القای آسیب ژنتیکی در ماسل آب شیرین Anodonta cygnea تحت مواجهه با نانو اکسید روی

نوع مقاله: بوم شناسی

نویسندگان

گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، صندوق پستی: 487-49175

چکیده

با توجه به کاربردهای زیستی گسترده نانوذرات روی طی سال­ های اخیر، نگرانی­ های جدی در خصوص اثرات این مواد بر سلامت محیط زیست ایجاد گشته است. در پژوهش حاضر، به ارزیابی پتانسیل ژنوتوکسیک نانوذرات اکسید روی در ماسل Anodonta cygneaبه ­عنوان گونه ­ای با اهمیت اکولوژیکی بالا پرداخته شد. بدین­ منظور، ماسل ­ها به ­مدت 14 روز در معرض غلظت­ های 0 (شاهد)، 2/5، 25 و 50 میلی­ گرم بر لیتر نانو اکسید روی قرار گرفتند. جهت ارزیابی آسیب DNA از طریق الکتروفورز ژل سلول منفرد، از بافت­ های آبشش و هپاتوپانکراس طی روزهای 7 و 14 مواجهه نمونه ­برداری به ­عمل آمد. هم­ چنین آزمون ریزهسته­ ها با استفاده از بافت آبشش ماسل­ ها طی روزهای 7 و 14 انجام و فراوانی ریزهسته ­ها تعیین گردید. با توجه به نتایج، مواجهه با نانوذرات اکسید روی منجر به القای بروز ریزهسته­ ها و آسیب DNA در A. cygneaگردید به ­نحوی که فراوانی ریزهسته­ ها و مقادیر پارامترهای طول، مومنتوم و درصد DNA دنباله و هم­ چنین شاخص آسیب ژنتیکی در تمامی تیمارهای مواجهه یافته با نانوذرات اکسید روی در مقایسه با گروه شاهد افزایش یافت. هم­ چنین با افزایش غلظت و زمان مواجهه با نانوذرات اکسید روی، روند افزایشی در میزان آسیب وارد شده مشاهده شد. در مقایسه بین بافت ­های مورد بررسی نیز، بافت هپاتوپانکراس حساسیت بالاتری نسبت به سمیت ژنتیکی نانو اکسید روی نشان داد. با توجه به نتایج حاصل از پژوهش حاضر می ­توان بیان داشت که نانو اکسید روی دارای اثرات ژنوتوکسیک بالقوه بر ماسل A. cygnea می ­باشد.   

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