تاثیر باکتریوفاژهای ضداشریشیا کلی بیماری زای پرندگان بر عملکرد تولیدی، خصوصیات لاشه و برخی فراسنجه های خونی در جوجه های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

10.22034/AEJ.2021.300228.2614

چکیده

این آزمایش به منظور بررسی تاثیر باکتریوفاژهای ضد اشریشیا کلی بر عملکرد تولیدی، خصوصیات لاشه و برخی فراسنجه های خونی در جوجه های گوشتی اجرا شد. این مطالعه با استفاده از 168 قطعه جوجه یک روزه سویه راس برای بررسی تاثیر هفت تیمار در شش تکرار و هر تکرار شامل چهار قطعه جوجه اجرا گردید. تیمارهای آزمایشی شامل تلقیح دهانی یک میلی لیتر از باکتریوفاژ TMU1، TMU2، مخلوط آن ها، تزریق عضلانی یک میلی لیتر از باکتریوفاژ TMU1، تزریق عضلانی 0/5 میلی لیتر باکتریوفاژ TMU2، تزریق عضلانی 0/5 میلی لیتر مخلوط باکتریوفاژها  TMU1 و TMU2 و گروه شاهد دریافت کننده گاواژ و تزریق محیط کشت بدون باکتریوفاژ بود. غلظت کلسیم سرم خون در جوجه های تزریق شده با باکتریوفاژ TMU1 و مخلوط باکتریوفاژها بیش تر از جوجه های گاواژ شده با مخلوط باکتریوفاژ بود. میانگین غلظت اسیدهای چرب آزاد در خون جوجه های گاواژ شده با باکتریوفاژ TMU1 بیش تر از جوجه های تزریق شده با باکتریوفاژ TMU2 بود. میانگین غلظت لیپوپروتئین های با دانسیته بالا، در جوجه های تزریق شده با مخلوط دو نوع باکتریوفاژ بیش تر از جوجه های تغذیه شده با گاواژ مخلوط باکتریوفاژها بود. فعالیت آنزیم کبدی آسپارتات آمینو ترانسفراز در جوجه های تزریق شده با باکتریوفاژ TMU2 23/90 درصد کم تر از پرندگان شاهد بود. نتیجه ­گیری شد که تلقیح دهانی و تزریق عضلانی باکتریوفاژهای ضد اشریشیا کلی بیماریزا به پرنده سالم تاثیری بر عملکرد رشد و وزن اجزای لاشه جوجه گوشتی نداشت ولی برخی فراسنجه های خون را احتمالاً در راستای سلامت پرنده تغییر داد.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of bacteriophages against avian pathogenic Escherichia coli on productive performance, carcass characteristics and certain blood parameters in broiler chicken

نویسندگان [English]

  • Farshid Rahmatei
  • Heshmatollah Khosravinia
  • Babak Masourei
  • Maryam Mirderikvandei
Department of Animal Sciences, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
چکیده [English]

This study aimed to investigate the effect of anti-Escherichia coli bacteriophages on production performance, carcass characteristics and some blood parameters in broiler chickens. A total number of 168 one-day-old Ross 308 chicks were used to exmaine the effects of 7 experimental treatments in 4 replicates of 10 birds each.  Experimental treatments include oral inoculation of 1 ml of bacteriophage TMU1, TMU2, and their mixtures, intramuscular injection of 1 ml of bacteriophage TMU1, intramuscular injection of 0.5 ml of bacteriophage TMU2, intramuscular injection of 0.5 ml of a mixture of bacteriophages TMU1 and TMU1. The control group received oral gavageing and injection of culture medium without bacteriophage. Serum calcium concentration were higher in the birds injected with TMU1 bacteriophage and mixed bacteriophages. Serum concentration of free fatty acids was greater in the birds recieving TMU1 bacteriophage than those injected with TMU2 bacteriophage. The mean concentration of high-density lipoproteins was elevated in in the birds injected with the mixture of bacteriophages compared with those fed with the same treatment. Hepatic aspartate aminotransferase activity was 23.90% lower in the chickens injected with TMU2 bacteriophage than control birds. It was concluded that oral inoculation and intramuscular injection of pathogenic anti-Escherichia coli bacteriophages into healthy birds exhibited no effect on growth performance and carcass components percentage. However, it may provides certain benefits towards birds health indicated by reduced liver enzymes activity.

