اثر افزودن مخلوط روغن سیر و آفتابگردان بر فراسنجه‌های تولید گاز و جمعیت پروتوزوآ در شرایط برون تنی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بخش تحقیقات علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان چهارمحال و بختیاری، سازمان تحقیقات، آموزش و ترویج کشاورزی، شهرکرد، ایران

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

10.22034/AEJ.2021.283226.2515

چکیده

این پژوهش با هدف مطالعه اثر مخلوط روغن سیر و روغن آفتابگردان در جیره‌ دارای نسبت مساوی کنسانتره به علوفه بر پویایی تخمیر در شرایط برون‌تنی انجام­ شد. مخلوط روغن سیر و روغن آفتابگردان در سطح‌ 2 درصد ماده خشک جیره استفاده شد. فراسنجه­ های تولید گاز، pH، غلظت نیتروژن آمونیاکی و جمعیت پروتوزوآ اندازه‌گیری شد. انرژی قابل متابولیسم، اسیدهای چرب کوتاه زنجیر و گوارش‌پذیری ماده آلی برآورد شد. نتایج نشان داد که مخلوط روغن سیر و روغن آفتابگردان باعث افزایش تولید گاز شد (128/64 در برابر 108/51 میلی‌لیتر، 0/05˂P)، اما اثری بر  سرعت گاز، نیمه عمر تولید گاز و فاز تأخیر نداشت. افزودن مخلوط روغن سیر و روغن آفتابگردان به جیره باعث افزایش گوارش‌پذیری ماده آلی برآورد شده (80/40 در برابر 75/05 درصد)، غلظت اسیدهای چرب فرار برآورده شده (1/60 در برابر 1/47 میلی‌مول) و انرژی قابل متابولیسم برآورد شده (12/20 در برابر 11/38 مگاژول در کیلوگرم ماده خشک) در مقایسه با جیره شاهد شد (0/05˂P) . غلظت نیتروژن آمونیاکی، pH، کل جمعیت پروتوزوآ و جنس­ های انتودینیوم، دیپلودینیوم و افریواسکولکس تحت تأثیر استفاده از مخلوط روغن سیر و روغن آفتابگردان قرار نگرفتند. براساس نتایج این آزمایش، استفاده از مخلوط روغن سیر و روغن آفتابگردان در سطح‌ 2 درصد ماده خشک جیره  نه تنها اثر منفی بر پویایی تخمیر شکمبه نداشت، بلکه باعث بهبود برخی فراسنجه‌های تخمیر شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Addition of Mixture of Garlic Oil and Sunflower Oil on In Vitro Gas Production Parameters and Protozoa Population

نویسندگان [English]

  • Golnaz Taasoli 1
  • Shahryar Kargar 2
1 Department of Animal Science, Chahatmahal Bakhtiari Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shahrekord, Iran
2 Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran
چکیده [English]

This experiment was aimed to study the effect of mixture of garlic oil and sunflower oil in diet containing equal ratio of concentrate to forage. The mixture of garlic oil and sunflower oil was added at 2% of diet DM. Gas production parameters, pH, N-ammonia and total protozoa population were measured and metabolizable energy (ME), short -chain fatty acid (SCFA) and organic matter digestibility (OMD) was estimated. The results showed that the mixture of garlic oil and sunflower oil increased gas production (128.64 vs 108.51 mL, P<0.05) but had no significant effect on gas production rate, half-life and lag phase. The addition of the mixture of garlic oil and sunflower oil to diet, increased OMD, SCFA and ME in compare to the control diet (P<0.05). The concentration of Ammonia-N, pH, total protozoa population, Entodinium, Diplodinium and Ophryoscolex population did not affected by the inclusion of the oil mixture. It is concluded that the addition of a mixture of garlic oil and sunflower oil not only had no negative effect on rumen fermentation but also, improved some of the fermentation parameters.

کلیدواژه‌ها [English]

  • Digestion parameters
  • Garlic oil
  • In vitro gas production
  • Sunflower oil
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