Document Type : Genetic
Authors
1
Department of environmental sciences and Energy, Azad university, Science and Research Branch of Tehran
2
Department of Fisheries and Environmental Science, Faculty of Environmental Science, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran
3
Faculty of Natural Resources, University of Tehran,karaj, Iran
Abstract
For over two decades molecular biology has created dramatic evolutionary in ecological science and It has provided abundant new information about ecology and evolution of plants, animals, bacteria, alga and mushrooms.
Molecular markers has contributed a lot in quantitative of genetic diversity, displacement of individual and gene exchange between populations, inbreeding, identification of individuals, recognition new species, determination historical distribution pattern of species.
A lot of molecular analysis is done on the different variety of species in the world, in this research for the first time molecular studies has been done about survey and comparision of genetic diversity intra and between populations of grey francolin in south of Iran till with study on sequence of part of the mitochondrial gene be revealed diversity intra and between populations.
This study based on survey of genetic diversity in part of mitochondrial gene named D-loop, which is in mitochondria.
Samples were collected of south of Iran from different parts of Hormozgan and Kerman province. Samples included tissues, feather and blood of this bird. Then DNA was extracted with salt method and phenol- chloroform methods, and then production was PCR and when the obvious band was seen on the agarose jel, it was sequenced.
In this research, results evaluated with help of Mega5, Network, Seqscape softwares and It shows two different populations and six haplotypes. The first population has scattered in Roodan, Minab, Kahnooj and Jiroft, the second population has scattered around the Bandar abbas.
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