Evaluation of allelic polymorphism of myostatin gene and its association with growth and carcass traits in Mazandaran native chicken

Document Type : Genetic

Authors

1 Department of Animal Sciences, International Campus, Ferdowsi University of Mashhad, PO Box: 64955

2 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, PO Box: 91775

3 Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, POBox: 15739

4 Agricultural Research, Training and Promotion Center, Golestan Agricultural and Natural Resources Research and Education Center, PO Box:

Abstract

This study aimed to study polymorphism of myostatin gene promoter and its association with growth and carcass traits in Mazandaran native chicken. For this purpose, 200 male chicks of Mazandaran native fowls reared in the same conditions and all were slaughtered at 12 weeks of age, were used. Traits before and after slaughter were measured and recorded. Before the destruction of all birds, blood sample and extraction of DNA from samples were performed by Cinnagen kit. Then, the desired locus of gene was amplified using specific primers and genotyped using PCR-RFLP method by specific enzyme. Phenotypic and genotypic data analysis conducted using statistical software of SAS9.2. The frequency of each allele (+) and (-) were estimated to be 0.77 and 0.23 in myostatin locus, respectively. Hardy-Weinberg equilibrium study using Chi-square test showed that the study population in loci is not in balance. The results showed a significant association between myostatin gene genotypes and traits of live weights at 12 weeks of age, carcass weight, heart weight and gizzard weight (P <0.05). Based on the results of this research can be concluded that myostatin gene can be used as a candidate for growth and carcass traits in Mazandaran native fowls breeding programs.

Keywords

References

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Journal of Animal Environment
Volume 9, Issue 4
January 2018
Pages 109-112

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