اثرات سمیت سلولی، همولیز و ضدانعقادی عصاره خاره چسب Siphonaria carbo

نوع مقاله: سایر

نویسندگان

1 گروه زیست فناوری دریا، دانشکده علوم و فنون دریایی، دانشگاه خلیج فارس، بوشهر، ایران

2 پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

بررسی کنونی به ­منظور تعیین ویژگی سمیت سلولی عصاره ­های استخراجی از شکم ­پای Siphonaria carbo انجام شد. میزان سمیت عصاره ­های آبی و اتانولی این گونه به­وسیله آزمون سمیت یاخته ­ای با استفاده از آرتمیا انجام شد. هم ­چنین اثرات انعقادی و لیزکنندگی روی گلبول­ های قرمز خون انسان بررسی گردید. نمونه ­ها از صخره­ های سواحل بوشهر جمع­ آوری شد. دو روش عصاره ­گیری از بافت تر و خشک با استفاده از حلال­ های آبی و اتانولی مورد استفاده قرار گرفت. نتایج آزمون سمیت یاخته­ ای نشان ­دهنده این بود که همه عصاره ­ها (به ­جز عصاره آبی خشک) اثر کشندگی داشتند و میزان سمیت با افزایش غلظت عصاره همبستگی داشت. عصاره آبی تر بیش­ ترین میزان سمیت (5/98= LC50-24hr) را در مقایسه با سایر عصاره­ ها داشت. نتایج به ­دست آمده از تست همولیز خونی نشان داد اثر همولیتیک معنی­ داری در عصاره ­های استخراجی وجود ندارد. هم­ چنین آزمون ­های انعقادی نشان دادند خاصیت ضدانعقادی در عصاره­ های استخراجی وجود ندارد. به­ طورکلی در بررسی کنونی خاصیت ضدانعقادی قوی مشاهده نشد، ولی بالا بودن اثر کشندگی سلولی عصاره­ ها، نشان ­دهنده احتمال بالای سایر اثرات مانند اثرات ضد توموری و ضد انگلی می ­باشد. روش ­های تخلیص عصاره­ ها به­ منظور دستیابی به کاربردهای دارویی عصاره پیشنهاد می­ گردد. 

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