بررسی اثر محافظت کنندگی پروتئین های نوترکیب VP28 و VP19 یک جدایه ویروس لکه سفید به روش خوراکی در برابر ویروس بیماری لکه سفید در میگوی سفید غربی

نوع مقاله: بیماری ها

نویسندگان

1 پژوهشکده آبزی پروری جنوب کشور، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران

2 مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

3 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شهید چمران، اهواز، ایران

4 گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه شهید چمران، اهواز، ایران

چکیده

   ویروس بیماری لکه سفید یکی از آسیب ­رسان­ ترین ویروس‌ها در پرورش میگوی جهان و ایران است. پروتئین­ های سطحی این ویروس در مراحل اولیه برخورد با سلول­ های میزبان نقش بسیار مهمی دارند و کاندیدی برای ساخت واکسن­ هایزیر واحد یا نوترکیب محسوب می ­شوند. این مطالعه به­ منظور دستیابی به این پروتئین ­ها جهت انجام ایمن­ سازی میگو وانامی طراحی گردید. ژنوم ویروس لکه سفید از میگوهای بیمار و دارای علائم بالینی مزارع پرورش میگوی چوئبده آبادان استخراج گردید. پس از تکثیر و خالص ­سازی، ژن پروتئین‌هایVP28 وVP19در باکتری TG1 کلون شدند. بیان پروتئین بررسی و پلیت ­های تجاری توسط باکتری حاوی پروتئین نوترکیب غیرفعالشده پوشانده شد. پست لاروهای مرحله 30 میگوی سفید غربی با پلت­ های نوترکیب تغذیه شده و در روزهای 9 و 23 پس از تغذیه، با ویروس لکه سفید چالش شدند. نتایج آزمایش مواجهه اول  نشان داد که کم­ترین درصد تلفات در گروه­ های مختلف مربوط به گروه VP28 برابر 3/84±30% و بالاترین درصد مربوط به گروه TG1 برابر 2/93±72/22% بود. در آزمایش مواجهه دوم کم ­ترین درصد تلفات مربوط به گروهVP28  5/09±%50و بالاترین درصد مربوط به گروه TG1 2/22±75/55% بود. با توجه به نتایج  تحقیق پروتئین نوترکیب VP28 قابلیت ایجاد محافظت را در برابر ویروس لکه سفید  دارد درحالی­ که پروتئین نوترکیب VP19 قابلیت محافظت را نخواهد داشت. هم ­چنین میزان بازماندگی ارتباط مستقیم با مدت زمان در اختیار داشتن پروتئین نوترکیب دارد.
 

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