Evaluation of immunogenicity of bivalent vaccine streptococosis / Lactococcosis in rainbow trout (oncorhynchus mykiss)

Document Type : Disease

Authors

1 Department of Clinical sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz- Iran

2 Department of basic sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz- Iran

3 Department of Pathobiology, Faculty of Veterinary, Shahid Chamran University of Ahvaz, Ahvaz- Iran

4 Department of Clinical Sciences, Faculty of Veterinary, Shahid Chamran University of Ahvaz, Ahvaz- Iran

Abstract

In this study the Hemato- immunological responses of rainbow trout (Oncorhynchus mykiss) to vaccination with bivalent lactococcal/streptococcal vaccine were investigated. Two hundred and forty rainbow trout fingerlings (Weighing 30±1.7) were randomly divided into two equal groups in triplicates. Fish in vaccinated treatment intraperitonealy injected with vaccine on day zero and booster vaccine were directed by immersion route on day 30th of study. The control group was injected with sterile PBS, identical to vaccinated group. Blood and head kidney samples were collected from vaccinated and non-vaccinated groups at days 0, 14. 30, 45 and 60 of study. Hematological parameters including: hematocrit, hemoglobin levels, red blood cell count and White blood cell count as well as immunological parameters (Lysosim and complement activity, phagocytosis rate of head kidney macrophages) were investigated and compared among the groups. Results showed that no differences were observed in hematological parameters (P>0.05) except  the number of white blood cell count which significant increase in in vaccinated group  were seen compared with the control group. All evaluated immunological parameters of vaccinated group were significantly higher in vaccinated group (p < 0.05). It can be concluded that vaccination of rainbow trout by bivalent vaccine  against streptococcus/lactococcus disease can stimulate immune response of fish until 60 days post immunization without any side effect on hematological parameters. Then this procedure of vaccination seems suitable for rainbow trout.

