Comparison antibacterial activity gelatin nanoparticles and gelatin yellowfin tuna (Thunnus albacares)and determination minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC)

Document Type : Biology(Animal)

Authors

1 Department of Marine Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran, POBox: 775-14515

2 Institute of Fisheries Research, Agricultural Research and Training Organization, Tehran, POBox: 6116-14155

3 Islamic Azad University, Pharmaceutical Sciences Branch, Tehran, Iran

Abstract

With the now diseases and increasing in the antibiotics has been greatly improved to deal with the increasing drug resistance of pathogens. Nanotechnology is a useful way. The aim of this article is to evaluate the gelatin antibacterial property and gelatin nanoparticles against pathogenic bacteria such as E. coli, S. aureus, and P. aueroginosa and were evaluated by calculation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Gelatin and gelatin nanoparticles were extracted from yellowfin tuna. For antibacterial assay gelatin was disc diffusion and serial dilution methods. For gelatin nanoparticles antibacterial assay was turbidimetric method. Then evaluated antibacterial characters and MIC MBC were determinated. (Comparison gelatin with gelatin nanoparticles antibacterial characters showed that gelatin nanoparticles had antibacterial characteristics against S. aureus, and P. aueroginosa and gelatin had property against P.aueroginosa. Gelatin nanoparticles had lower than MIC and MBC gelatin (P=0/0 24).Gelatin nanoparticles had better antibacterial property than gelatin. The smaller the Gelatin nanoparticles (GNP), the more antibacterial characters (P< 05/0). GNP could be used pharmaceuticals and have antibacterial properties.

Keywords

References

  1. Alizadeh, H.; Salouti, M. and Shapouri, R.,2013. Intramacrophage antimicrobial effect of silver nanoparticles agaist Brucella melitensis16M.Scientialranica Journal. Vol. 3, N. 20, pp: 1035-1038.
  2. Cho, M.; Gub, Y. and Kima, S., 2006. Extracting optimization and physical properties of yellowfin tuna (Thunnus albacares) skin gelatin compared to mammalian gelatins. Food Hydrocolloids. Vol. 4, No. 19, pp: 221-229.
  3. Darroudi, M.; Mansor, B.; Hakimi, M.; Zamiri, R.; Khorasani zak, A. and Zargar, M., 2013. Preparation, characterization, and antibacterial activity of silver nanoparticles in aqueous gelatin. International Journal of Minerals, Metallurgy, and Materials. Vol. 4, No. 1, pp: 403-409.
  4. Guillen, R.; Espinosa, B.; Meza, R.; Espinoza, N.; Sanchcz, L. and Chacaltana, J., 2010. Antin1icrobial Susceptibility of Brucella melitensis Isolates in Peru. Antimicrobim acienrs and ciemotiieaiy. Vol. 3, No. 12,
    pp: 1279-1281.
  5. Lifeng, Qi.; Zirong, Xu.; Xia, J.; Caihong, H. and Xiangfei, Z., 2004. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate Research. Vol. 1, No. 339, pp: 2693-2700.
  6. Kim, S., 2006. Bioactive compound from marine process by product a review. Food Research International. Vol. 4, No. 39, pp: 383-393.
  7. Mei, N.; Xuguang, L.; Jinming, D.; Husheng, J.; Liqiao, W. and Bingshe X., 2009. Antibacterial activity of Chitosan coated Ag loaded nano SioR2R composites.Carbohydrate Polimers. Vol. 4, No. 78, pp: 54-59.
  8. Muhammad, A. and Hee, J., 2014. Antibacterial and Antiyeast Compounds from Marine-Derived Bacteria. Drug Journal. Vol. 5, No. 12, pp: 2913-2921.
  9. Nester, E.; Anderson, D.; Robert, E.; Pearshall, N. and Nester, M.T., 2003. Microbiology. A human prospect.
    Vol. 3, No.14, pp: 518-521.
  10. Qi, L.; Xu, Z.; Jiang, X.; Hu, C. and Zou, X., 2004. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate research. Vol. 1, No. 339, pp: 2693-2700.
  11. Raafat, D.; Bargen, V.; Hass, A. and Sahi, H., 2008, Insights into the mode of action of chitosan as an antibacterial compound. Applied Environment Microbiology. Vol. 12, No. 74, pp: 3764-3773.
  12. Salem, W.; Leitner, D.; Zingl, F.; Schratter, G.; Prassl, R.; Goessler, W.; Reidl, J. and Schild, S., 2015. Antibacterial activity of silver and zinc nanoprticles against Vibrio cholerae and enterotoxic Escherichia coli. International journal of medical microbiology. Vol. 1, No. 305, pp: 85-95.
  13. Vorgelegt, V., 2006. Gelatin nanoparticles as delivery system for nucleotide-based drugs. Hotverleg pub.
    USA. 963 p.
Journal of Animal Environment
Volume 9, Issue 4
January 2018
Pages 167-172

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