کلیدواژه‌ها [English]

  • Broiler chicken
  • Escherichia coli
  • Bacteriophage
  • Productive performance
  • Liver
  • Carcass
  1. Huff, W.E., Huff, G.R., Rath, N.C., Balog, J.M., Xie, H. and Moore, J.P.A., 2002. Donoghue AM. Prevention of Escherichia coli respiratory infection in broiler chickens with bacteriophage (SPR02). Poult Sci. 81(4): 437-441.
  2. Tavassoli, S.P., Froudi, F. and Mousavi, S.N., 2013. Effects of garlic extract on yolk cholesterol levels, Egg microbial load and layer performance. J Anim Environ. 5(1): 55-61.
  3. Mousavi Parsa, D., Bazargani-Gilani, B. and Pajohi Alamoti, M., 2018. Effects of barberry extract singly and combined to Zein edible coating containing onion (Allium cepa) essential oil on Microbial Spoilage of chicken breast meat. J Anim Environ. 10(4): 187-194.
  4. Ackermann, H.W., 2003. Bacteriophage observations and evolution. Res Microbiol. 154(4): 245-251.
  5. El-Shibiny, A., Connerton, P.L. and Connerton, I.F., 2005. Enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free range and organic chickens. Appl Environ Microbiol. 71(3): 1259-1266.
  6. Doffkay, Z., Dömötör, D., Kovács, T. and Rákhely, G., 2015. Bacteriophage therapy against plant, animal and human pathogens. Acta Biol Szeged. 59(2): 291-302.
  7. Kaikabo, A.A., AbdulKarim, S.M. and Abas, F., 2017. Evaluation of the efficacy of chitosan nanoparticles loaded ΦKAZ14 bacteriophage in the biological control of colibacillosis in chickens. Poult Sci. 96(2): 295-302.
  8. Golkar, Z., Bagasra, O. and Pace, D.G., 2014. Bacteriophage therapy: a potential solution for the antibiotic resistance crisis. J Infect Dev Ctries. 8(02): 129-136.
  9. Statistical analyses system (SAS). 2003. SAS Users Guide: Statistics. Ver. 6. Cary, NC.
  10. Wang, G.Y., Tu, P., Chen, X., Guo, Y.G. and Jiang, S.X., 2013. Effect of three polyether ionophores on pharmacokinetics of florfenicol in male broilers. J Vet Pharmacol Ther. 36(5): 494-501.
  11. Huff, W.E., Huff, G.R., Rath, N.C., Balog, J.M. and Donoghue, A.M., 2004. Therapeutic efficacy of bacteriophage and Baytril (enrofloxacin) individually and in combination to treat colibacillosis in broilers. Poult Sci. 83(12): 1944-1947.
  12. Oliveira, A., Sereno, R., Nicolau, A. and Azeredo, J., 2009. In vivo toxicity study of phage lysate in chickens. Br Poult Sci. 50(5): 558-563.
  13. Lau, G.L., Sieo, C.C., Tan, W.S., Hair-Bejo, M., Jalila, A. and Ho, Y.W., 2010. Efficacy of a bacteriophage isolated from chickens as a therapeutic agent for colibacillosis in broiler chickens. Poult Sci. 89(12):
    2589-2596.
  14. Atterbury, R.J., Van Bergen, M.A., Ortiz, F., Lovell, M.A., Harris, J.A., De Boer, A., Wagenaar, J.A., Allen, V.M. and Barrow, P.A., 2007. Bacteriophage therapy to reduce Salmonella colonization of broiler chickens. Appl Environ Microbiol. 73(14): 4543-4549.
  15. Chambers, J.R. and Gong, J., 2011. The intestinal microbiota and its modulation for Salmonella control in chickens. Food Res Int. 44(10): 3149-3159.
  16. Diaz-Sanchez, S., D'Souza, D., Biswas, D. and Hanning, I., 2015. Botanical alternatives to antibiotics for use in organic poultry production. Poult Sci. 94(6):1419-1430.
  17. Noor, M., Runa, N.Y., Husna, A., Rahman, M., Rajib, D.M., Mahbub-e-Elahi, A.T. and Rahman, M.M., 2020. Evaluation of the effect of dietary supplementation of bacteriophage on production performance and excreta microflora of commercial broiler and layer chickens in Bangladesh. MOJ Proteom Bioinform. 9: 27-31.