Keywords


  1. Alishahi, M.; Esmaeili Rad, A.; Zarei, M. andGhorbanpour, M., 2014. Effect of dietary chitosan on immune response and disease resistance of Cyprinus carpio. Iranian Journal of Veterinary Medicine. Vol. 8, No. 2,
    pp: 125-133.
  2. Alishahi, M.; Ranjbar, M.M.; Ghorbanpour, M.; Peyghan, R.; Mesbah, M. and Razijalali, M., 2010. Effects of dietary Aloe vera on specific and nonspecific immunity of Common carp (Cyprinuscarpio). Journal of Veterinary Researches. Vol. 4, No. 3, pp: 85-91.
  3. Anderson, D.P. and Jeney, G., 1991. Responses to in vitroand in vivoimmunizations with Aeromonas salmonicida O antigen bacterins in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology.Vol. 1, pp: 251-260.
  4. Andrew, C.; céline, G.; bjarne, G.; Hansen, M.; Horne, A. and Ellis, E., 2002. Antibody increases phagocytosis and killing of Lactococcus garvieae by rainbow trout (Oncorhynchus mykiss, L.) macrophages Fish & Shellfish Immunology.Vol. 12, pp: 181-186.
  5. Barta, O., 1993. Veterinary clinical immunology laboratory. Bar-Lab Inc. 350 P.
  6. Bercovier, H.; Ghittino, C. and Eldar, A., 1997. Immunization with bacterial antigens: Infections with streptococci and related organisms. In, Gudding R, Lillehaugh A, Midtlyng PJ, Brown F (Eds): Fish Vaccinology. SW. 160 P.
  7. Brown, L.L.; Iwama, G.K. and Evelyn, T.P.T., 1996. The effects of early exposure of Coho salmon (Oncorhynchus kisutch) eggs to the p57 protein of Renibacterium salmoninarum on the development of immunity to the pathogen. Fish and Shellfish Immuno.  Vol. 6, pp: 149-165.
  8. Chen, X.; Wu, Z. And Yin, J., 2003. Effects of four species of herbs on immune function of Carassius auratus gibelio. J Fish Sci China. Vol.10, pp: 36-40.
  9. Choi, S.H.; Park, K.H.; Yoon, T.J.; Kim, J.B.; Jang, Y.S. and Choe, C.H., 2008. Dietary Koren mistletoe enhances cellular non- specific immune responses and survival of Japanese eel (Anguilla japonica). Fish Shellfish Immunol. Vol. 24, pp: 67-73.
  10. Ellis, A.E., 1990. Lysozyme assays. In: Stolen JS, Fletcher TC, Anderson DP, Roberson BS, van Muiswinkel WB, editors. Techniques in fish immunology, Fair Haven NJ: SOS Publications. pp: 101-103.
  11. Faghani, T.; Azari Takami, Gh.; Kousha, A. and Faghani, S., 2008. Survening on Alginic acid and anti-streptococcus vaccine effect on the growth performance, survival rate, hematological parameters in rainbow trout (Oncorhynchous mykiss). World Journal of Zoology. Vol. 3, No.  2, pp: 54-58.
  12. Feldman, B.F.; Zinkl, J.G. and Jain, N.C., 2000. Schalm's Veterinary Hematology. 5th ed. Lippincott Williams & Wilkins. Vol. 1124, pp: 67-73.
  13. Figueras, A.; Santarém, M.M. and Novoa,B., 1997. Phagocytic activity of turbot (Scophthalmus maximus) leucocytes: opsonic effect of antibody and complementFishandShellfishImmunology. Vol. 7, pp: 621-624.
  14. Huang, H.Y.; Chen, Y.C.; Wang, P.C.; Tsai, M.A.; Yeh, S.C.; Liang, H.J. and Chen, S.C., 2014. Efficacy of a formalin-inactivated vaccine against Streptococcus iniae infection in the farmed grouper Epinephelus coioides by intraperitoneal immunization. Vaccine. Vol. 5, No. 32, pp: 7014-7020.
  15. Li, J.; Siyuan, M.; Norman, Y. and Woo, S., 2016.Vaccination of Silver Sea Bream (Sparus sarba) against Vibrio alginolyticus: Protective Evaluation of Different Vaccinating Modalities Int. J. Mol. Sci. Vol. 17, No. 40,
    pp: 33-40.
  16. MacArthur, J.I.; Fletcher, T.C.; Pirie, B.; Davidison, R.J.L. and Thompson,A.W., 1984. Peritonea1 inflammatory cells in plaice, Pleuronectes platessa L.: effects of stress and endotoxin. J Fish Biol. Vol. 25, pp: 69-81.
  17. Mcnulty, T.S.; Klesius, P. and Shoemaker, C., 2003. Hematological Changes in Nile Tilapia (Oreochromis niloticus) Infected with Streptococcus iniae by Nare Inoculation. Journal of the World Aquaculture Society.
    Vol. 34, No.3, pp: 418-422.
  18. Pridgeon, J.W. and Klesius, P.H., 2011. Development and efficacy of a novobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus). Vaccine. Vol. 29, pp: 5986-5993.
  19. Romalde, J.L.; Luzardo-Alvarez,A.; Ravelo, C.; Toranzo, AE. and Blanco-Mendez, J., 2004. Oral immunization using alginate micro-particles as a useful strategy for booster vaccination against fish lactococcosis. Aquaculture. Vol. 236, pp: 119-129. 
  20. Sakai, D.K., 1984. Opsonization by fish antibody and complement in the immune phagocytosis by peritoneal exudate cells isolated from salmonid fishes. Journal of FishDiseases. Vol.7, pp: 29-38.
  21. Santarém, M.M. and Figueras, A., 1994. Kinetics of phagocytic activity, plaque-forming cells and specific agglutinins of turbot (Scophthalmus maximus L.) immunized with O-antigen of Vibrio damsela and Pasteurella piscicida. Fishand Shellfish Immunology.Vol. 4, pp: 527-537.
  22. Santarém, M.M. and Figueras, A., 1995. Leucocyte numbers and phagocytic activity in turbot Scophthalmus maximus following immunization with Vibrio damsela and Pasteurella piscicida 0-antigen bacterins diseases of aquatic organisms. Vol. 23, pp: 213-220.
  23. Secombes, C.J., 1990. Isolation of salmonid macrophages and analysis of their killing activity. In Techniques in Fish Immunology (J. S. Stolen, T. C. Fletcher, D. P. Anderson, B. S. Robertson & W. B. Muiswinkel, eds) U.S.A. 154 P.
  24. Soltani, M.; Pirali Kheirabadi, E.; Taheri Mirghaed, A.; Zargar, A.; Mohamadian, S.; Roohollahi, Sh. and Zakian, M., 2015.Study on Streptococcosis and Lactococcosis Outbreaks in Rainbow Trout Farms in Fars and Lorestan Provinces. JofVeterinaryMicrobiology. Vol. 30, pp: 49-58.
  25. Soltani, M.; Alishahi, M.; Mirzargar, S. and Nikbakht, G., 2007. Vaccination of rainbow trout against Streptococcus iniae infection:  comparison of different routes of administration and different vaccines.  Iranian Journal of Fisheries Sciences. Vol. 7, No. 1, pp: 129-140.
  26. Soltani, M.; Jamshidi, Sh. and Sharifpour, I., 2005. Streptococcosis caused by Streptococcus iniae in farmed rainbow trout (O. mykiss) in Iran: biophysical characteristics and pathogensis. Bulletin of European Association of Fish Pathologists. Vol. 25, pp: 95-107.
  27. Soltani, M.; Pirali Kheirabadi, E.; Taheri, M.A.; Zargar, A.; Mohamadian, S.; Roohollahi, Sh. and Zakian,
    M., 2015.
    Study on Streptococcosis and Lactococcosis Outbreaks in Rainbow Trout Farms in Fars and Lorestan Provinces.  JofVeterinaryMicrobiology. Vo. 30, pp: 49-58.
  28. Suzukl, Y. and Hibiya, T., 1986. Dynamics of leucocytic inflammatory responses in carp. Fish Path. Vol. 23,
    pp: 179-184.
  29. Thrall, M.A.; Baker, D.C. and Lassen, ED., 2004. Veterinary Hematology and Clinical Chemistry: Text and Clinical Case Presentations Set. John Wiley & Sons. UK, London. pp: 241-402.
  30. Vendrell, D.; Balcazar, J.L.; Ruiz-Zarzuela, I.; Ignacio, d.B.; Girones, O. and Muzquiz, JL., 2006. Lactococcus garvieae in fish: Review. Comparative Immunol Microbiol Infectious Dis. Vol. 29, pp:177-198
  31. Wang, E.; Wang, J.; Long, B.; Kaiyu, W.; Yang H.; Qian.; Huang, H.; Ouyang, P. and Lai, H., 2016. Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae. Sci. Rep. Vol. 6, 29310 p.