القای آسیب ژنتیکی در ماسل آب شیرین Anodonta cygnea تحت مواجهه با نانو اکسید روی

نوع مقاله : بوم شناسی

نویسندگان

گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، صندوق پستی: 487-49175

چکیده

با توجه به کاربردهای زیستی گسترده نانوذرات روی طی سال­ های اخیر، نگرانی­ های جدی در خصوص اثرات این مواد بر سلامت محیط زیست ایجاد گشته است. در پژوهش حاضر، به ارزیابی پتانسیل ژنوتوکسیک نانوذرات اکسید روی در ماسل Anodonta cygneaبه ­عنوان گونه ­ای با اهمیت اکولوژیکی بالا پرداخته شد. بدین­ منظور، ماسل ­ها به ­مدت 14 روز در معرض غلظت­ های 0 (شاهد)، 2/5، 25 و 50 میلی­ گرم بر لیتر نانو اکسید روی قرار گرفتند. جهت ارزیابی آسیب DNA از طریق الکتروفورز ژل سلول منفرد، از بافت­ های آبشش و هپاتوپانکراس طی روزهای 7 و 14 مواجهه نمونه ­برداری به ­عمل آمد. هم­ چنین آزمون ریزهسته­ ها با استفاده از بافت آبشش ماسل­ ها طی روزهای 7 و 14 انجام و فراوانی ریزهسته ­ها تعیین گردید. با توجه به نتایج، مواجهه با نانوذرات اکسید روی منجر به القای بروز ریزهسته­ ها و آسیب DNA در A. cygneaگردید به ­نحوی که فراوانی ریزهسته­ ها و مقادیر پارامترهای طول، مومنتوم و درصد DNA دنباله و هم­ چنین شاخص آسیب ژنتیکی در تمامی تیمارهای مواجهه یافته با نانوذرات اکسید روی در مقایسه با گروه شاهد افزایش یافت. هم­ چنین با افزایش غلظت و زمان مواجهه با نانوذرات اکسید روی، روند افزایشی در میزان آسیب وارد شده مشاهده شد. در مقایسه بین بافت ­های مورد بررسی نیز، بافت هپاتوپانکراس حساسیت بالاتری نسبت به سمیت ژنتیکی نانو اکسید روی نشان داد. با توجه به نتایج حاصل از پژوهش حاضر می ­توان بیان داشت که نانو اکسید روی دارای اثرات ژنوتوکسیک بالقوه بر ماسل A. cygnea می ­باشد.   

کلیدواژه‌ها

عنوان مقاله [English]

Genetic damage induction in freshwater mussel Anodonta cygnea under zinc oxide nanoparticles

نویسندگان [English]

  • Hadise Kashiri
  • Sara Jafari
  • Amir Qadermarzi
Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, PO Box: 487-49175
چکیده [English]

Considering the wide biological application of zinc nanoparticles during the recent years, serious concerns have been emerged about the effects of these materials on environmental health. In the present study, the genotoxic potential of zinc oxide nanoparticles were investigated in the mussel Anodonta cygne as an ecologically important species. For this, the mussels were exposed to 0, 2.5, 25 and 50 mg L-1 zinc oxide nanoparticles during 14 days. To assess the DNA damage through single cell gel electrophoresis, sampling was done from the gill and hepatopancreas tissues on the 7th and 14th days of exposure. Micronucleus assay was also done using the gill tissue on the 7th and 14th days and the micronuclei frequency was determined. According to the results, exposure to zinc oxide nanoparticles led to the micronuclei induction and DNA damage in A. cygnea so that the micronuclei frequency and the values of the parameters of tail length, moment and DNA percentage as well as the genetic damage index increased in all treatments exposed to zinc oxide nanoparticles as compared to the control. An increasing trend in the induced damage was also observed with the increase in exposure concentration and time. In comparing the investigated tissues, the hepatopancreas tissue exhibited more sensitivity to the genotoxicity of zinc oxide nanoparticles. According to the results from the present study, it could be stated that zinc oxide nanoparticles have potential genotoxic effects on the mussel A. cygnea.

کلیدواژه‌ها [English]

  • DNA damage
  • micronucleus
  • Mussel
  • Nano zinc oxide

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فصلنامه محیط زیست جانوری
دوره 10، شماره 1
فروردین 1397
صفحه 345-352

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