  18. Insoft, R.M., Sanderson, I.R. and Walker, W.A., 1996. Development of immune function in the intestine and its role in neonatal diseases. Pediat Clin. 43(2): 551-571.
  19. Wernicki, A., Nowaczek, A. and Urban-Chmiel, R., 2017. Bacteriophage therapy to combat bacterial infections in poultry. Virol J. 14(1): 1-3.
  20. Clavijo, V. and Flórez, M.J., 2018. The gastrointestinal microbiome and its association with the control of pathogens in broiler chicken production: a review. Poult Sci. 97(3): 1006-10021.
  21. Zhao, J., Liu, Y., Xiao, C., He, S., Yao, H. and Bao, G., 2017. Efficacy of phage therapy in controlling rabbit colibacillosis and changes in cecal microbiota. Front Microbiol. 8: 957.
  22. Huff, W.E., Huff, G.R., Rath, N.C. and Donoghue, A.M., 2013. Method of administration affects the ability of bacteriophage to prevent colibacillosis in 1-day-old broiler chickens. Poult Sci. 92(4): 930-934.
  23. Huff, W.E., Huff, G.R., Rath, N.C., Balog, J.M. and Donoghue, A.M., 2003. Evaluation of aerosol spray and intramuscular injection of bacteriophage to treat an Escherichia coli respiratory infection. Poult Sci. 82(7):
    1108-1112.
  24. Toro, H., Price, S.B., McKee, S., Hoerr, F.J., Krehling, J., Perdue, M. and Bauermeister, L., 2005. Use of bacteriophages in combination with competitive exclusion to reduce Salmonella from infected chickens. Avian Dis. 49(1): 118-124.
  25. Whichard, J.M., Sriranganathan, N. and Pierson, F.W., 2003. Suppression of Salmonella growth by wild-type and large-plaque variants of bacteriophage Felix O1 in liquid culture and on chicken frankfurters. J Food Prot. 66(2):
    220-225.
  26. Carvalho, C.M., Gannon, B.W., Halfhide, D.E., Santos, S.B., Hayes, C.M., Roe, J.M. and Azeredo, J., 2010. The in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of Campylobacter coli and Campylobacter jejuni in chickens. BMC Microbiol. 10(1): 1-1.
  27. Barrow, P., Lovell, M. and Berchieri, J.A., 1998. Use of lytic bacteriophage for control of experimental Escherichia coli septicemia and meningitis in chickens and calves. Clin Diagn Lab Immunol. 5(3): 294-298.
  28. Lim, T.H., Kim, M.S., Lee, D.H., Lee, Y.N., Park, J.K., Youn, H.N., Lee, H.J., Yang, S.Y., Cho, Y.W., Lee, J.B. and Park, S.Y., 2012. Use of bacteriophage for biological control of Salmonella Enteritidis infection in chicken. Res Vet Sci. 93(3): 1173-1178.
  29. Wright, A., Hawkins, C.H., Änggård, E.E. and Harper, D.R., 2009. A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic‐resistant Pseudomonas aeruginosa; a preliminary report of efficacy. Clin Otolaryngol. 34(4): 349-357.
  30. Kutter, E., De Vos, D., Gvasalia, G., Alavidze, Z., Gogokhia, L., Kuhl, S. and Abedon, S.T., 2010. Phage therapy in clinical practice: treatment of human infections. Curr Pharm Biotechnol. 11(1): 69-86.
  31. Kim, J.H., Kim, J.W., Shin, H.S., Kim, M.C., Lee, J.H., Kim, G. and Kil, D.Y., 2015. Effect of dietary supplementation of bacteriophage on performance, egg quality and caecal bacterial populations in laying hens. Br Poult Sci. 56(1): 132-136.
  32. Clavijo, V., Baquero, D., Hernandez, S., Farfan, J.C., Arias, J., Arévalo, A., Donado-Godoy, P. and Vives-Flores, M., 2019. Phage cocktail SalmoFREE® reduces Salmonella on a commercial broiler farm. Poult Sci. 98(10): 5054-5063.