ارزیابی فلوسایتومتریک اسپرم گاو بعد از انجماد- یخ گشایی در رقیق کننده منی حاوی اتانول

نوع مقاله : فیزیولوژی (جانوری)

نویسندگان

1 گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 گروه علوم طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران گروه جنین شناسی، مرکز تحقیقات زیست پزشکی تولید مثل، پژوهشگاه رویان، تهران، ایران

3 گروه علوم دامی، دانشکده کشاورزی، دانشگاه اراک، اراک، ایران

چکیده

هدف از انجام این مطالعه بررسی اثر افزودن اتانول به رقیق­ کننده بر کیفیت اسپرم گاو بعد از انجماد و یخ­ گشایی بوده است.در مجموع تعداد 24 انزال از چهار گاو نر (شش انزال از هر گاو طی سه هفته) جمع ­آوری شد. در هر تکرار، نمونه­ های منی با هم مخلوط و سپس به دو قسمت مساوی تقسیم شده و هریک با یکی از رقیق­ کننده­ های حاوی تیمارهای آزمایشی رقیق و منجمد شدند. تیمارهای مورد استفاده در این مطالعه شامل: 1) رقیق ­کننده فاقد اتانول به­ عنوان گروه شاهد (C) و 2) رقیق­ کننده­ حاوی 0/25 درصد اتانول (E) بود. پس از یخ‌ گشایی نمونه‌ها، فراسنجه ­های کیفی اسپرم شامل جنبایی، یکپارچگی و عملکرد غشای پلاسمایی، وضعیت زنده‌مانی و فعالیت میتوکندری مورد ارزیابی قرار گرفت. نتایج آزمایش حاضر نشان داد که اسپرم­ های گروه شاهد نسبت به اسپرم­ های گروه اتانول دارای جنبایی کل بالاتر (2/49±76/17 در مقابل 1/96±68/50) و میزان آپوپتوزیس کم ­تری (0/36±10/56 در مقابل 0/33±13/13) بودند (0/05>p). هم­ چنین، در سایر فراسنجه­ های کیفی اسپرم شامل یکپارچگی و عملکرد غشای پلاسمایی اسپرم و فعالیت میتوکندری، تفاوتی بین دو گروه شاهد و اتانول مشاهده نشد (0/05>p). نتایج این مطالعه نشان می ­دهد که اتانول می ­تواند اثر منفی بر فراسنجه­ های اسپرم گاو بعد از انجماد و یخ­ گشایی بگذارد.

کلیدواژه‌ها

عنوان مقاله [English]

Folw cytometric evaluation of frozen-thawed bull semen in a semen extender containing ethanol

نویسندگان [English]

  • Sayed Mohammad Hadi Hussaini 1
  • Mahdi Zhandi 1
  • Ahmad Zare-Shahneh 1
  • Mohsen Sharafi 2
  • Mjtaba Emamverdi 1
  • Mahdi Khodaeimotlagh 3
1 Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University,Tehran, Iran Department of Embryology at Reproduction Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran.
3 Department of Animal Science, Faculty of Agriculture, University of Arak, Arak, Iran
چکیده [English]

The aim of the present study was to investigate the effect of ethanol on bull sperm quality after freeze thawing process. Treatments used in this study included: 1) semen extender without ethanol as control group (C) and 2) semen extender containing 0.25% ethanol (E). A total of 24 ejaculations were collected from four healthy mature Holstein bulls in 3 consecutive weeks and twice a week. In each replicate, semen samples were mixed to eliminate individual differences and then divided into two equal parts and each of them were extended with one of the aforementioned extenders. Afterward the treatments were frozen using a standard protocol. After thawing, sperm motion characteristics, plasma membrane integrity and functionality, apoptosis status and mitochondrial activity were assessed. The result of this study was showed that spermatozoa in control group had higher total motility (68.5±1.96 vs 76.17±2.49) and lower apoptosis (10.65±0.36 vs 13.13±0.33) in comparison with group treated with ethanol (p≤0.05). Also, no differences were observed in plasma membrane integrity and functionality and mitochondrial activity between two treatments (p≥0.05). In conclusion, the results showed that ethanol could negatively affect post thaw characteristics of bull spermatozoa.

کلیدواژه‌ها [English]

  • Apoptosis
  • motility
  • Semen quality
  • mitochondria

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فصلنامه محیط زیست جانوری
دوره 10، شماره 1
فروردین 1397
صفحه 47-52

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