توان بیان کرد که استفاده از 200 میلی گرم در کیلوگرم ماده تجاری XTRACT در جیره غذایی بچه ماهی طلایی می تواند باعث تحریک سیستم ایمنی و از این طریق به سلامتی و بهبود وضعیت فیزیولوژیک در ماهی طلایی شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Dietary XTRACT on Hematological and Biochemical Parameters, and Safety Factors Carassius Auratus

نویسندگان [English]

  • Reza Farzi Qharabolagh 1
  • Mirmasoud Sajjadi 1 2
  • Bahram Falahatkar 1 2
1 Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran
2 Department of Marine Science, the Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran
چکیده [English]

This study was conducted to evaluate the effects of dietary XTRACT on hematological parameters, blood biochemical parameters, and safety factors of goldfish (Carassius auratus). For this purpose, goldfish children with a mean weight of 7.53 ± 0.17 grams and a length of 8.34 ± 0.26 cm in 6 treatments and three replicates were distributed randomly in 18 aquariums with 80 liters watering capacity. The commercial XTRACT diet was added to diets at levels 100 (X100), 200 (X200), 300 (X300), 400 (X400), 500 (X500) mg / kg of diet and control diet without XTRACT. The breeding period was considered to be 12 weeks. The results showed a significant difference between plasma biochemical factors such as glucose, triglyceride, and cholesterol in 200 mg of XTRACT commercial substance per kg (P<0.05). The highest amount of total protein, albumin, and globulin was observed in this treatment, which significantly differs from the control treatment (P<0.05). The highest amount of C4 was observed in the 300 mg/kg of XTRACT commercial substance (P<0.05). No significant differences were revealed in Hematological parameters such as red blood cells, hemoglobin, hematocrit MCV, MCH, and MCHC (P>0.05). The highest white blood cell count and percentage of monocytes, lymphocytes, and neutrophils were observed in the t500 mg of XTRACT commercial substance per kg (P<0.05). As a general conclusion, it can be stated that the use of 200 mg / kg of commercial XTRACT in the goldfish diet can stimulate the immune system and thus improve the health and physiological status of goldfish.

کلیدواژه‌ها [English]

  • Thyme
  • Cinnamon
  • Pepper
  • Hematology
  • Glucose
  • Biochemistry

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فصلنامه محیط زیست جانوری
دوره 14، شماره 1
شماره بهار
اردیبهشت 1401
صفحه 197-204

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